Selection on oxidative phosphorylation and ribosomal structure as a multigenerational response to ocean acidification in the common copepod Pseudocalanus acuspes
Abstract Ocean acidification is expected to have dramatic impacts on oceanic ecosystems, yet surprisingly few studies currently examine long‐term adaptive and plastic responses of marine invertebrates to p CO 2 stress. Here, we exposed populations of the common copepod Pseudocalanus acuspes to three...
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crwiley:10.1111/eva.12335 2024-06-02T08:12:31+00:00 Selection on oxidative phosphorylation and ribosomal structure as a multigenerational response to ocean acidification in the common copepod Pseudocalanus acuspes De Wit, Pierre Dupont, Sam Thor, Peter Vetenskapsrådet Norges Forskningsråd 2015 http://dx.doi.org/10.1111/eva.12335 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Feva.12335 https://onlinelibrary.wiley.com/doi/pdf/10.1111/eva.12335 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/eva.12335 en eng Wiley http://creativecommons.org/licenses/by/4.0/ Evolutionary Applications volume 9, issue 9, page 1112-1123 ISSN 1752-4571 1752-4571 journal-article 2015 crwiley https://doi.org/10.1111/eva.12335 2024-05-03T10:55:25Z Abstract Ocean acidification is expected to have dramatic impacts on oceanic ecosystems, yet surprisingly few studies currently examine long‐term adaptive and plastic responses of marine invertebrates to p CO 2 stress. Here, we exposed populations of the common copepod Pseudocalanus acuspes to three p CO 2 regimes (400, 900, and 1550 μatm) for two generations, after which we conducted a reciprocal transplant experiment. A de novo transcriptome was assembled, annotated, and gene expression data revealed that genes involved in RNA transcription were strongly down‐regulated in populations with long‐term exposure to a high p CO 2 environment, even after transplantation back to control levels. In addition, 747 000 SNP s were identified, out of which 1513 showed consistent changes in nucleotide frequency between replicates of control and high p CO 2 populations. Functions involving RNA transcription and ribosomal function, as well as ion transport and oxidative phosphorylation, were highly overrepresented. We thus conclude that p CO 2 stress appears to impose selection in copepods on RNA synthesis and translation, possibly modulated by helicase expression. Using a physiological hypothesis‐testing strategy to mine gene expression data, we herein increase the power to detect cellular targets of ocean acidification. This novel approach seems promising for future studies of effects of environmental changes in ecologically important nonmodel organisms. Article in Journal/Newspaper Ocean acidification Copepods Wiley Online Library Evolutionary Applications 9 9 1112 1123 |
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Wiley Online Library |
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crwiley |
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English |
description |
Abstract Ocean acidification is expected to have dramatic impacts on oceanic ecosystems, yet surprisingly few studies currently examine long‐term adaptive and plastic responses of marine invertebrates to p CO 2 stress. Here, we exposed populations of the common copepod Pseudocalanus acuspes to three p CO 2 regimes (400, 900, and 1550 μatm) for two generations, after which we conducted a reciprocal transplant experiment. A de novo transcriptome was assembled, annotated, and gene expression data revealed that genes involved in RNA transcription were strongly down‐regulated in populations with long‐term exposure to a high p CO 2 environment, even after transplantation back to control levels. In addition, 747 000 SNP s were identified, out of which 1513 showed consistent changes in nucleotide frequency between replicates of control and high p CO 2 populations. Functions involving RNA transcription and ribosomal function, as well as ion transport and oxidative phosphorylation, were highly overrepresented. We thus conclude that p CO 2 stress appears to impose selection in copepods on RNA synthesis and translation, possibly modulated by helicase expression. Using a physiological hypothesis‐testing strategy to mine gene expression data, we herein increase the power to detect cellular targets of ocean acidification. This novel approach seems promising for future studies of effects of environmental changes in ecologically important nonmodel organisms. |
author2 |
Vetenskapsrådet Norges Forskningsråd |
format |
Article in Journal/Newspaper |
author |
De Wit, Pierre Dupont, Sam Thor, Peter |
spellingShingle |
De Wit, Pierre Dupont, Sam Thor, Peter Selection on oxidative phosphorylation and ribosomal structure as a multigenerational response to ocean acidification in the common copepod Pseudocalanus acuspes |
author_facet |
De Wit, Pierre Dupont, Sam Thor, Peter |
author_sort |
De Wit, Pierre |
title |
Selection on oxidative phosphorylation and ribosomal structure as a multigenerational response to ocean acidification in the common copepod Pseudocalanus acuspes |
title_short |
Selection on oxidative phosphorylation and ribosomal structure as a multigenerational response to ocean acidification in the common copepod Pseudocalanus acuspes |
title_full |
Selection on oxidative phosphorylation and ribosomal structure as a multigenerational response to ocean acidification in the common copepod Pseudocalanus acuspes |
title_fullStr |
Selection on oxidative phosphorylation and ribosomal structure as a multigenerational response to ocean acidification in the common copepod Pseudocalanus acuspes |
title_full_unstemmed |
Selection on oxidative phosphorylation and ribosomal structure as a multigenerational response to ocean acidification in the common copepod Pseudocalanus acuspes |
title_sort |
selection on oxidative phosphorylation and ribosomal structure as a multigenerational response to ocean acidification in the common copepod pseudocalanus acuspes |
publisher |
Wiley |
publishDate |
2015 |
url |
http://dx.doi.org/10.1111/eva.12335 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Feva.12335 https://onlinelibrary.wiley.com/doi/pdf/10.1111/eva.12335 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/eva.12335 |
genre |
Ocean acidification Copepods |
genre_facet |
Ocean acidification Copepods |
op_source |
Evolutionary Applications volume 9, issue 9, page 1112-1123 ISSN 1752-4571 1752-4571 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1111/eva.12335 |
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Evolutionary Applications |
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9 |
container_issue |
9 |
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1112 |
op_container_end_page |
1123 |
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1800758954696900608 |