A reliable DNA barcode reference library for the identification of the North European shelf fish fauna
Abstract Valid fish species identification is an essential step both for fundamental science and fisheries management. The traditional identification is mainly based on external morphological diagnostic characters, leading to inconsistent results in many cases. Here, we provide a sequence reference...
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crwiley:10.1111/1755-0998.12238 2024-09-15T18:23:39+00:00 A reliable DNA barcode reference library for the identification of the North European shelf fish fauna Knebelsberger, Thomas Landi, Monica Neumann, Hermann Kloppmann, Matthias Sell, Anne F. Campbell, Patrick D. Laakmann, Silke Raupach, Michael J. Carvalho, Gary R. Costa, Filipe O. 2014 http://dx.doi.org/10.1111/1755-0998.12238 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1755-0998.12238 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1755-0998.12238 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Molecular Ecology Resources volume 14, issue 5, page 1060-1071 ISSN 1755-098X 1755-0998 journal-article 2014 crwiley https://doi.org/10.1111/1755-0998.12238 2024-08-30T04:11:02Z Abstract Valid fish species identification is an essential step both for fundamental science and fisheries management. The traditional identification is mainly based on external morphological diagnostic characters, leading to inconsistent results in many cases. Here, we provide a sequence reference library based on mitochondrial cytochrome c oxidase subunit I ( COI ) for a valid identification of 93 North Atlantic fish species originating from the North Sea and adjacent waters, including many commercially exploited species. Neighbour‐joining analysis based on K2P genetic distances formed nonoverlapping clusters for all species with a ≥99% bootstrap support each. Identification was successful for 100% of the species as the minimum genetic distance to the nearest neighbour always exceeded the maximum intraspecific distance. A barcoding gap was apparent for the whole data set. Within‐species distances ranged from 0 to 2.35%, while interspecific distances varied between 3.15 and 28.09%. Distances between congeners were on average 51‐fold higher than those within species. The validation of the sequence library by applying BOLD s barcode index number ( BIN ) analysis tool and a ranking system demonstrated high taxonomic reliability of the DNA barcodes for 85% of the investigated fish species. Thus, the sequence library presented here can be confidently used as a benchmark for identification of at least two‐thirds of the typical fish species recorded for the North Sea. Article in Journal/Newspaper North Atlantic Wiley Online Library Molecular Ecology Resources 14 5 1060 1071 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Valid fish species identification is an essential step both for fundamental science and fisheries management. The traditional identification is mainly based on external morphological diagnostic characters, leading to inconsistent results in many cases. Here, we provide a sequence reference library based on mitochondrial cytochrome c oxidase subunit I ( COI ) for a valid identification of 93 North Atlantic fish species originating from the North Sea and adjacent waters, including many commercially exploited species. Neighbour‐joining analysis based on K2P genetic distances formed nonoverlapping clusters for all species with a ≥99% bootstrap support each. Identification was successful for 100% of the species as the minimum genetic distance to the nearest neighbour always exceeded the maximum intraspecific distance. A barcoding gap was apparent for the whole data set. Within‐species distances ranged from 0 to 2.35%, while interspecific distances varied between 3.15 and 28.09%. Distances between congeners were on average 51‐fold higher than those within species. The validation of the sequence library by applying BOLD s barcode index number ( BIN ) analysis tool and a ranking system demonstrated high taxonomic reliability of the DNA barcodes for 85% of the investigated fish species. Thus, the sequence library presented here can be confidently used as a benchmark for identification of at least two‐thirds of the typical fish species recorded for the North Sea. |
format |
Article in Journal/Newspaper |
author |
Knebelsberger, Thomas Landi, Monica Neumann, Hermann Kloppmann, Matthias Sell, Anne F. Campbell, Patrick D. Laakmann, Silke Raupach, Michael J. Carvalho, Gary R. Costa, Filipe O. |
spellingShingle |
Knebelsberger, Thomas Landi, Monica Neumann, Hermann Kloppmann, Matthias Sell, Anne F. Campbell, Patrick D. Laakmann, Silke Raupach, Michael J. Carvalho, Gary R. Costa, Filipe O. A reliable DNA barcode reference library for the identification of the North European shelf fish fauna |
author_facet |
Knebelsberger, Thomas Landi, Monica Neumann, Hermann Kloppmann, Matthias Sell, Anne F. Campbell, Patrick D. Laakmann, Silke Raupach, Michael J. Carvalho, Gary R. Costa, Filipe O. |
author_sort |
Knebelsberger, Thomas |
title |
A reliable DNA barcode reference library for the identification of the North European shelf fish fauna |
title_short |
A reliable DNA barcode reference library for the identification of the North European shelf fish fauna |
title_full |
A reliable DNA barcode reference library for the identification of the North European shelf fish fauna |
title_fullStr |
A reliable DNA barcode reference library for the identification of the North European shelf fish fauna |
title_full_unstemmed |
A reliable DNA barcode reference library for the identification of the North European shelf fish fauna |
title_sort |
reliable dna barcode reference library for the identification of the north european shelf fish fauna |
publisher |
Wiley |
publishDate |
2014 |
url |
http://dx.doi.org/10.1111/1755-0998.12238 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1755-0998.12238 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1755-0998.12238 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Molecular Ecology Resources volume 14, issue 5, page 1060-1071 ISSN 1755-098X 1755-0998 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/1755-0998.12238 |
container_title |
Molecular Ecology Resources |
container_volume |
14 |
container_issue |
5 |
container_start_page |
1060 |
op_container_end_page |
1071 |
_version_ |
1810463890788581376 |