Effects of multiple drivers of ocean global change on the physiology and functional gene expression of the coccolithophore Emiliania huxleyi
Abstract Ongoing ocean global change due to anthropogenic activities is causing multiple chemical and physical seawater properties to change simultaneously, which may affect the physiology of marine phytoplankton. The coccolithophore Emiliania huxleyi is a model species often employed in the study o...
Published in: | Global Change Biology |
---|---|
Main Authors: | , , , , , , |
Other Authors: | |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2020
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1111/gcb.15259 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fgcb.15259 https://onlinelibrary.wiley.com/doi/pdf/10.1111/gcb.15259 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/gcb.15259 |
id |
crwiley:10.1111/gcb.15259 |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1111/gcb.15259 2024-06-23T07:55:54+00:00 Effects of multiple drivers of ocean global change on the physiology and functional gene expression of the coccolithophore Emiliania huxleyi Feng, Yuanyuan Roleda, Michael Y. Armstrong, Evelyn Summerfield, Tina C. Law, Cliff S. Hurd, Catriona L. Boyd, Philip W. Natural Science Foundation of Tianjin City Natural Science Foundation of Tianjin City Natural Science Foundation of Tianjin City 2020 http://dx.doi.org/10.1111/gcb.15259 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fgcb.15259 https://onlinelibrary.wiley.com/doi/pdf/10.1111/gcb.15259 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/gcb.15259 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Global Change Biology volume 26, issue 10, page 5630-5645 ISSN 1354-1013 1365-2486 journal-article 2020 crwiley https://doi.org/10.1111/gcb.15259 2024-06-06T04:24:01Z Abstract Ongoing ocean global change due to anthropogenic activities is causing multiple chemical and physical seawater properties to change simultaneously, which may affect the physiology of marine phytoplankton. The coccolithophore Emiliania huxleyi is a model species often employed in the study of the marine carbon cycle. The effect of ocean acidification (OA) on coccolithophore calcification has been extensively studied; however, physiological responses to multiple environmental drivers are still largely unknown. Here we examined two‐way and multiple driver effects of OA and other key environmental drivers—nitrate, phosphate, irradiance, and temperature—on the growth, photosynthetic, and calcification rates, and the elemental composition of E. huxleyi . In addition, changes in functional gene expression were examined to understand the molecular mechanisms underpinning the physiological responses. The single driver manipulation experiments suggest decreased nitrate supply being the most important driver regulating E. huxleyi physiology, by significantly reducing the growth, photosynthetic, and calcification rates. In addition, the interaction of OA and decreased nitrate supply (projected for year 2100) had more negative synergistic effects on E. huxleyi physiology than all other two‐way factorial manipulations, suggesting a linkage between the single dominant driver (nitrate) effects and interactive effects with other drivers. Simultaneous manipulation of all five environmental drivers to the conditions of the projected year 2100 had the largest negative effects on most of the physiological metrics. Furthermore, functional genes associated with inorganic carbon acquisition ( RubisCO , AEL1 , and δCA ) and calcification ( CAX3 , AEL1 , PATP , and NhaA2 ) were most downregulated by the multiple driver manipulation, revealing linkages between responses of functional gene expression and associated physiological metrics. These findings together indicate that for more holistic projections of coccolithophore ... Article in Journal/Newspaper Ocean acidification Wiley Online Library Global Change Biology 26 10 5630 5645 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Abstract Ongoing ocean global change due to anthropogenic activities is causing multiple chemical and physical seawater properties to change simultaneously, which may affect the physiology of marine phytoplankton. The coccolithophore Emiliania huxleyi is a model species often employed in the study of the marine carbon cycle. The effect of ocean acidification (OA) on coccolithophore calcification has been extensively studied; however, physiological responses to multiple environmental drivers are still largely unknown. Here we examined two‐way and multiple driver effects of OA and other key environmental drivers—nitrate, phosphate, irradiance, and temperature—on the growth, photosynthetic, and calcification rates, and the elemental composition of E. huxleyi . In addition, changes in functional gene expression were examined to understand the molecular mechanisms underpinning the physiological responses. The single driver manipulation experiments suggest decreased nitrate supply being the most important driver regulating E. huxleyi physiology, by significantly reducing the growth, photosynthetic, and calcification rates. In addition, the interaction of OA and decreased nitrate supply (projected for year 2100) had more negative synergistic effects on E. huxleyi physiology than all other two‐way factorial manipulations, suggesting a linkage between the single dominant driver (nitrate) effects and interactive effects with other drivers. Simultaneous manipulation of all five environmental drivers to the conditions of the projected year 2100 had the largest negative effects on most of the physiological metrics. Furthermore, functional genes associated with inorganic carbon acquisition ( RubisCO , AEL1 , and δCA ) and calcification ( CAX3 , AEL1 , PATP , and NhaA2 ) were most downregulated by the multiple driver manipulation, revealing linkages between responses of functional gene expression and associated physiological metrics. These findings together indicate that for more holistic projections of coccolithophore ... |
author2 |
Natural Science Foundation of Tianjin City Natural Science Foundation of Tianjin City Natural Science Foundation of Tianjin City |
format |
Article in Journal/Newspaper |
author |
Feng, Yuanyuan Roleda, Michael Y. Armstrong, Evelyn Summerfield, Tina C. Law, Cliff S. Hurd, Catriona L. Boyd, Philip W. |
spellingShingle |
Feng, Yuanyuan Roleda, Michael Y. Armstrong, Evelyn Summerfield, Tina C. Law, Cliff S. Hurd, Catriona L. Boyd, Philip W. Effects of multiple drivers of ocean global change on the physiology and functional gene expression of the coccolithophore Emiliania huxleyi |
author_facet |
Feng, Yuanyuan Roleda, Michael Y. Armstrong, Evelyn Summerfield, Tina C. Law, Cliff S. Hurd, Catriona L. Boyd, Philip W. |
author_sort |
Feng, Yuanyuan |
title |
Effects of multiple drivers of ocean global change on the physiology and functional gene expression of the coccolithophore Emiliania huxleyi |
title_short |
Effects of multiple drivers of ocean global change on the physiology and functional gene expression of the coccolithophore Emiliania huxleyi |
title_full |
Effects of multiple drivers of ocean global change on the physiology and functional gene expression of the coccolithophore Emiliania huxleyi |
title_fullStr |
Effects of multiple drivers of ocean global change on the physiology and functional gene expression of the coccolithophore Emiliania huxleyi |
title_full_unstemmed |
Effects of multiple drivers of ocean global change on the physiology and functional gene expression of the coccolithophore Emiliania huxleyi |
title_sort |
effects of multiple drivers of ocean global change on the physiology and functional gene expression of the coccolithophore emiliania huxleyi |
publisher |
Wiley |
publishDate |
2020 |
url |
http://dx.doi.org/10.1111/gcb.15259 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fgcb.15259 https://onlinelibrary.wiley.com/doi/pdf/10.1111/gcb.15259 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/gcb.15259 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Global Change Biology volume 26, issue 10, page 5630-5645 ISSN 1354-1013 1365-2486 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/gcb.15259 |
container_title |
Global Change Biology |
container_volume |
26 |
container_issue |
10 |
container_start_page |
5630 |
op_container_end_page |
5645 |
_version_ |
1802648691002048512 |