Effect of TDA‐producing Phaeobacter inhibens on the fish pathogen Vibrio anguillarum in non‐axenic algae and copepod systems
Summary The expanding aquaculture industry plays an important role in feeding the growing human population and with the expansion, sustainable bacterial disease control, such as probiotics, becomes increasingly important. Tropodithietic acid (TDA)‐producing Phaeobacter spp. can protect live feed, fo...
Published in: | Microbial Biotechnology |
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crwiley:10.1111/1751-7915.13275 2024-04-14T08:20:39+00:00 Effect of TDA‐producing Phaeobacter inhibens on the fish pathogen Vibrio anguillarum in non‐axenic algae and copepod systems Rasmussen, Bastian Barker Erner, Katrine Ege Bentzon‐Tilia, Mikkel Gram, Lone Strategiske Forskningsråd Villum Fonden 2018 http://dx.doi.org/10.1111/1751-7915.13275 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1751-7915.13275 http://onlinelibrary.wiley.com/wol1/doi/10.1111/1751-7915.13275/fullpdf en eng Wiley http://creativecommons.org/licenses/by/4.0/ Microbial Biotechnology volume 11, issue 6, page 1070-1079 ISSN 1751-7915 1751-7915 Applied Microbiology and Biotechnology Biochemistry Bioengineering Biotechnology journal-article 2018 crwiley https://doi.org/10.1111/1751-7915.13275 2024-03-19T10:55:10Z Summary The expanding aquaculture industry plays an important role in feeding the growing human population and with the expansion, sustainable bacterial disease control, such as probiotics, becomes increasingly important. Tropodithietic acid (TDA)‐producing Phaeobacter spp. can protect live feed, for example rotifers and Artemia as well as larvae of turbot and cod against pathogenic vibrios. Here, we show that the emerging live feed, copepods, is unaffected by colonization of the fish pathogen Vibrio anguillarum , making them potential infection vectors. However, TDA‐producing Phaeobacter inhibens was able to significantly inhibit V. anguillarum in non‐axenic cultures of copepod Acartia tonsa and the copepod feed Rhodomonas salina . Vibrio grew to 10 6 CFU ml −1 and 10 7 CFU ml −1 in copepod and R. salina cultures, respectively. However, vibrio counts remained at the inoculum level (10 4 CFU ml −1 ) when P. inhibens was also added. We further developed a semi‐strain‐specific qPCR for V. anguillarum to detect and quantify the pathogen in non‐axenic systems. In conclusion, P. inhibens efficiently inhibits the fish larval pathogen V. anguillarum in the emerging live feed, copepods, supporting its use as a probiotic in aquaculture. Furthermore, qPCR provides an effective method for detecting vibrio pathogens in complex non‐axenic live feed systems. Article in Journal/Newspaper Turbot Copepods Wiley Online Library Microbial Biotechnology 11 6 1070 1079 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
topic |
Applied Microbiology and Biotechnology Biochemistry Bioengineering Biotechnology |
spellingShingle |
Applied Microbiology and Biotechnology Biochemistry Bioengineering Biotechnology Rasmussen, Bastian Barker Erner, Katrine Ege Bentzon‐Tilia, Mikkel Gram, Lone Effect of TDA‐producing Phaeobacter inhibens on the fish pathogen Vibrio anguillarum in non‐axenic algae and copepod systems |
topic_facet |
Applied Microbiology and Biotechnology Biochemistry Bioengineering Biotechnology |
description |
Summary The expanding aquaculture industry plays an important role in feeding the growing human population and with the expansion, sustainable bacterial disease control, such as probiotics, becomes increasingly important. Tropodithietic acid (TDA)‐producing Phaeobacter spp. can protect live feed, for example rotifers and Artemia as well as larvae of turbot and cod against pathogenic vibrios. Here, we show that the emerging live feed, copepods, is unaffected by colonization of the fish pathogen Vibrio anguillarum , making them potential infection vectors. However, TDA‐producing Phaeobacter inhibens was able to significantly inhibit V. anguillarum in non‐axenic cultures of copepod Acartia tonsa and the copepod feed Rhodomonas salina . Vibrio grew to 10 6 CFU ml −1 and 10 7 CFU ml −1 in copepod and R. salina cultures, respectively. However, vibrio counts remained at the inoculum level (10 4 CFU ml −1 ) when P. inhibens was also added. We further developed a semi‐strain‐specific qPCR for V. anguillarum to detect and quantify the pathogen in non‐axenic systems. In conclusion, P. inhibens efficiently inhibits the fish larval pathogen V. anguillarum in the emerging live feed, copepods, supporting its use as a probiotic in aquaculture. Furthermore, qPCR provides an effective method for detecting vibrio pathogens in complex non‐axenic live feed systems. |
author2 |
Strategiske Forskningsråd Villum Fonden |
format |
Article in Journal/Newspaper |
author |
Rasmussen, Bastian Barker Erner, Katrine Ege Bentzon‐Tilia, Mikkel Gram, Lone |
author_facet |
Rasmussen, Bastian Barker Erner, Katrine Ege Bentzon‐Tilia, Mikkel Gram, Lone |
author_sort |
Rasmussen, Bastian Barker |
title |
Effect of TDA‐producing Phaeobacter inhibens on the fish pathogen Vibrio anguillarum in non‐axenic algae and copepod systems |
title_short |
Effect of TDA‐producing Phaeobacter inhibens on the fish pathogen Vibrio anguillarum in non‐axenic algae and copepod systems |
title_full |
Effect of TDA‐producing Phaeobacter inhibens on the fish pathogen Vibrio anguillarum in non‐axenic algae and copepod systems |
title_fullStr |
Effect of TDA‐producing Phaeobacter inhibens on the fish pathogen Vibrio anguillarum in non‐axenic algae and copepod systems |
title_full_unstemmed |
Effect of TDA‐producing Phaeobacter inhibens on the fish pathogen Vibrio anguillarum in non‐axenic algae and copepod systems |
title_sort |
effect of tda‐producing phaeobacter inhibens on the fish pathogen vibrio anguillarum in non‐axenic algae and copepod systems |
publisher |
Wiley |
publishDate |
2018 |
url |
http://dx.doi.org/10.1111/1751-7915.13275 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1751-7915.13275 http://onlinelibrary.wiley.com/wol1/doi/10.1111/1751-7915.13275/fullpdf |
genre |
Turbot Copepods |
genre_facet |
Turbot Copepods |
op_source |
Microbial Biotechnology volume 11, issue 6, page 1070-1079 ISSN 1751-7915 1751-7915 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1111/1751-7915.13275 |
container_title |
Microbial Biotechnology |
container_volume |
11 |
container_issue |
6 |
container_start_page |
1070 |
op_container_end_page |
1079 |
_version_ |
1796299000987516928 |