Diversity decoupled from sulfur isotope fractionation in a sulfate‐reducing microbial community
Abstract The extent of fractionation of sulfur isotopes by sulfate‐reducing microbes is dictated by genomic and environmental factors. A greater understanding of species‐specific fractionations may better inform interpretation of sulfur isotopes preserved in the rock record. To examine whether gene...
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crwiley:10.1111/gbi.12356 2024-06-02T08:02:28+00:00 Diversity decoupled from sulfur isotope fractionation in a sulfate‐reducing microbial community Colangelo‐Lillis, Jesse Pelikan, Claus Herbold, Craig W. Altshuler, Ianina Loy, Alexander Whyte, Lyle G. Wing, Boswell A. Austrian Science Fund University of Colorado Boulder 2019 http://dx.doi.org/10.1111/gbi.12356 https://onlinelibrary.wiley.com/doi/pdf/10.1111/gbi.12356 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/gbi.12356 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Geobiology volume 17, issue 6, page 660-675 ISSN 1472-4677 1472-4669 journal-article 2019 crwiley https://doi.org/10.1111/gbi.12356 2024-05-03T11:12:34Z Abstract The extent of fractionation of sulfur isotopes by sulfate‐reducing microbes is dictated by genomic and environmental factors. A greater understanding of species‐specific fractionations may better inform interpretation of sulfur isotopes preserved in the rock record. To examine whether gene diversity influences net isotopic fractionation in situ, we assessed environmental chemistry, sulfate reduction rates, diversity of putative sulfur‐metabolizing organisms by 16S rRNA and dissimilatory sulfite reductase ( dsrB ) gene amplicon sequencing, and net fractionation of sulfur isotopes along a sediment transect of a hypersaline Arctic spring. In situ sulfate reduction rates yielded minimum cell‐specific sulfate reduction rates < 0.3 × 10 −15 moles cell −1 day −1 . Neither 16S rRNA nor dsrB diversity indices correlated with relatively constant (38‰–45‰) net isotope fractionation (ε 34 S sulfide‐sulfate ). Measured ε 34 S values could be reproduced in a mechanistic fractionation model if 1%–2% of the microbial community (10%–60% of Deltaproteobacteria) were engaged in sulfate respiration, indicating heterogeneous respiratory activity within sulfate‐reducing populations. This model indicated enzymatic kinetic diversity of Apr was more likely to correlate with sulfur fractionation than DsrB. We propose that, above a threshold Shannon diversity value of 0.8 for dsrB , the influence of the specific composition of the microbial community responsible for generating an isotope signal is overprinted by the control exerted by environmental variables on microbial physiology. Article in Journal/Newspaper Arctic Wiley Online Library Arctic Geobiology 17 6 660 675 |
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English |
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Abstract The extent of fractionation of sulfur isotopes by sulfate‐reducing microbes is dictated by genomic and environmental factors. A greater understanding of species‐specific fractionations may better inform interpretation of sulfur isotopes preserved in the rock record. To examine whether gene diversity influences net isotopic fractionation in situ, we assessed environmental chemistry, sulfate reduction rates, diversity of putative sulfur‐metabolizing organisms by 16S rRNA and dissimilatory sulfite reductase ( dsrB ) gene amplicon sequencing, and net fractionation of sulfur isotopes along a sediment transect of a hypersaline Arctic spring. In situ sulfate reduction rates yielded minimum cell‐specific sulfate reduction rates < 0.3 × 10 −15 moles cell −1 day −1 . Neither 16S rRNA nor dsrB diversity indices correlated with relatively constant (38‰–45‰) net isotope fractionation (ε 34 S sulfide‐sulfate ). Measured ε 34 S values could be reproduced in a mechanistic fractionation model if 1%–2% of the microbial community (10%–60% of Deltaproteobacteria) were engaged in sulfate respiration, indicating heterogeneous respiratory activity within sulfate‐reducing populations. This model indicated enzymatic kinetic diversity of Apr was more likely to correlate with sulfur fractionation than DsrB. We propose that, above a threshold Shannon diversity value of 0.8 for dsrB , the influence of the specific composition of the microbial community responsible for generating an isotope signal is overprinted by the control exerted by environmental variables on microbial physiology. |
author2 |
Austrian Science Fund University of Colorado Boulder |
format |
Article in Journal/Newspaper |
author |
Colangelo‐Lillis, Jesse Pelikan, Claus Herbold, Craig W. Altshuler, Ianina Loy, Alexander Whyte, Lyle G. Wing, Boswell A. |
spellingShingle |
Colangelo‐Lillis, Jesse Pelikan, Claus Herbold, Craig W. Altshuler, Ianina Loy, Alexander Whyte, Lyle G. Wing, Boswell A. Diversity decoupled from sulfur isotope fractionation in a sulfate‐reducing microbial community |
author_facet |
Colangelo‐Lillis, Jesse Pelikan, Claus Herbold, Craig W. Altshuler, Ianina Loy, Alexander Whyte, Lyle G. Wing, Boswell A. |
author_sort |
Colangelo‐Lillis, Jesse |
title |
Diversity decoupled from sulfur isotope fractionation in a sulfate‐reducing microbial community |
title_short |
Diversity decoupled from sulfur isotope fractionation in a sulfate‐reducing microbial community |
title_full |
Diversity decoupled from sulfur isotope fractionation in a sulfate‐reducing microbial community |
title_fullStr |
Diversity decoupled from sulfur isotope fractionation in a sulfate‐reducing microbial community |
title_full_unstemmed |
Diversity decoupled from sulfur isotope fractionation in a sulfate‐reducing microbial community |
title_sort |
diversity decoupled from sulfur isotope fractionation in a sulfate‐reducing microbial community |
publisher |
Wiley |
publishDate |
2019 |
url |
http://dx.doi.org/10.1111/gbi.12356 https://onlinelibrary.wiley.com/doi/pdf/10.1111/gbi.12356 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/gbi.12356 |
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Arctic |
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Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Geobiology volume 17, issue 6, page 660-675 ISSN 1472-4677 1472-4669 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/gbi.12356 |
container_title |
Geobiology |
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17 |
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6 |
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660 |
op_container_end_page |
675 |
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1800746958954954752 |