Calcium absorption by Alaska pollock surimi protein hydrolysate promotes osteoblast differentiation
Abstract The calcium‐binding capacity and osteoblast proliferation and differentiation were studied in Alaska pollock surimi hydrolysate (APSH) using a system that mimics the gastrointestinal digestive system. Evaluation of the calcium absorption–promoting ability of APSH revealed that the best calc...
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Online Access: | http://dx.doi.org/10.1111/1750-3841.16988 |
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crwiley:10.1111/1750-3841.16988 2024-06-02T07:54:25+00:00 Calcium absorption by Alaska pollock surimi protein hydrolysate promotes osteoblast differentiation Eom, TaeKil Nam, Taek‐Jeong 2024 http://dx.doi.org/10.1111/1750-3841.16988 en eng Wiley http://creativecommons.org/licenses/by/4.0/ Journal of Food Science volume 89, issue 4, page 2482-2493 ISSN 0022-1147 1750-3841 journal-article 2024 crwiley https://doi.org/10.1111/1750-3841.16988 2024-05-03T10:48:13Z Abstract The calcium‐binding capacity and osteoblast proliferation and differentiation were studied in Alaska pollock surimi hydrolysate (APSH) using a system that mimics the gastrointestinal digestive system. Evaluation of the calcium absorption–promoting ability of APSH revealed that the best calcium‐binding ability was achieved after hydrolysis with a combination of pepsin, α‐chymotrypsin, and trypsin, and separation into <3 kDa (APSH‐I), 3–5 kDa (APSH‐II), 5–10 kDa (APSH‐III), and <10 kDa (APSH‐IV) fractions. Scanning electron microscopy with energy‐dispersive X‐ray spectroscopy analysis confirmed that the hydrolysate and calcium ions formed a complex. Comparison of the calcium absorption capacity using Caco‐2 cells showed that calcium absorption was promoted by these hydrolysates. Measurement of the osteoblast activation revealed higher alkaline phosphatase activity, collagen synthesis, and mineralization effect for the low‐molecular‐weight hydrolysate (LMH) than for the other hydrolysates. In addition, LMH promoted the expression of osteocalcin, osteopontin, and bone morphogenetic protein‐2 and ‐4, which are hormones related to bone formation. Expression of the Runx2 transcription factor, which regulates the expression of these hormones, also increased. These results suggest that Alaska pollock surimi protein hydrolysates prepared using a system that mimics gastrointestinal hydrolysis may result in better osteoblast proliferation and bone health than those prepared using other proteases. Article in Journal/Newspaper alaska pollock Alaska Wiley Online Library Journal of Food Science 89 4 2482 2493 |
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Open Polar |
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Wiley Online Library |
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English |
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Abstract The calcium‐binding capacity and osteoblast proliferation and differentiation were studied in Alaska pollock surimi hydrolysate (APSH) using a system that mimics the gastrointestinal digestive system. Evaluation of the calcium absorption–promoting ability of APSH revealed that the best calcium‐binding ability was achieved after hydrolysis with a combination of pepsin, α‐chymotrypsin, and trypsin, and separation into <3 kDa (APSH‐I), 3–5 kDa (APSH‐II), 5–10 kDa (APSH‐III), and <10 kDa (APSH‐IV) fractions. Scanning electron microscopy with energy‐dispersive X‐ray spectroscopy analysis confirmed that the hydrolysate and calcium ions formed a complex. Comparison of the calcium absorption capacity using Caco‐2 cells showed that calcium absorption was promoted by these hydrolysates. Measurement of the osteoblast activation revealed higher alkaline phosphatase activity, collagen synthesis, and mineralization effect for the low‐molecular‐weight hydrolysate (LMH) than for the other hydrolysates. In addition, LMH promoted the expression of osteocalcin, osteopontin, and bone morphogenetic protein‐2 and ‐4, which are hormones related to bone formation. Expression of the Runx2 transcription factor, which regulates the expression of these hormones, also increased. These results suggest that Alaska pollock surimi protein hydrolysates prepared using a system that mimics gastrointestinal hydrolysis may result in better osteoblast proliferation and bone health than those prepared using other proteases. |
format |
Article in Journal/Newspaper |
author |
Eom, TaeKil Nam, Taek‐Jeong |
spellingShingle |
Eom, TaeKil Nam, Taek‐Jeong Calcium absorption by Alaska pollock surimi protein hydrolysate promotes osteoblast differentiation |
author_facet |
Eom, TaeKil Nam, Taek‐Jeong |
author_sort |
Eom, TaeKil |
title |
Calcium absorption by Alaska pollock surimi protein hydrolysate promotes osteoblast differentiation |
title_short |
Calcium absorption by Alaska pollock surimi protein hydrolysate promotes osteoblast differentiation |
title_full |
Calcium absorption by Alaska pollock surimi protein hydrolysate promotes osteoblast differentiation |
title_fullStr |
Calcium absorption by Alaska pollock surimi protein hydrolysate promotes osteoblast differentiation |
title_full_unstemmed |
Calcium absorption by Alaska pollock surimi protein hydrolysate promotes osteoblast differentiation |
title_sort |
calcium absorption by alaska pollock surimi protein hydrolysate promotes osteoblast differentiation |
publisher |
Wiley |
publishDate |
2024 |
url |
http://dx.doi.org/10.1111/1750-3841.16988 |
genre |
alaska pollock Alaska |
genre_facet |
alaska pollock Alaska |
op_source |
Journal of Food Science volume 89, issue 4, page 2482-2493 ISSN 0022-1147 1750-3841 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1111/1750-3841.16988 |
container_title |
Journal of Food Science |
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89 |
container_issue |
4 |
container_start_page |
2482 |
op_container_end_page |
2493 |
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1800759790510538752 |