Selective occurrence of Rhizobiales in frost flowers on the surface of young sea ice near Barrow, Alaska and distribution in the polar marine rare biosphere
Summary Frost flowers are highly saline ice structures that grow on the surface of young sea ice, a spatially extensive environment of increasing importance in the A rctic O cean. In a previous study, we reported organic components of frost flowers in the form of elevated levels of bacteria and exop...
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crwiley:10.1111/1758-2229.12047 2024-06-02T08:04:12+00:00 Selective occurrence of Rhizobiales in frost flowers on the surface of young sea ice near Barrow, Alaska and distribution in the polar marine rare biosphere Bowman, J. S. Larose, C. Vogel, T. M. Deming, J. W. 2013 http://dx.doi.org/10.1111/1758-2229.12047 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1758-2229.12047 http://onlinelibrary.wiley.com/wol1/doi/10.1111/1758-2229.12047/fullpdf en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Microbiology Reports volume 5, issue 4, page 575-582 ISSN 1758-2229 1758-2229 journal-article 2013 crwiley https://doi.org/10.1111/1758-2229.12047 2024-05-03T11:30:33Z Summary Frost flowers are highly saline ice structures that grow on the surface of young sea ice, a spatially extensive environment of increasing importance in the A rctic O cean. In a previous study, we reported organic components of frost flowers in the form of elevated levels of bacteria and exopolymers relative to underlying ice. Here, DNA was extracted from frost flowers and young sea ice, collected in springtime from a frozen lead offshore of B arrow, A laska, to identify bacteria in these understudied environments. Evaluation of the distribution of 16S rRNA genes via four methods (microarray analysis, T ‐ RFLP , clone library and shotgun metagenomic sequencing) indicated distinctive bacterial assemblages between the two environments, with frost flowers appearing to select for R hizobiales . A phylogenetic placement approach, used to evaluate the distribution of similar R hizobiales sequences in other polar marine studies, indicated that some of the observed strains represent widely distributed members of the marine rare biosphere in both the A rctic and A ntarctic. Article in Journal/Newspaper Barrow Sea ice Alaska Wiley Online Library Environmental Microbiology Reports 5 4 575 582 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Summary Frost flowers are highly saline ice structures that grow on the surface of young sea ice, a spatially extensive environment of increasing importance in the A rctic O cean. In a previous study, we reported organic components of frost flowers in the form of elevated levels of bacteria and exopolymers relative to underlying ice. Here, DNA was extracted from frost flowers and young sea ice, collected in springtime from a frozen lead offshore of B arrow, A laska, to identify bacteria in these understudied environments. Evaluation of the distribution of 16S rRNA genes via four methods (microarray analysis, T ‐ RFLP , clone library and shotgun metagenomic sequencing) indicated distinctive bacterial assemblages between the two environments, with frost flowers appearing to select for R hizobiales . A phylogenetic placement approach, used to evaluate the distribution of similar R hizobiales sequences in other polar marine studies, indicated that some of the observed strains represent widely distributed members of the marine rare biosphere in both the A rctic and A ntarctic. |
format |
Article in Journal/Newspaper |
author |
Bowman, J. S. Larose, C. Vogel, T. M. Deming, J. W. |
spellingShingle |
Bowman, J. S. Larose, C. Vogel, T. M. Deming, J. W. Selective occurrence of Rhizobiales in frost flowers on the surface of young sea ice near Barrow, Alaska and distribution in the polar marine rare biosphere |
author_facet |
Bowman, J. S. Larose, C. Vogel, T. M. Deming, J. W. |
author_sort |
Bowman, J. S. |
title |
Selective occurrence of Rhizobiales in frost flowers on the surface of young sea ice near Barrow, Alaska and distribution in the polar marine rare biosphere |
title_short |
Selective occurrence of Rhizobiales in frost flowers on the surface of young sea ice near Barrow, Alaska and distribution in the polar marine rare biosphere |
title_full |
Selective occurrence of Rhizobiales in frost flowers on the surface of young sea ice near Barrow, Alaska and distribution in the polar marine rare biosphere |
title_fullStr |
Selective occurrence of Rhizobiales in frost flowers on the surface of young sea ice near Barrow, Alaska and distribution in the polar marine rare biosphere |
title_full_unstemmed |
Selective occurrence of Rhizobiales in frost flowers on the surface of young sea ice near Barrow, Alaska and distribution in the polar marine rare biosphere |
title_sort |
selective occurrence of rhizobiales in frost flowers on the surface of young sea ice near barrow, alaska and distribution in the polar marine rare biosphere |
publisher |
Wiley |
publishDate |
2013 |
url |
http://dx.doi.org/10.1111/1758-2229.12047 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1758-2229.12047 http://onlinelibrary.wiley.com/wol1/doi/10.1111/1758-2229.12047/fullpdf |
genre |
Barrow Sea ice Alaska |
genre_facet |
Barrow Sea ice Alaska |
op_source |
Environmental Microbiology Reports volume 5, issue 4, page 575-582 ISSN 1758-2229 1758-2229 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/1758-2229.12047 |
container_title |
Environmental Microbiology Reports |
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5 |
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4 |
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575 |
op_container_end_page |
582 |
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1800748837833277440 |