18S RIBOSOMAL DNA SEQUENCES INDICATE A MONOPHYLETIC ORIGIN OF CHAROPHYCEAE 1
ABSTRACT Nuclear‐encoded small‐subunit (18S) ribosomal RNA genes of Chara australis R. Brown (C. corallina var. nobilis f. nobilis R.D.W.) and Nitella flexilis (L.) Ag. were amplified by polymerase chain reaction, cloned, and completely sequenced. Using structural criteria, the sequences were aligne...
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Online Access: | http://dx.doi.org/10.1111/j.0022-3646.1994.00490.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.0022-3646.1994.00490.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.0022-3646.1994.00490.x |
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crwiley:10.1111/j.0022-3646.1994.00490.x 2024-06-02T08:10:48+00:00 18S RIBOSOMAL DNA SEQUENCES INDICATE A MONOPHYLETIC ORIGIN OF CHAROPHYCEAE 1 Ragan, Mark A. Parsons, Thomas J. Sawa, Takashi Straus, Neil A. 1994 http://dx.doi.org/10.1111/j.0022-3646.1994.00490.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.0022-3646.1994.00490.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.0022-3646.1994.00490.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Phycology volume 30, issue 3, page 490-500 ISSN 0022-3646 1529-8817 journal-article 1994 crwiley https://doi.org/10.1111/j.0022-3646.1994.00490.x 2024-05-03T10:55:26Z ABSTRACT Nuclear‐encoded small‐subunit (18S) ribosomal RNA genes of Chara australis R. Brown (C. corallina var. nobilis f. nobilis R.D.W.) and Nitella flexilis (L.) Ag. were amplified by polymerase chain reaction, cloned, and completely sequenced. Using structural criteria, the sequences were aligned with 18S ribosomal DNAs (rDNAs) from 11 other chlorophyll b‐ containing algae and six higher plants (embryophytes). Phylogenetic trees were inferred by distance, neighbor‐joining, parsimony, and maximum‐likelihood approaches; confidence intervals were estimated by bootstrapping, and nonrandomness of tree structure was confirmed by permutation tests. 18S rDNAs of C. australis and two Nitella species were found to be specifically related and, together with 18S rDNAs of Chlorokybus atmophyticus Geitler, Klebsormidium flaccidum (A. Br.) Silva, Mattox, et Blackwell, and two Coleochaete species, support a robust monophyletic group (Charophyceae). Although most trees favored a specific sister‐group relationship between Charophyceae and embryophytes, statistical tests revealed that a sister‐group relationship between Charophyceae and Chlorophyceae could not be ruled out. Additional complete sequences from 18S rDNAs of lower land plants may be required to resolve phylogenetic relationships among these organisms. Article in Journal/Newspaper Nitella flexilis Wiley Online Library Journal of Phycology 30 3 490 500 |
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Wiley Online Library |
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English |
description |
ABSTRACT Nuclear‐encoded small‐subunit (18S) ribosomal RNA genes of Chara australis R. Brown (C. corallina var. nobilis f. nobilis R.D.W.) and Nitella flexilis (L.) Ag. were amplified by polymerase chain reaction, cloned, and completely sequenced. Using structural criteria, the sequences were aligned with 18S ribosomal DNAs (rDNAs) from 11 other chlorophyll b‐ containing algae and six higher plants (embryophytes). Phylogenetic trees were inferred by distance, neighbor‐joining, parsimony, and maximum‐likelihood approaches; confidence intervals were estimated by bootstrapping, and nonrandomness of tree structure was confirmed by permutation tests. 18S rDNAs of C. australis and two Nitella species were found to be specifically related and, together with 18S rDNAs of Chlorokybus atmophyticus Geitler, Klebsormidium flaccidum (A. Br.) Silva, Mattox, et Blackwell, and two Coleochaete species, support a robust monophyletic group (Charophyceae). Although most trees favored a specific sister‐group relationship between Charophyceae and embryophytes, statistical tests revealed that a sister‐group relationship between Charophyceae and Chlorophyceae could not be ruled out. Additional complete sequences from 18S rDNAs of lower land plants may be required to resolve phylogenetic relationships among these organisms. |
format |
Article in Journal/Newspaper |
author |
Ragan, Mark A. Parsons, Thomas J. Sawa, Takashi Straus, Neil A. |
spellingShingle |
Ragan, Mark A. Parsons, Thomas J. Sawa, Takashi Straus, Neil A. 18S RIBOSOMAL DNA SEQUENCES INDICATE A MONOPHYLETIC ORIGIN OF CHAROPHYCEAE 1 |
author_facet |
Ragan, Mark A. Parsons, Thomas J. Sawa, Takashi Straus, Neil A. |
author_sort |
Ragan, Mark A. |
title |
18S RIBOSOMAL DNA SEQUENCES INDICATE A MONOPHYLETIC ORIGIN OF CHAROPHYCEAE 1 |
title_short |
18S RIBOSOMAL DNA SEQUENCES INDICATE A MONOPHYLETIC ORIGIN OF CHAROPHYCEAE 1 |
title_full |
18S RIBOSOMAL DNA SEQUENCES INDICATE A MONOPHYLETIC ORIGIN OF CHAROPHYCEAE 1 |
title_fullStr |
18S RIBOSOMAL DNA SEQUENCES INDICATE A MONOPHYLETIC ORIGIN OF CHAROPHYCEAE 1 |
title_full_unstemmed |
18S RIBOSOMAL DNA SEQUENCES INDICATE A MONOPHYLETIC ORIGIN OF CHAROPHYCEAE 1 |
title_sort |
18s ribosomal dna sequences indicate a monophyletic origin of charophyceae 1 |
publisher |
Wiley |
publishDate |
1994 |
url |
http://dx.doi.org/10.1111/j.0022-3646.1994.00490.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.0022-3646.1994.00490.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.0022-3646.1994.00490.x |
genre |
Nitella flexilis |
genre_facet |
Nitella flexilis |
op_source |
Journal of Phycology volume 30, issue 3, page 490-500 ISSN 0022-3646 1529-8817 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.0022-3646.1994.00490.x |
container_title |
Journal of Phycology |
container_volume |
30 |
container_issue |
3 |
container_start_page |
490 |
op_container_end_page |
500 |
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1800756719350972416 |