The transcriptional regulation of the glyoxylate cycle in SAR11 in response to iron fertilization in the Southern Ocean
Summary The tricarboxylic acid ( TCA ) cycle is a central metabolic pathway that is present in all aerobic organisms and initiates the respiration of organic material. The glyoxylate cycle is a variation of the TCA cycle, where organic material is recycled for subsequent assimilation into cell mater...
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crwiley:10.1111/1758-2229.12267 2024-09-15T18:37:16+00:00 The transcriptional regulation of the glyoxylate cycle in SAR11 in response to iron fertilization in the Southern Ocean Beier, Sara Gálvez, María J. Molina, Veronica Sarthou, Géraldine Quéroué, Fabien Blain, Stephane Obernosterer, Ingrid Agence Nationale de la Recherche CONICYT-PCHA Ecos-sud 2015 http://dx.doi.org/10.1111/1758-2229.12267 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1758-2229.12267 http://onlinelibrary.wiley.com/wol1/doi/10.1111/1758-2229.12267/fullpdf en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Microbiology Reports volume 7, issue 3, page 427-434 ISSN 1758-2229 1758-2229 journal-article 2015 crwiley https://doi.org/10.1111/1758-2229.12267 2024-08-06T04:18:16Z Summary The tricarboxylic acid ( TCA ) cycle is a central metabolic pathway that is present in all aerobic organisms and initiates the respiration of organic material. The glyoxylate cycle is a variation of the TCA cycle, where organic material is recycled for subsequent assimilation into cell material instead of being released as carbon dioxide. Despite the importance for the fate of organic matter, the environmental factors that induce the glyoxylate cycle in microbial communities remain poorly understood. In this study, we assessed the expression of isocitrate lyase, the enzyme that induces the switch to the glyoxylate cycle, of the ubiquitous SAR 11 clade in response to natural iron fertilization in the S outhern O cean. The cell‐specific transcriptional regulation of the glyoxylate cycle, as determined by the ratio between copy numbers of isocitrate lyase gene transcripts and isocitrate genes, was consistently lower in iron fertilized than in high‐nutrient, low chlorophyll waters (by 2.4‐ to 16.5‐fold). SAR 11 cell‐specific isocitrate lyase gene transcription was negatively correlated to chlorophyll a , and bulk bacterial heterotrophic metabolism. We conclude that the glyoxylate cycle is a metabolic strategy for SAR 11 that is highly sensitive to the degree of iron and carbon limitation in the marine environment. Article in Journal/Newspaper Southern Ocean Wiley Online Library Environmental Microbiology Reports 7 3 427 434 |
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Wiley Online Library |
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English |
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Summary The tricarboxylic acid ( TCA ) cycle is a central metabolic pathway that is present in all aerobic organisms and initiates the respiration of organic material. The glyoxylate cycle is a variation of the TCA cycle, where organic material is recycled for subsequent assimilation into cell material instead of being released as carbon dioxide. Despite the importance for the fate of organic matter, the environmental factors that induce the glyoxylate cycle in microbial communities remain poorly understood. In this study, we assessed the expression of isocitrate lyase, the enzyme that induces the switch to the glyoxylate cycle, of the ubiquitous SAR 11 clade in response to natural iron fertilization in the S outhern O cean. The cell‐specific transcriptional regulation of the glyoxylate cycle, as determined by the ratio between copy numbers of isocitrate lyase gene transcripts and isocitrate genes, was consistently lower in iron fertilized than in high‐nutrient, low chlorophyll waters (by 2.4‐ to 16.5‐fold). SAR 11 cell‐specific isocitrate lyase gene transcription was negatively correlated to chlorophyll a , and bulk bacterial heterotrophic metabolism. We conclude that the glyoxylate cycle is a metabolic strategy for SAR 11 that is highly sensitive to the degree of iron and carbon limitation in the marine environment. |
author2 |
Agence Nationale de la Recherche CONICYT-PCHA Ecos-sud |
format |
Article in Journal/Newspaper |
author |
Beier, Sara Gálvez, María J. Molina, Veronica Sarthou, Géraldine Quéroué, Fabien Blain, Stephane Obernosterer, Ingrid |
spellingShingle |
Beier, Sara Gálvez, María J. Molina, Veronica Sarthou, Géraldine Quéroué, Fabien Blain, Stephane Obernosterer, Ingrid The transcriptional regulation of the glyoxylate cycle in SAR11 in response to iron fertilization in the Southern Ocean |
author_facet |
Beier, Sara Gálvez, María J. Molina, Veronica Sarthou, Géraldine Quéroué, Fabien Blain, Stephane Obernosterer, Ingrid |
author_sort |
Beier, Sara |
title |
The transcriptional regulation of the glyoxylate cycle in SAR11 in response to iron fertilization in the Southern Ocean |
title_short |
The transcriptional regulation of the glyoxylate cycle in SAR11 in response to iron fertilization in the Southern Ocean |
title_full |
The transcriptional regulation of the glyoxylate cycle in SAR11 in response to iron fertilization in the Southern Ocean |
title_fullStr |
The transcriptional regulation of the glyoxylate cycle in SAR11 in response to iron fertilization in the Southern Ocean |
title_full_unstemmed |
The transcriptional regulation of the glyoxylate cycle in SAR11 in response to iron fertilization in the Southern Ocean |
title_sort |
transcriptional regulation of the glyoxylate cycle in sar11 in response to iron fertilization in the southern ocean |
publisher |
Wiley |
publishDate |
2015 |
url |
http://dx.doi.org/10.1111/1758-2229.12267 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1758-2229.12267 http://onlinelibrary.wiley.com/wol1/doi/10.1111/1758-2229.12267/fullpdf |
genre |
Southern Ocean |
genre_facet |
Southern Ocean |
op_source |
Environmental Microbiology Reports volume 7, issue 3, page 427-434 ISSN 1758-2229 1758-2229 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/1758-2229.12267 |
container_title |
Environmental Microbiology Reports |
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7 |
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3 |
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427 |
op_container_end_page |
434 |
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1810481618959204352 |