First evidence of Wolbachia infection in populations of grasshopper Podisma sapporensis (Orthoptera: Acrididae)

Abstract The brachypterous grasshopper Podisma sapporensis (Orthoptera: Acrididae) is distributed throughout the Sakhalin, Kunashir and Hokkaido Islands. Karyotypes of this species consist of two major chromosomal races with different sex chromosome systems, XO/XX and XY/XX. Molecular phylogeographi...

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Bibliographic Details
Published in:Entomological Science
Main Authors: Bugrov, Alexander G., Ilinsky, Yury Yu., Strunov, Anton, Zhukova, Mariya, Kiseleva, Elena, Akimoto, Shin‐ichi, Tatsuta, Haruki
Other Authors: OPTEC grant for young scientists and JSPS KAKENHI
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 2016
Subjects:
Sakhalin
Online Access:http://dx.doi.org/10.1111/ens.12187
https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fens.12187
https://onlinelibrary.wiley.com/doi/pdf/10.1111/ens.12187
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Summary:Abstract The brachypterous grasshopper Podisma sapporensis (Orthoptera: Acrididae) is distributed throughout the Sakhalin, Kunashir and Hokkaido Islands. Karyotypes of this species consist of two major chromosomal races with different sex chromosome systems, XO/XX and XY/XX. Molecular phylogeographic analysis of the chromosome races and subraces confirms the genetic divergence of the races and subraces in P. sapporensis . Here we first report that P. sapporensis is infected with Wolbachia consisting of three variants on wsp locus, while gatB locus was monomorphic. Furthermore, observation of cell tissue of P. sapporensis using electron microscopy confirmed the infection of Wolbachia that was inferred from polymerase chain reaction and revealed the distribution of the bacteria in the head, thorax and abdomen of P. sapporensis embryos. Our finding may shed new light on Wolbachia as a possible agent causing hybrid dysfunction resulting from experimental crosses between chromosome races or subraces of P. sapporensis .

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