EFFECT OF TEMPERATURE ON TWO ENZYMES FROM A PSYCHROPHILIC CHLOROMONAS (CHLOROPHYTA) 1
ABSTRACT A psychrophilic green alga belonging to the Chloromonas genus and here named ANT1 was collected in Antarctica. The activities of two enzymes, nitrate reductase and argininosuccinate lyase, were measured at various temperatures and compared to the corresponding enzyme activities in the mesop...
Published in: | Journal of Phycology |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
1996
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1111/j.0022-3646.1996.00276.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.0022-3646.1996.00276.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.0022-3646.1996.00276.x |
id |
crwiley:10.1111/j.0022-3646.1996.00276.x |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1111/j.0022-3646.1996.00276.x 2024-06-02T07:58:20+00:00 EFFECT OF TEMPERATURE ON TWO ENZYMES FROM A PSYCHROPHILIC CHLOROMONAS (CHLOROPHYTA) 1 Loppes, Roland Devos, Nathalie Willem, Sabine Barthélemy, Pascal Matagne, René F. 1996 http://dx.doi.org/10.1111/j.0022-3646.1996.00276.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.0022-3646.1996.00276.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.0022-3646.1996.00276.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Phycology volume 32, issue 2, page 276-278 ISSN 0022-3646 1529-8817 journal-article 1996 crwiley https://doi.org/10.1111/j.0022-3646.1996.00276.x 2024-05-03T11:43:21Z ABSTRACT A psychrophilic green alga belonging to the Chloromonas genus and here named ANT1 was collected in Antarctica. The activities of two enzymes, nitrate reductase and argininosuccinate lyase, were measured at various temperatures and compared to the corresponding enzyme activities in the mesophilic species Chlamydomonas reinhardtii Dangeard. For both enzymes, the temperature for apparent optimal activity was about 20°C lower in ANT1 than in C. reinhardtii. The enzymes were also submitted to various heat treatments before measuring their activities. Both psychrophilic enzymes were more sensitive to heat than the corresponding mesophilic enzymes. It is worth stressing, however, that in both species nitrate reductase was much more sensitive to heat than argininosuccinate lyase, which probably indicates that the peculiar structure of each protein primarily determines its dependence to temperature. Secondary adaptations to low temperatures should then occur to confer the psychrophilic character. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Journal of Phycology 32 2 276 278 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
ABSTRACT A psychrophilic green alga belonging to the Chloromonas genus and here named ANT1 was collected in Antarctica. The activities of two enzymes, nitrate reductase and argininosuccinate lyase, were measured at various temperatures and compared to the corresponding enzyme activities in the mesophilic species Chlamydomonas reinhardtii Dangeard. For both enzymes, the temperature for apparent optimal activity was about 20°C lower in ANT1 than in C. reinhardtii. The enzymes were also submitted to various heat treatments before measuring their activities. Both psychrophilic enzymes were more sensitive to heat than the corresponding mesophilic enzymes. It is worth stressing, however, that in both species nitrate reductase was much more sensitive to heat than argininosuccinate lyase, which probably indicates that the peculiar structure of each protein primarily determines its dependence to temperature. Secondary adaptations to low temperatures should then occur to confer the psychrophilic character. |
format |
Article in Journal/Newspaper |
author |
Loppes, Roland Devos, Nathalie Willem, Sabine Barthélemy, Pascal Matagne, René F. |
spellingShingle |
Loppes, Roland Devos, Nathalie Willem, Sabine Barthélemy, Pascal Matagne, René F. EFFECT OF TEMPERATURE ON TWO ENZYMES FROM A PSYCHROPHILIC CHLOROMONAS (CHLOROPHYTA) 1 |
author_facet |
Loppes, Roland Devos, Nathalie Willem, Sabine Barthélemy, Pascal Matagne, René F. |
author_sort |
Loppes, Roland |
title |
EFFECT OF TEMPERATURE ON TWO ENZYMES FROM A PSYCHROPHILIC CHLOROMONAS (CHLOROPHYTA) 1 |
title_short |
EFFECT OF TEMPERATURE ON TWO ENZYMES FROM A PSYCHROPHILIC CHLOROMONAS (CHLOROPHYTA) 1 |
title_full |
EFFECT OF TEMPERATURE ON TWO ENZYMES FROM A PSYCHROPHILIC CHLOROMONAS (CHLOROPHYTA) 1 |
title_fullStr |
EFFECT OF TEMPERATURE ON TWO ENZYMES FROM A PSYCHROPHILIC CHLOROMONAS (CHLOROPHYTA) 1 |
title_full_unstemmed |
EFFECT OF TEMPERATURE ON TWO ENZYMES FROM A PSYCHROPHILIC CHLOROMONAS (CHLOROPHYTA) 1 |
title_sort |
effect of temperature on two enzymes from a psychrophilic chloromonas (chlorophyta) 1 |
publisher |
Wiley |
publishDate |
1996 |
url |
http://dx.doi.org/10.1111/j.0022-3646.1996.00276.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.0022-3646.1996.00276.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.0022-3646.1996.00276.x |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Journal of Phycology volume 32, issue 2, page 276-278 ISSN 0022-3646 1529-8817 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.0022-3646.1996.00276.x |
container_title |
Journal of Phycology |
container_volume |
32 |
container_issue |
2 |
container_start_page |
276 |
op_container_end_page |
278 |
_version_ |
1800741648049635328 |