Development of a rapid method for codfish identification in processed fish products based on SYBR Green real‐time PCR
Summary Using species outside the order Gadiformes to prepare codfish products has been widely revealed as a new type of fraud in China, highlighting the necessity to establish proper methods for codfish species identification. This work aimed to develop a new SYBR Green real‐time PCR assay for the...
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crwiley:10.1111/ijfs.14446 2024-09-15T18:07:18+00:00 Development of a rapid method for codfish identification in processed fish products based on SYBR Green real‐time PCR Xiong, Xiong Yuan, Fangying Huang, Manhong Cao, Min Xiong, Xiaohui National Natural Science Foundation of China 2019 http://dx.doi.org/10.1111/ijfs.14446 https://onlinelibrary.wiley.com/doi/pdf/10.1111/ijfs.14446 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/ijfs.14446 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor International Journal of Food Science & Technology volume 55, issue 4, page 1843-1850 ISSN 0950-5423 1365-2621 journal-article 2019 crwiley https://doi.org/10.1111/ijfs.14446 2024-08-30T04:11:17Z Summary Using species outside the order Gadiformes to prepare codfish products has been widely revealed as a new type of fraud in China, highlighting the necessity to establish proper methods for codfish species identification. This work aimed to develop a new SYBR Green real‐time PCR assay for the detection of codfish ingredient (mainly the eight Gadiformes species, Gadus morhua , Gadus macrocephalus , Theragra chalcogramma , Pollachius virens , Melanogrammus aeglefinus , Merluccius merluccius , Merluccius australis and Albatrossia pectoralis ) in processed fish products based on mitochondrial 12S rRNA gene. Results highlighted the positive Cq (cycle quantification value, average value 19.18 ± 1.49) only from the eight Gadiformes species, with no fluorescent signal (Cq > 32) for the other sixty‐seven non‐Gadiformes species. The absolute limit of detection was 0.048 ng genomic DNA. Methodology validation succeeded in twenty‐six commercial products. These results demonstrated that the newly developed method is suitable for the identification of codfish ingredient in processed fish products. Article in Journal/Newspaper Gadus morhua Theragra chalcogramma Wiley Online Library International Journal of Food Science & Technology 55 4 1843 1850 |
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Wiley Online Library |
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English |
description |
Summary Using species outside the order Gadiformes to prepare codfish products has been widely revealed as a new type of fraud in China, highlighting the necessity to establish proper methods for codfish species identification. This work aimed to develop a new SYBR Green real‐time PCR assay for the detection of codfish ingredient (mainly the eight Gadiformes species, Gadus morhua , Gadus macrocephalus , Theragra chalcogramma , Pollachius virens , Melanogrammus aeglefinus , Merluccius merluccius , Merluccius australis and Albatrossia pectoralis ) in processed fish products based on mitochondrial 12S rRNA gene. Results highlighted the positive Cq (cycle quantification value, average value 19.18 ± 1.49) only from the eight Gadiformes species, with no fluorescent signal (Cq > 32) for the other sixty‐seven non‐Gadiformes species. The absolute limit of detection was 0.048 ng genomic DNA. Methodology validation succeeded in twenty‐six commercial products. These results demonstrated that the newly developed method is suitable for the identification of codfish ingredient in processed fish products. |
author2 |
National Natural Science Foundation of China |
format |
Article in Journal/Newspaper |
author |
Xiong, Xiong Yuan, Fangying Huang, Manhong Cao, Min Xiong, Xiaohui |
spellingShingle |
Xiong, Xiong Yuan, Fangying Huang, Manhong Cao, Min Xiong, Xiaohui Development of a rapid method for codfish identification in processed fish products based on SYBR Green real‐time PCR |
author_facet |
Xiong, Xiong Yuan, Fangying Huang, Manhong Cao, Min Xiong, Xiaohui |
author_sort |
Xiong, Xiong |
title |
Development of a rapid method for codfish identification in processed fish products based on SYBR Green real‐time PCR |
title_short |
Development of a rapid method for codfish identification in processed fish products based on SYBR Green real‐time PCR |
title_full |
Development of a rapid method for codfish identification in processed fish products based on SYBR Green real‐time PCR |
title_fullStr |
Development of a rapid method for codfish identification in processed fish products based on SYBR Green real‐time PCR |
title_full_unstemmed |
Development of a rapid method for codfish identification in processed fish products based on SYBR Green real‐time PCR |
title_sort |
development of a rapid method for codfish identification in processed fish products based on sybr green real‐time pcr |
publisher |
Wiley |
publishDate |
2019 |
url |
http://dx.doi.org/10.1111/ijfs.14446 https://onlinelibrary.wiley.com/doi/pdf/10.1111/ijfs.14446 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/ijfs.14446 |
genre |
Gadus morhua Theragra chalcogramma |
genre_facet |
Gadus morhua Theragra chalcogramma |
op_source |
International Journal of Food Science & Technology volume 55, issue 4, page 1843-1850 ISSN 0950-5423 1365-2621 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/ijfs.14446 |
container_title |
International Journal of Food Science & Technology |
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55 |
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4 |
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1843 |
op_container_end_page |
1850 |
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1810444684030377984 |