Different bulk and active bacterial communities in cryoconite from the margin and interior of the Greenland ice sheet
Summary Biological processes in the supraglacial ecosystem, including cryoconite, contribute to nutrient cycling within the cryosphere and may affect surface melting, yet little is known of the diversity of the active microbes in these environments. We examined the bacterial abundance and community...
Published in: | Environmental Microbiology Reports |
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crwiley:10.1111/1758-2229.12246 2024-06-23T07:53:21+00:00 Different bulk and active bacterial communities in cryoconite from the margin and interior of the Greenland ice sheet Stibal, Marek Schostag, Morten Cameron, Karen A. Hansen, Lars H. Chandler, David M. Wadham, Jemma L. Jacobsen, Carsten S. Villum Foundation 2015 http://dx.doi.org/10.1111/1758-2229.12246 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1758-2229.12246 http://onlinelibrary.wiley.com/wol1/doi/10.1111/1758-2229.12246/fullpdf en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Microbiology Reports volume 7, issue 2, page 293-300 ISSN 1758-2229 1758-2229 journal-article 2015 crwiley https://doi.org/10.1111/1758-2229.12246 2024-06-13T04:23:54Z Summary Biological processes in the supraglacial ecosystem, including cryoconite, contribute to nutrient cycling within the cryosphere and may affect surface melting, yet little is known of the diversity of the active microbes in these environments. We examined the bacterial abundance and community composition of cryoconite over a melt season at two contrasting sites at the margin and in the interior of the G reenland ice sheet, using sequence analysis and quantitative polymerase chain reaction of coextracted 16S rDNA and rRNA . Significant differences were found between bulk ( rDNA ) and potentially active ( rRNA ) communities, and between communities sampled from the two sites. Higher concentrations of rRNA than rDNA were detected at the interior site, whereas at the margin several orders of magnitude less rRNA was found compared with rDNA , which may be explained by a lower proportion of active bacteria at the margin site. The rRNA communities at both sites were dominated by a few taxa of Cyanobacteria and Alpha‐ and/or Betaproteobacteria. The bulk alpha diversity was higher in the margin site community, suggesting that local sources may be contributing towards the gene pool in addition to long distance transport. Article in Journal/Newspaper Greenland Ice Sheet Wiley Online Library Greenland Environmental Microbiology Reports 7 2 293 300 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Summary Biological processes in the supraglacial ecosystem, including cryoconite, contribute to nutrient cycling within the cryosphere and may affect surface melting, yet little is known of the diversity of the active microbes in these environments. We examined the bacterial abundance and community composition of cryoconite over a melt season at two contrasting sites at the margin and in the interior of the G reenland ice sheet, using sequence analysis and quantitative polymerase chain reaction of coextracted 16S rDNA and rRNA . Significant differences were found between bulk ( rDNA ) and potentially active ( rRNA ) communities, and between communities sampled from the two sites. Higher concentrations of rRNA than rDNA were detected at the interior site, whereas at the margin several orders of magnitude less rRNA was found compared with rDNA , which may be explained by a lower proportion of active bacteria at the margin site. The rRNA communities at both sites were dominated by a few taxa of Cyanobacteria and Alpha‐ and/or Betaproteobacteria. The bulk alpha diversity was higher in the margin site community, suggesting that local sources may be contributing towards the gene pool in addition to long distance transport. |
author2 |
Villum Foundation |
format |
Article in Journal/Newspaper |
author |
Stibal, Marek Schostag, Morten Cameron, Karen A. Hansen, Lars H. Chandler, David M. Wadham, Jemma L. Jacobsen, Carsten S. |
spellingShingle |
Stibal, Marek Schostag, Morten Cameron, Karen A. Hansen, Lars H. Chandler, David M. Wadham, Jemma L. Jacobsen, Carsten S. Different bulk and active bacterial communities in cryoconite from the margin and interior of the Greenland ice sheet |
author_facet |
Stibal, Marek Schostag, Morten Cameron, Karen A. Hansen, Lars H. Chandler, David M. Wadham, Jemma L. Jacobsen, Carsten S. |
author_sort |
Stibal, Marek |
title |
Different bulk and active bacterial communities in cryoconite from the margin and interior of the Greenland ice sheet |
title_short |
Different bulk and active bacterial communities in cryoconite from the margin and interior of the Greenland ice sheet |
title_full |
Different bulk and active bacterial communities in cryoconite from the margin and interior of the Greenland ice sheet |
title_fullStr |
Different bulk and active bacterial communities in cryoconite from the margin and interior of the Greenland ice sheet |
title_full_unstemmed |
Different bulk and active bacterial communities in cryoconite from the margin and interior of the Greenland ice sheet |
title_sort |
different bulk and active bacterial communities in cryoconite from the margin and interior of the greenland ice sheet |
publisher |
Wiley |
publishDate |
2015 |
url |
http://dx.doi.org/10.1111/1758-2229.12246 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1758-2229.12246 http://onlinelibrary.wiley.com/wol1/doi/10.1111/1758-2229.12246/fullpdf |
geographic |
Greenland |
geographic_facet |
Greenland |
genre |
Greenland Ice Sheet |
genre_facet |
Greenland Ice Sheet |
op_source |
Environmental Microbiology Reports volume 7, issue 2, page 293-300 ISSN 1758-2229 1758-2229 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/1758-2229.12246 |
container_title |
Environmental Microbiology Reports |
container_volume |
7 |
container_issue |
2 |
container_start_page |
293 |
op_container_end_page |
300 |
_version_ |
1802644954317586432 |