The effect of protracted X‐ray exposure on cell survival and metabolic activity of fast and slow growing fungi capable of melanogenesis
Summary The aim of this study was to analyse how protracted exposure to X‐rays delivered at low dose rates of 0.0032–0.052 kGy h −1 affects the survival and metabolic activity of two microfungi capable of melanogenesis: fast‐growing Cryptococcus neoformans (CN) and slow‐growing Cryomyces antarcticus...
Published in: | Environmental Microbiology Reports |
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Online Access: | http://dx.doi.org/10.1111/1758-2229.12632 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1758-2229.12632 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1758-2229.12632 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1758-2229.12632 https://sfamjournals.onlinelibrary.wiley.com/doi/am-pdf/10.1111/1758-2229.12632 |
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crwiley:10.1111/1758-2229.12632 2024-09-15T17:44:34+00:00 The effect of protracted X‐ray exposure on cell survival and metabolic activity of fast and slow growing fungi capable of melanogenesis Pacelli, Claudia Bryan, Ruth A. Onofri, Silvano Selbmann, Laura Zucconi, Laura Shuryak, Igor Dadachova, Ekaterina Defense Threat Reduction Agency Agenzia Spaziale Italiana 2018 http://dx.doi.org/10.1111/1758-2229.12632 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1758-2229.12632 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1758-2229.12632 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1758-2229.12632 https://sfamjournals.onlinelibrary.wiley.com/doi/am-pdf/10.1111/1758-2229.12632 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#am http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Microbiology Reports volume 10, issue 3, page 255-263 ISSN 1758-2229 1758-2229 journal-article 2018 crwiley https://doi.org/10.1111/1758-2229.12632 2024-08-30T04:13:05Z Summary The aim of this study was to analyse how protracted exposure to X‐rays delivered at low dose rates of 0.0032–0.052 kGy h −1 affects the survival and metabolic activity of two microfungi capable of melanogenesis: fast‐growing Cryptococcus neoformans (CN) and slow‐growing Cryomyces antarcticus (CA). Melanized CN and CA cells survived the protracted exposure better than non‐melanized ones, which was consistent with previous reports on the radioprotective role of melanin in these fungi after high dose rate exposures. The survival data were described by the linear quadratic dose response model. The XTT metabolic profiles were practically identical for melanized CN and CA with activity dose‐dependent increasing: no changes in the activity of the non‐melanized CN and CA were recorded by this assay. In contrast, the MTT assay, which measures the intracellular energy‐related processes, recorded an increase in activity of non‐melanized CN and CA cells, but not in their melanized counterparts. This could reflect intensive repair processes initiated by the non‐melanized cells post exposure. This study suggests that differences in radiation responses between melanized and non‐melanized fungal cells occur over a wide range of radiation dose rates. Article in Journal/Newspaper Antarc* antarcticus Wiley Online Library Environmental Microbiology Reports 10 3 255 263 |
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Wiley Online Library |
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English |
description |
Summary The aim of this study was to analyse how protracted exposure to X‐rays delivered at low dose rates of 0.0032–0.052 kGy h −1 affects the survival and metabolic activity of two microfungi capable of melanogenesis: fast‐growing Cryptococcus neoformans (CN) and slow‐growing Cryomyces antarcticus (CA). Melanized CN and CA cells survived the protracted exposure better than non‐melanized ones, which was consistent with previous reports on the radioprotective role of melanin in these fungi after high dose rate exposures. The survival data were described by the linear quadratic dose response model. The XTT metabolic profiles were practically identical for melanized CN and CA with activity dose‐dependent increasing: no changes in the activity of the non‐melanized CN and CA were recorded by this assay. In contrast, the MTT assay, which measures the intracellular energy‐related processes, recorded an increase in activity of non‐melanized CN and CA cells, but not in their melanized counterparts. This could reflect intensive repair processes initiated by the non‐melanized cells post exposure. This study suggests that differences in radiation responses between melanized and non‐melanized fungal cells occur over a wide range of radiation dose rates. |
author2 |
Defense Threat Reduction Agency Agenzia Spaziale Italiana |
format |
Article in Journal/Newspaper |
author |
Pacelli, Claudia Bryan, Ruth A. Onofri, Silvano Selbmann, Laura Zucconi, Laura Shuryak, Igor Dadachova, Ekaterina |
spellingShingle |
Pacelli, Claudia Bryan, Ruth A. Onofri, Silvano Selbmann, Laura Zucconi, Laura Shuryak, Igor Dadachova, Ekaterina The effect of protracted X‐ray exposure on cell survival and metabolic activity of fast and slow growing fungi capable of melanogenesis |
author_facet |
Pacelli, Claudia Bryan, Ruth A. Onofri, Silvano Selbmann, Laura Zucconi, Laura Shuryak, Igor Dadachova, Ekaterina |
author_sort |
Pacelli, Claudia |
title |
The effect of protracted X‐ray exposure on cell survival and metabolic activity of fast and slow growing fungi capable of melanogenesis |
title_short |
The effect of protracted X‐ray exposure on cell survival and metabolic activity of fast and slow growing fungi capable of melanogenesis |
title_full |
The effect of protracted X‐ray exposure on cell survival and metabolic activity of fast and slow growing fungi capable of melanogenesis |
title_fullStr |
The effect of protracted X‐ray exposure on cell survival and metabolic activity of fast and slow growing fungi capable of melanogenesis |
title_full_unstemmed |
The effect of protracted X‐ray exposure on cell survival and metabolic activity of fast and slow growing fungi capable of melanogenesis |
title_sort |
effect of protracted x‐ray exposure on cell survival and metabolic activity of fast and slow growing fungi capable of melanogenesis |
publisher |
Wiley |
publishDate |
2018 |
url |
http://dx.doi.org/10.1111/1758-2229.12632 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1758-2229.12632 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1758-2229.12632 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1758-2229.12632 https://sfamjournals.onlinelibrary.wiley.com/doi/am-pdf/10.1111/1758-2229.12632 |
genre |
Antarc* antarcticus |
genre_facet |
Antarc* antarcticus |
op_source |
Environmental Microbiology Reports volume 10, issue 3, page 255-263 ISSN 1758-2229 1758-2229 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#am http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/1758-2229.12632 |
container_title |
Environmental Microbiology Reports |
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10 |
container_issue |
3 |
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255 |
op_container_end_page |
263 |
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1810492209290543104 |