Copper resistance in the cold: Genome analysis and characterisation of a P IB‐1 ATPase in Bizionia argentinensis
Abstract Copper homeostasis is a fundamental process in organisms, characterised by unique pathways that have evolved to meet specific needs while preserving core resistance mechanisms. While these systems are well‐documented in model bacteria, information on copper resistance in species adapted to...
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crwiley:10.1111/1758-2229.13278 2024-09-15T17:42:29+00:00 Copper resistance in the cold: Genome analysis and characterisation of a P IB‐1 ATPase in Bizionia argentinensis Burgardt, Noelia I. Melian, Noelia A. González Flecha, F. Luis Fondo para la Investigación Científica y Tecnológica Consejo Nacional de Investigaciones Científicas y Técnicas Universidad de Buenos Aires 2024 http://dx.doi.org/10.1111/1758-2229.13278 en eng Wiley http://creativecommons.org/licenses/by/4.0/ Environmental Microbiology Reports volume 16, issue 4 ISSN 1758-2229 1758-2229 journal-article 2024 crwiley https://doi.org/10.1111/1758-2229.13278 2024-08-30T04:13:08Z Abstract Copper homeostasis is a fundamental process in organisms, characterised by unique pathways that have evolved to meet specific needs while preserving core resistance mechanisms. While these systems are well‐documented in model bacteria, information on copper resistance in species adapted to cold environments is scarce. This study investigates the potential genes related to copper homeostasis in the genome of Bizionia argentinensis (JUB59‐T), a psychrotolerant bacterium isolated from Antarctic seawater. We identified several genes encoding proteins analogous to those crucial for copper homeostasis, including three sequences of copper‐transport P1B‐type ATPases. One of these, referred to as BaCopA1, was chosen for cloning and expression in Saccharomyces cerevisiae . BaCopA1 was successfully integrated into yeast membranes and subsequently extracted with detergent. The purified BaCopA1 demonstrated the ability to catalyse ATP hydrolysis at low temperatures. Structural models of various BaCopA1 conformations were generated and compared with mesophilic and thermophilic homologous structures. The significant conservation of critical residues and structural similarity among these proteins suggest a shared reaction mechanism for copper transport. This study is the first to report a psychrotolerant P1B‐ATPase that has been expressed and purified in a functional form. Article in Journal/Newspaper Antarc* Antarctic Wiley Online Library Environmental Microbiology Reports 16 4 |
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Wiley Online Library |
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English |
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Abstract Copper homeostasis is a fundamental process in organisms, characterised by unique pathways that have evolved to meet specific needs while preserving core resistance mechanisms. While these systems are well‐documented in model bacteria, information on copper resistance in species adapted to cold environments is scarce. This study investigates the potential genes related to copper homeostasis in the genome of Bizionia argentinensis (JUB59‐T), a psychrotolerant bacterium isolated from Antarctic seawater. We identified several genes encoding proteins analogous to those crucial for copper homeostasis, including three sequences of copper‐transport P1B‐type ATPases. One of these, referred to as BaCopA1, was chosen for cloning and expression in Saccharomyces cerevisiae . BaCopA1 was successfully integrated into yeast membranes and subsequently extracted with detergent. The purified BaCopA1 demonstrated the ability to catalyse ATP hydrolysis at low temperatures. Structural models of various BaCopA1 conformations were generated and compared with mesophilic and thermophilic homologous structures. The significant conservation of critical residues and structural similarity among these proteins suggest a shared reaction mechanism for copper transport. This study is the first to report a psychrotolerant P1B‐ATPase that has been expressed and purified in a functional form. |
author2 |
Fondo para la Investigación Científica y Tecnológica Consejo Nacional de Investigaciones Científicas y Técnicas Universidad de Buenos Aires |
format |
Article in Journal/Newspaper |
author |
Burgardt, Noelia I. Melian, Noelia A. González Flecha, F. Luis |
spellingShingle |
Burgardt, Noelia I. Melian, Noelia A. González Flecha, F. Luis Copper resistance in the cold: Genome analysis and characterisation of a P IB‐1 ATPase in Bizionia argentinensis |
author_facet |
Burgardt, Noelia I. Melian, Noelia A. González Flecha, F. Luis |
author_sort |
Burgardt, Noelia I. |
title |
Copper resistance in the cold: Genome analysis and characterisation of a P IB‐1 ATPase in Bizionia argentinensis |
title_short |
Copper resistance in the cold: Genome analysis and characterisation of a P IB‐1 ATPase in Bizionia argentinensis |
title_full |
Copper resistance in the cold: Genome analysis and characterisation of a P IB‐1 ATPase in Bizionia argentinensis |
title_fullStr |
Copper resistance in the cold: Genome analysis and characterisation of a P IB‐1 ATPase in Bizionia argentinensis |
title_full_unstemmed |
Copper resistance in the cold: Genome analysis and characterisation of a P IB‐1 ATPase in Bizionia argentinensis |
title_sort |
copper resistance in the cold: genome analysis and characterisation of a p ib‐1 atpase in bizionia argentinensis |
publisher |
Wiley |
publishDate |
2024 |
url |
http://dx.doi.org/10.1111/1758-2229.13278 |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Environmental Microbiology Reports volume 16, issue 4 ISSN 1758-2229 1758-2229 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1111/1758-2229.13278 |
container_title |
Environmental Microbiology Reports |
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16 |
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4 |
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1810489071623995392 |