Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors
Abstract The metamorphosis of planktonic larvae of the Pacific oyster ( Crassostrea gigas ) underpins their complex life‐history strategy by switching on the molecular machinery required for sessile life and building calcite shells. Metamorphosis becomes a survival bottleneck, which will be pressure...
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crwiley:10.1111/gcb.13249 2024-06-02T08:05:37+00:00 Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors Dineshram, Ramadoss Chandramouli, Kondethimmanahalli Ko, Ginger Wai Kuen Zhang, Huoming Qian, Pei‐Yuan Ravasi, Timothy Thiyagarajan, Vengatesen Glaucoma Research Foundation 2016 http://dx.doi.org/10.1111/gcb.13249 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fgcb.13249 https://onlinelibrary.wiley.com/doi/pdf/10.1111/gcb.13249 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Global Change Biology volume 22, issue 6, page 2054-2068 ISSN 1354-1013 1365-2486 journal-article 2016 crwiley https://doi.org/10.1111/gcb.13249 2024-05-03T11:02:25Z Abstract The metamorphosis of planktonic larvae of the Pacific oyster ( Crassostrea gigas ) underpins their complex life‐history strategy by switching on the molecular machinery required for sessile life and building calcite shells. Metamorphosis becomes a survival bottleneck, which will be pressured by different anthropogenically induced climate change‐related variables. Therefore, it is important to understand how metamorphosing larvae interact with emerging climate change stressors. To predict how larvae might be affected in a future ocean, we examined changes in the proteome of metamorphosing larvae under multiple stressors: decreased pH ( pH 7.4), increased temperature (30 °C), and reduced salinity (15 psu). Quantitative protein expression profiling using iTRAQ ‐ LC ‐ MS / MS identified more than 1300 proteins. Decreased pH had a negative effect on metamorphosis by down‐regulating several proteins involved in energy production, metabolism, and protein synthesis. However, warming switched on these down‐regulated pathways at pH 7.4. Under multiple stressors, cell signaling, energy production, growth, and developmental pathways were up‐regulated, although metamorphosis was still reduced. Despite the lack of lethal effects, significant physiological responses to both individual and interacting climate change related stressors were observed at proteome level. The metamorphosing larvae of the C. gigas population in the Yellow Sea appear to have adequate phenotypic plasticity at the proteome level to survive in future coastal oceans, but with developmental and physiological costs. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Wiley Online Library Pacific Global Change Biology 22 6 2054 2068 |
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Open Polar |
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Wiley Online Library |
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English |
description |
Abstract The metamorphosis of planktonic larvae of the Pacific oyster ( Crassostrea gigas ) underpins their complex life‐history strategy by switching on the molecular machinery required for sessile life and building calcite shells. Metamorphosis becomes a survival bottleneck, which will be pressured by different anthropogenically induced climate change‐related variables. Therefore, it is important to understand how metamorphosing larvae interact with emerging climate change stressors. To predict how larvae might be affected in a future ocean, we examined changes in the proteome of metamorphosing larvae under multiple stressors: decreased pH ( pH 7.4), increased temperature (30 °C), and reduced salinity (15 psu). Quantitative protein expression profiling using iTRAQ ‐ LC ‐ MS / MS identified more than 1300 proteins. Decreased pH had a negative effect on metamorphosis by down‐regulating several proteins involved in energy production, metabolism, and protein synthesis. However, warming switched on these down‐regulated pathways at pH 7.4. Under multiple stressors, cell signaling, energy production, growth, and developmental pathways were up‐regulated, although metamorphosis was still reduced. Despite the lack of lethal effects, significant physiological responses to both individual and interacting climate change related stressors were observed at proteome level. The metamorphosing larvae of the C. gigas population in the Yellow Sea appear to have adequate phenotypic plasticity at the proteome level to survive in future coastal oceans, but with developmental and physiological costs. |
author2 |
Glaucoma Research Foundation |
format |
Article in Journal/Newspaper |
author |
Dineshram, Ramadoss Chandramouli, Kondethimmanahalli Ko, Ginger Wai Kuen Zhang, Huoming Qian, Pei‐Yuan Ravasi, Timothy Thiyagarajan, Vengatesen |
spellingShingle |
Dineshram, Ramadoss Chandramouli, Kondethimmanahalli Ko, Ginger Wai Kuen Zhang, Huoming Qian, Pei‐Yuan Ravasi, Timothy Thiyagarajan, Vengatesen Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors |
author_facet |
Dineshram, Ramadoss Chandramouli, Kondethimmanahalli Ko, Ginger Wai Kuen Zhang, Huoming Qian, Pei‐Yuan Ravasi, Timothy Thiyagarajan, Vengatesen |
author_sort |
Dineshram, Ramadoss |
title |
Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors |
title_short |
Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors |
title_full |
Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors |
title_fullStr |
Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors |
title_full_unstemmed |
Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors |
title_sort |
quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors |
publisher |
Wiley |
publishDate |
2016 |
url |
http://dx.doi.org/10.1111/gcb.13249 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fgcb.13249 https://onlinelibrary.wiley.com/doi/pdf/10.1111/gcb.13249 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Global Change Biology volume 22, issue 6, page 2054-2068 ISSN 1354-1013 1365-2486 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/gcb.13249 |
container_title |
Global Change Biology |
container_volume |
22 |
container_issue |
6 |
container_start_page |
2054 |
op_container_end_page |
2068 |
_version_ |
1800750486888906752 |