Biotic and abiotic factors shape the microbiota of wild‐caught populations of the arbovirus vector Culicoides imicola
Abstract Biting midges of the genus Culicoides are known vectors of arboviruses affecting human and animal health. However, little is known about Culicoides imicola microbiota and its influence on this insect’s biology. In this study, the impact of biotic and abiotic factors on C. imicola microbiota...
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Online Access: | http://dx.doi.org/10.1111/imb.12526 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fimb.12526 http://onlinelibrary.wiley.com/wol1/doi/10.1111/imb.12526/fullpdf |
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crwiley:10.1111/imb.12526 2024-09-15T18:01:20+00:00 Biotic and abiotic factors shape the microbiota of wild‐caught populations of the arbovirus vector Culicoides imicola Díaz‐Sánchez, S. Hernández‐Jarguín, A. Torina, A. Fernández de Mera, I. G. Estrada‐Peña, A. Villar, M. La Russa, F. Blanda, V. Vicente, J. Caracappa, S. Gortazar, C. de la Fuente, J. H2020 COllaborative Management Platform for detection and Analyses of (Re-) emerging and foodborne outbreaks in Europe (COMPARE) the Italian Ministry of Health 2018 http://dx.doi.org/10.1111/imb.12526 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fimb.12526 http://onlinelibrary.wiley.com/wol1/doi/10.1111/imb.12526/fullpdf en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Insect Molecular Biology volume 27, issue 6, page 847-861 ISSN 0962-1075 1365-2583 journal-article 2018 crwiley https://doi.org/10.1111/imb.12526 2024-08-27T04:26:31Z Abstract Biting midges of the genus Culicoides are known vectors of arboviruses affecting human and animal health. However, little is known about Culicoides imicola microbiota and its influence on this insect’s biology. In this study, the impact of biotic and abiotic factors on C. imicola microbiota was characterized using shotgun‐metagenomic sequencing of whole‐body DNA samples. Wild‐caught C. imicola adult nulliparous females were sampled in two locations from Sicily, Italy. The climatic variables of temperature and soil moisture from both localities were recorded together with potential host bloodmeal sources. Shared core microbiome among C. imicola populations included Pseudomonas , Escherichia , Halomonas , Candidatus Zinderia, Propionibacterium , and Schizosaccharomyces . Specific and unique taxa were also found in C. imicola from each location, highlighting similarities and differences in microbiome composition between the two populations. DNA and protein identification showed differences in host preferences between the two populations, with Homo sapiens and Canis lupus familiaris L. being the preferred bloodmeal source in both locations. A principal component analysis showed that the combined effect of host preferences ( H. sapiens ) and local soil moisture factors shape the microbiome composition of wild‐caught populations of C. imicola . These results contribute to characterizing the role of the microbiome in insect adaptation and its utility in predicting geographic expansion of Culicoides species with potential implications for the control of vector‐borne diseases. Article in Journal/Newspaper Canis lupus Wiley Online Library Insect Molecular Biology 27 6 847 861 |
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Wiley Online Library |
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English |
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Abstract Biting midges of the genus Culicoides are known vectors of arboviruses affecting human and animal health. However, little is known about Culicoides imicola microbiota and its influence on this insect’s biology. In this study, the impact of biotic and abiotic factors on C. imicola microbiota was characterized using shotgun‐metagenomic sequencing of whole‐body DNA samples. Wild‐caught C. imicola adult nulliparous females were sampled in two locations from Sicily, Italy. The climatic variables of temperature and soil moisture from both localities were recorded together with potential host bloodmeal sources. Shared core microbiome among C. imicola populations included Pseudomonas , Escherichia , Halomonas , Candidatus Zinderia, Propionibacterium , and Schizosaccharomyces . Specific and unique taxa were also found in C. imicola from each location, highlighting similarities and differences in microbiome composition between the two populations. DNA and protein identification showed differences in host preferences between the two populations, with Homo sapiens and Canis lupus familiaris L. being the preferred bloodmeal source in both locations. A principal component analysis showed that the combined effect of host preferences ( H. sapiens ) and local soil moisture factors shape the microbiome composition of wild‐caught populations of C. imicola . These results contribute to characterizing the role of the microbiome in insect adaptation and its utility in predicting geographic expansion of Culicoides species with potential implications for the control of vector‐borne diseases. |
author2 |
H2020 COllaborative Management Platform for detection and Analyses of (Re-) emerging and foodborne outbreaks in Europe (COMPARE) the Italian Ministry of Health |
format |
Article in Journal/Newspaper |
author |
Díaz‐Sánchez, S. Hernández‐Jarguín, A. Torina, A. Fernández de Mera, I. G. Estrada‐Peña, A. Villar, M. La Russa, F. Blanda, V. Vicente, J. Caracappa, S. Gortazar, C. de la Fuente, J. |
spellingShingle |
Díaz‐Sánchez, S. Hernández‐Jarguín, A. Torina, A. Fernández de Mera, I. G. Estrada‐Peña, A. Villar, M. La Russa, F. Blanda, V. Vicente, J. Caracappa, S. Gortazar, C. de la Fuente, J. Biotic and abiotic factors shape the microbiota of wild‐caught populations of the arbovirus vector Culicoides imicola |
author_facet |
Díaz‐Sánchez, S. Hernández‐Jarguín, A. Torina, A. Fernández de Mera, I. G. Estrada‐Peña, A. Villar, M. La Russa, F. Blanda, V. Vicente, J. Caracappa, S. Gortazar, C. de la Fuente, J. |
author_sort |
Díaz‐Sánchez, S. |
title |
Biotic and abiotic factors shape the microbiota of wild‐caught populations of the arbovirus vector Culicoides imicola |
title_short |
Biotic and abiotic factors shape the microbiota of wild‐caught populations of the arbovirus vector Culicoides imicola |
title_full |
Biotic and abiotic factors shape the microbiota of wild‐caught populations of the arbovirus vector Culicoides imicola |
title_fullStr |
Biotic and abiotic factors shape the microbiota of wild‐caught populations of the arbovirus vector Culicoides imicola |
title_full_unstemmed |
Biotic and abiotic factors shape the microbiota of wild‐caught populations of the arbovirus vector Culicoides imicola |
title_sort |
biotic and abiotic factors shape the microbiota of wild‐caught populations of the arbovirus vector culicoides imicola |
publisher |
Wiley |
publishDate |
2018 |
url |
http://dx.doi.org/10.1111/imb.12526 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fimb.12526 http://onlinelibrary.wiley.com/wol1/doi/10.1111/imb.12526/fullpdf |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Insect Molecular Biology volume 27, issue 6, page 847-861 ISSN 0962-1075 1365-2583 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/imb.12526 |
container_title |
Insect Molecular Biology |
container_volume |
27 |
container_issue |
6 |
container_start_page |
847 |
op_container_end_page |
861 |
_version_ |
1810438486369501184 |