Triploid Pacific oysters exhibit stress response dysregulation and elevated mortality following heatwaves
Abstract Polyploidy has been suggested to negatively impact environmental stress tolerance, resulting in increased susceptibility to extreme climate events. In this study, we compared the genomic and physiological response of diploid (2 n ) and triploid (3 n ) Pacific oysters ( Crassostrea gigas ) t...
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Online Access: | http://dx.doi.org/10.1111/gcb.16880 https://onlinelibrary.wiley.com/doi/pdf/10.1111/gcb.16880 |
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crwiley:10.1111/gcb.16880 2024-10-20T14:08:16+00:00 Triploid Pacific oysters exhibit stress response dysregulation and elevated mortality following heatwaves George, Matthew N. Cattau, Olivia Middleton, Mollie A. Lawson, Delaney Vadopalas, Brent Gavery, Mackenzie Roberts, Steven B. 2023 http://dx.doi.org/10.1111/gcb.16880 https://onlinelibrary.wiley.com/doi/pdf/10.1111/gcb.16880 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Global Change Biology volume 29, issue 24, page 6969-6987 ISSN 1354-1013 1365-2486 journal-article 2023 crwiley https://doi.org/10.1111/gcb.16880 2024-10-07T04:30:56Z Abstract Polyploidy has been suggested to negatively impact environmental stress tolerance, resulting in increased susceptibility to extreme climate events. In this study, we compared the genomic and physiological response of diploid (2 n ) and triploid (3 n ) Pacific oysters ( Crassostrea gigas ) to conditions present during an atmospheric heatwave that impacted the Pacific Northwestern region of the United States in the summer of 2021. Climate stressors were applied either singly (single stressor; elevated seawater temperature, 30°C) or in succession (multiple stressor; elevated seawater temperature followed by aerial emersion at 44°C), replicating conditions present within the intertidal over a tidal cycle during the event. Oyster mortality rate was elevated within stress treatments with respect to the control and was significantly higher in triploids than diploids following multiple stress exposure (36.4% vs. 14.8%). Triploids within the multiple stressor treatment exhibited signs of energetic limitation, including metabolic depression, a significant reduction in ctenidium Na + /K + ATPase activity, and the dysregulated expression of genes associated with stress response, innate immunity, glucose metabolism, and mitochondrial function. Functional enrichment analysis of ploidy‐specific gene sets identified that biological processes associated with metabolism, stress tolerance, and immune function were overrepresented within triploids across stress treatments. Our results suggest that triploidy impacts the transcriptional regulation of key processes that underly the stress response of Pacific oysters, resulting in downstream shifts in physiological tolerance limits that may increase susceptibility to extreme climate events that present multiple environmental stressors. The impact of chromosome set manipulation on the climate resilience of marine organisms has important implications for domestic food security within future climate scenarios, especially as triploidy induction becomes an increasingly popular tool ... Article in Journal/Newspaper Crassostrea gigas Wiley Online Library Pacific Global Change Biology 29 24 6969 6987 |
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Abstract Polyploidy has been suggested to negatively impact environmental stress tolerance, resulting in increased susceptibility to extreme climate events. In this study, we compared the genomic and physiological response of diploid (2 n ) and triploid (3 n ) Pacific oysters ( Crassostrea gigas ) to conditions present during an atmospheric heatwave that impacted the Pacific Northwestern region of the United States in the summer of 2021. Climate stressors were applied either singly (single stressor; elevated seawater temperature, 30°C) or in succession (multiple stressor; elevated seawater temperature followed by aerial emersion at 44°C), replicating conditions present within the intertidal over a tidal cycle during the event. Oyster mortality rate was elevated within stress treatments with respect to the control and was significantly higher in triploids than diploids following multiple stress exposure (36.4% vs. 14.8%). Triploids within the multiple stressor treatment exhibited signs of energetic limitation, including metabolic depression, a significant reduction in ctenidium Na + /K + ATPase activity, and the dysregulated expression of genes associated with stress response, innate immunity, glucose metabolism, and mitochondrial function. Functional enrichment analysis of ploidy‐specific gene sets identified that biological processes associated with metabolism, stress tolerance, and immune function were overrepresented within triploids across stress treatments. Our results suggest that triploidy impacts the transcriptional regulation of key processes that underly the stress response of Pacific oysters, resulting in downstream shifts in physiological tolerance limits that may increase susceptibility to extreme climate events that present multiple environmental stressors. The impact of chromosome set manipulation on the climate resilience of marine organisms has important implications for domestic food security within future climate scenarios, especially as triploidy induction becomes an increasingly popular tool ... |
format |
Article in Journal/Newspaper |
author |
George, Matthew N. Cattau, Olivia Middleton, Mollie A. Lawson, Delaney Vadopalas, Brent Gavery, Mackenzie Roberts, Steven B. |
spellingShingle |
George, Matthew N. Cattau, Olivia Middleton, Mollie A. Lawson, Delaney Vadopalas, Brent Gavery, Mackenzie Roberts, Steven B. Triploid Pacific oysters exhibit stress response dysregulation and elevated mortality following heatwaves |
author_facet |
George, Matthew N. Cattau, Olivia Middleton, Mollie A. Lawson, Delaney Vadopalas, Brent Gavery, Mackenzie Roberts, Steven B. |
author_sort |
George, Matthew N. |
title |
Triploid Pacific oysters exhibit stress response dysregulation and elevated mortality following heatwaves |
title_short |
Triploid Pacific oysters exhibit stress response dysregulation and elevated mortality following heatwaves |
title_full |
Triploid Pacific oysters exhibit stress response dysregulation and elevated mortality following heatwaves |
title_fullStr |
Triploid Pacific oysters exhibit stress response dysregulation and elevated mortality following heatwaves |
title_full_unstemmed |
Triploid Pacific oysters exhibit stress response dysregulation and elevated mortality following heatwaves |
title_sort |
triploid pacific oysters exhibit stress response dysregulation and elevated mortality following heatwaves |
publisher |
Wiley |
publishDate |
2023 |
url |
http://dx.doi.org/10.1111/gcb.16880 https://onlinelibrary.wiley.com/doi/pdf/10.1111/gcb.16880 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Global Change Biology volume 29, issue 24, page 6969-6987 ISSN 1354-1013 1365-2486 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/gcb.16880 |
container_title |
Global Change Biology |
container_volume |
29 |
container_issue |
24 |
container_start_page |
6969 |
op_container_end_page |
6987 |
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1813447389425434624 |