Nitrate addition has minimal short‐term impacts on Greenland ice sheet supraglacial prokaryotes
Summary Tropospheric nitrate levels are predicted to increase throughout the 21 st century, with potential effects on terrestrial ecosystems, including the Greenland ice sheet (GrIS). This study considers the impacts of elevated nitrate concentrations on the abundance and composition of dominant bul...
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crwiley:10.1111/1758-2229.12510 2024-06-02T08:07:26+00:00 Nitrate addition has minimal short‐term impacts on Greenland ice sheet supraglacial prokaryotes Cameron, Karen A. Stibal, Marek Chrismas, Nathan Box, Jason Jacobsen, Carsten S. Villum Young Investigator Programme Danish Research Council 2017 http://dx.doi.org/10.1111/1758-2229.12510 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1758-2229.12510 http://onlinelibrary.wiley.com/wol1/doi/10.1111/1758-2229.12510/fullpdf en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Microbiology Reports volume 9, issue 2, page 144-150 ISSN 1758-2229 1758-2229 journal-article 2017 crwiley https://doi.org/10.1111/1758-2229.12510 2024-05-03T12:01:00Z Summary Tropospheric nitrate levels are predicted to increase throughout the 21 st century, with potential effects on terrestrial ecosystems, including the Greenland ice sheet (GrIS). This study considers the impacts of elevated nitrate concentrations on the abundance and composition of dominant bulk and active prokaryotic communities sampled from in situ nitrate fertilization plots on the GrIS surface. Nitrate concentrations were successfully elevated within sediment‐filled meltwater pools, known as cryoconite holes; however, nitrate additions applied to surface ice did not persist. Estimated bulk and active cryoconite community cell abundance was unaltered by nitrate additions when compared to control holes using a quantitative PCR approach, and nitrate was found to have a minimal affect on the dominant 16S rRNA gene‐based community composition. Together, these results indicate that sampled cryoconite communities were not nitrate limited at the time of sampling. Instead, temporal changes in biomass and community composition were more pronounced. As these in situ incubations were short (6 weeks), and the community composition across GrIS surface ice is highly variable, we suggest that further efforts should be considered to investigate the potential long‐term impacts of increased nitrate across the GrIS. Article in Journal/Newspaper Greenland Ice Sheet Wiley Online Library Greenland Environmental Microbiology Reports 9 2 144 150 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Summary Tropospheric nitrate levels are predicted to increase throughout the 21 st century, with potential effects on terrestrial ecosystems, including the Greenland ice sheet (GrIS). This study considers the impacts of elevated nitrate concentrations on the abundance and composition of dominant bulk and active prokaryotic communities sampled from in situ nitrate fertilization plots on the GrIS surface. Nitrate concentrations were successfully elevated within sediment‐filled meltwater pools, known as cryoconite holes; however, nitrate additions applied to surface ice did not persist. Estimated bulk and active cryoconite community cell abundance was unaltered by nitrate additions when compared to control holes using a quantitative PCR approach, and nitrate was found to have a minimal affect on the dominant 16S rRNA gene‐based community composition. Together, these results indicate that sampled cryoconite communities were not nitrate limited at the time of sampling. Instead, temporal changes in biomass and community composition were more pronounced. As these in situ incubations were short (6 weeks), and the community composition across GrIS surface ice is highly variable, we suggest that further efforts should be considered to investigate the potential long‐term impacts of increased nitrate across the GrIS. |
author2 |
Villum Young Investigator Programme Danish Research Council |
format |
Article in Journal/Newspaper |
author |
Cameron, Karen A. Stibal, Marek Chrismas, Nathan Box, Jason Jacobsen, Carsten S. |
spellingShingle |
Cameron, Karen A. Stibal, Marek Chrismas, Nathan Box, Jason Jacobsen, Carsten S. Nitrate addition has minimal short‐term impacts on Greenland ice sheet supraglacial prokaryotes |
author_facet |
Cameron, Karen A. Stibal, Marek Chrismas, Nathan Box, Jason Jacobsen, Carsten S. |
author_sort |
Cameron, Karen A. |
title |
Nitrate addition has minimal short‐term impacts on Greenland ice sheet supraglacial prokaryotes |
title_short |
Nitrate addition has minimal short‐term impacts on Greenland ice sheet supraglacial prokaryotes |
title_full |
Nitrate addition has minimal short‐term impacts on Greenland ice sheet supraglacial prokaryotes |
title_fullStr |
Nitrate addition has minimal short‐term impacts on Greenland ice sheet supraglacial prokaryotes |
title_full_unstemmed |
Nitrate addition has minimal short‐term impacts on Greenland ice sheet supraglacial prokaryotes |
title_sort |
nitrate addition has minimal short‐term impacts on greenland ice sheet supraglacial prokaryotes |
publisher |
Wiley |
publishDate |
2017 |
url |
http://dx.doi.org/10.1111/1758-2229.12510 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1758-2229.12510 http://onlinelibrary.wiley.com/wol1/doi/10.1111/1758-2229.12510/fullpdf |
geographic |
Greenland |
geographic_facet |
Greenland |
genre |
Greenland Ice Sheet |
genre_facet |
Greenland Ice Sheet |
op_source |
Environmental Microbiology Reports volume 9, issue 2, page 144-150 ISSN 1758-2229 1758-2229 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/1758-2229.12510 |
container_title |
Environmental Microbiology Reports |
container_volume |
9 |
container_issue |
2 |
container_start_page |
144 |
op_container_end_page |
150 |
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1800752512179896320 |