A single genomic region involving a putative chromosome rearrangement in flat oyster ( Ostrea edulis) is associated with differential host resilience to the parasite Bonamia ostreae
Abstract European flat oyster ( Ostrea edulis ) is an ecologically and economically important marine bivalve, that has been severely affected by the intracellular parasite Bonamia ostreae . In this study, a flat oyster SNP array (~14,000 SNPs) was used to validate previously reported outlier loci fo...
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Online Access: | http://dx.doi.org/10.1111/eva.13446 https://onlinelibrary.wiley.com/doi/pdf/10.1111/eva.13446 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/eva.13446 |
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crwiley:10.1111/eva.13446 2024-06-02T08:11:58+00:00 A single genomic region involving a putative chromosome rearrangement in flat oyster ( Ostrea edulis) is associated with differential host resilience to the parasite Bonamia ostreae Sambade, Inés Martínez Casanova, Adrian Blanco, Andrés Gundappa, Manu K. Bean, Tim P. Macqueen, Daniel J. Houston, Ross D. Villalba, Antonio Vera, Manuel Kamermans, Pauline Martínez, Paulino Biotechnology and Biological Sciences Research Council 2022 http://dx.doi.org/10.1111/eva.13446 https://onlinelibrary.wiley.com/doi/pdf/10.1111/eva.13446 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/eva.13446 en eng Wiley http://creativecommons.org/licenses/by/4.0/ Evolutionary Applications volume 15, issue 9, page 1408-1422 ISSN 1752-4571 1752-4571 journal-article 2022 crwiley https://doi.org/10.1111/eva.13446 2024-05-03T11:23:00Z Abstract European flat oyster ( Ostrea edulis ) is an ecologically and economically important marine bivalve, that has been severely affected by the intracellular parasite Bonamia ostreae . In this study, a flat oyster SNP array (~14,000 SNPs) was used to validate previously reported outlier loci for divergent selection associated with B. ostreae exposure in the Northeast Atlantic Area. A total of 134 wild and hatchery individuals from the North Sea, collected in naïve (NV) and long‐term affected (LTA) areas, were analysed. Genetic diversity and differentiation were related to the sampling origin (wild vs. hatchery) when using neutral markers, and to bonamiosis status (NV vs. LTA) when using outlier loci for divergent selection. Two genetic clusters appeared intermingled in all sampling locations when using outlier loci, and their frequency was associated with their bonamiosis status. When both clusters were compared, outlier data sets showed high genetic divergence ( F ST > 0.25) unlike neutral loci ( F ST not ≠ 0). Moreover, the cluster associated with LTA samples showed much higher genetic diversity and significant heterozygote excess with outlier loci, but not with neutral data. Most outliers mapped on chromosome 8 (OE‐C8) of the flat oyster genome, supporting a main genomic region underlying resilience to bonamiosis. Furthermore, differentially expressed genes previously reported between NV and LTA strains showed higher mapping density on OE‐C8. A range of relevant immune functions were specifically enriched among genes annotated on OE‐C8, providing hypotheses for resilience mechanisms to an intracellular parasite. The results suggest that marker‐assisted selection could be applied to breed resilient strains of O. edulis to bonamiosis, if lower parasite load and/or higher viability of the LTA genetic cluster following B . ostreae infection is demonstrated. Article in Journal/Newspaper Northeast Atlantic Wiley Online Library Evolutionary Applications 15 9 1408 1422 |
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Wiley Online Library |
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crwiley |
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English |
description |
Abstract European flat oyster ( Ostrea edulis ) is an ecologically and economically important marine bivalve, that has been severely affected by the intracellular parasite Bonamia ostreae . In this study, a flat oyster SNP array (~14,000 SNPs) was used to validate previously reported outlier loci for divergent selection associated with B. ostreae exposure in the Northeast Atlantic Area. A total of 134 wild and hatchery individuals from the North Sea, collected in naïve (NV) and long‐term affected (LTA) areas, were analysed. Genetic diversity and differentiation were related to the sampling origin (wild vs. hatchery) when using neutral markers, and to bonamiosis status (NV vs. LTA) when using outlier loci for divergent selection. Two genetic clusters appeared intermingled in all sampling locations when using outlier loci, and their frequency was associated with their bonamiosis status. When both clusters were compared, outlier data sets showed high genetic divergence ( F ST > 0.25) unlike neutral loci ( F ST not ≠ 0). Moreover, the cluster associated with LTA samples showed much higher genetic diversity and significant heterozygote excess with outlier loci, but not with neutral data. Most outliers mapped on chromosome 8 (OE‐C8) of the flat oyster genome, supporting a main genomic region underlying resilience to bonamiosis. Furthermore, differentially expressed genes previously reported between NV and LTA strains showed higher mapping density on OE‐C8. A range of relevant immune functions were specifically enriched among genes annotated on OE‐C8, providing hypotheses for resilience mechanisms to an intracellular parasite. The results suggest that marker‐assisted selection could be applied to breed resilient strains of O. edulis to bonamiosis, if lower parasite load and/or higher viability of the LTA genetic cluster following B . ostreae infection is demonstrated. |
author2 |
Biotechnology and Biological Sciences Research Council |
format |
Article in Journal/Newspaper |
author |
Sambade, Inés Martínez Casanova, Adrian Blanco, Andrés Gundappa, Manu K. Bean, Tim P. Macqueen, Daniel J. Houston, Ross D. Villalba, Antonio Vera, Manuel Kamermans, Pauline Martínez, Paulino |
spellingShingle |
Sambade, Inés Martínez Casanova, Adrian Blanco, Andrés Gundappa, Manu K. Bean, Tim P. Macqueen, Daniel J. Houston, Ross D. Villalba, Antonio Vera, Manuel Kamermans, Pauline Martínez, Paulino A single genomic region involving a putative chromosome rearrangement in flat oyster ( Ostrea edulis) is associated with differential host resilience to the parasite Bonamia ostreae |
author_facet |
Sambade, Inés Martínez Casanova, Adrian Blanco, Andrés Gundappa, Manu K. Bean, Tim P. Macqueen, Daniel J. Houston, Ross D. Villalba, Antonio Vera, Manuel Kamermans, Pauline Martínez, Paulino |
author_sort |
Sambade, Inés Martínez |
title |
A single genomic region involving a putative chromosome rearrangement in flat oyster ( Ostrea edulis) is associated with differential host resilience to the parasite Bonamia ostreae |
title_short |
A single genomic region involving a putative chromosome rearrangement in flat oyster ( Ostrea edulis) is associated with differential host resilience to the parasite Bonamia ostreae |
title_full |
A single genomic region involving a putative chromosome rearrangement in flat oyster ( Ostrea edulis) is associated with differential host resilience to the parasite Bonamia ostreae |
title_fullStr |
A single genomic region involving a putative chromosome rearrangement in flat oyster ( Ostrea edulis) is associated with differential host resilience to the parasite Bonamia ostreae |
title_full_unstemmed |
A single genomic region involving a putative chromosome rearrangement in flat oyster ( Ostrea edulis) is associated with differential host resilience to the parasite Bonamia ostreae |
title_sort |
single genomic region involving a putative chromosome rearrangement in flat oyster ( ostrea edulis) is associated with differential host resilience to the parasite bonamia ostreae |
publisher |
Wiley |
publishDate |
2022 |
url |
http://dx.doi.org/10.1111/eva.13446 https://onlinelibrary.wiley.com/doi/pdf/10.1111/eva.13446 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/eva.13446 |
genre |
Northeast Atlantic |
genre_facet |
Northeast Atlantic |
op_source |
Evolutionary Applications volume 15, issue 9, page 1408-1422 ISSN 1752-4571 1752-4571 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1111/eva.13446 |
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Evolutionary Applications |
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15 |
container_issue |
9 |
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1408 |
op_container_end_page |
1422 |
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1800758261056536576 |