Coralline algae elevate pH at the site of calcification under ocean acidification

• Published in: Global Change Biology
• Main Authors: Cornwall, Christopher E., Comeau, Steeve, McCulloch, Malcolm T.
• Other Authors: ARC Centre of Excellence for Coral Reef Studies, ARC Laureate Fellowship Award, ARC Discovery Early Career Researcher Award
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2017
• Subjects: Ocean acidification
• Online Access: https://doi.org/10.1111/gcb.13673; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fgcb.13673; https://onlinelibrary.wiley.com/doi/pdf/10.1111/gcb.13673

Abstract Coralline algae provide important ecosystem services but are susceptible to the impacts of ocean acidification. However, the mechanisms are uncertain, and the magnitude is species specific. Here, we assess whether species‐specific responses to ocean acidification of coralline algae are related to differences in pH at the site of calcification within the calcifying fluid/medium ( pH cf ) using δ 11 B as a proxy. Declines in δ 11 B for all three species are consistent with shifts in δ 11 B expected if B( OH ) 4 − was incorporated during precipitation. In particular, the δ 11 B ratio in Amphiroa anceps was too low to allow for reasonable pH cf values if B( OH ) 3 rather than B( OH ) 4 − was directly incorporated from the calcifying fluid. This points towards δ 11 B being a reliable proxy for pH cf for coralline algal calcite and that if B( OH ) 3 is present in detectable proportions, it can be attributed to secondary postincorporation transformation of B( OH ) 4 − . We thus show that pH cf is elevated during calcification and that the extent is species specific. The net calcification of two species of coralline algae ( Sporolithon durum , and Amphiroa anceps ) declined under elevated CO 2 , as did their pH cf . Neogoniolithon sp. had the highest pH cf , and most constant calcification rates, with the decrease in pH cf being ¼ that of seawater pH in the treatments, demonstrating a control of coralline algae on carbonate chemistry at their site of calcification. The discovery that coralline algae upregulate pH cf under ocean acidification is physiologically important and should be included in future models involving calcification.