Enzymatic enrichment of acylglycerols rich in n − 3 polyunsaturated fatty acids by selective methanolysis: Optimization and kinetic studies
Abstract n − 3 Polyunsaturated fatty acids ( n − 3 PUFA) have special physiological effect, but their contents in natural oils may not meet the growing demand. Lipase‐catalyzed selective methanolysis could be used to produce acylglycerols rich in n − 3 PUFA. To explore the kinetics of enzymatic meth...
Published in: | Journal of Food Science |
---|---|
Main Authors: | , , , , , |
Other Authors: | |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2023
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1111/1750-3841.16618 |
id |
crwiley:10.1111/1750-3841.16618 |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1111/1750-3841.16618 2024-09-15T17:41:47+00:00 Enzymatic enrichment of acylglycerols rich in n − 3 polyunsaturated fatty acids by selective methanolysis: Optimization and kinetic studies Jiang, Cong Wang, Zixin Huang, Ye Wang, Xiaosan Chang, Ming Wang, Xingguo National Natural Science Foundation of China 2023 http://dx.doi.org/10.1111/1750-3841.16618 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Food Science volume 88, issue 7, page 2858-2869 ISSN 0022-1147 1750-3841 journal-article 2023 crwiley https://doi.org/10.1111/1750-3841.16618 2024-07-23T04:13:04Z Abstract n − 3 Polyunsaturated fatty acids ( n − 3 PUFA) have special physiological effect, but their contents in natural oils may not meet the growing demand. Lipase‐catalyzed selective methanolysis could be used to produce acylglycerols rich in n − 3 PUFA. To explore the kinetics of enzymatic methanolysis, factors affecting the reaction, including reaction system, water content, substrate molar ratio, temperature, lipase loading, and reaction time, were first investigated in the view of optimizing the reaction. Then the effects of triacylglycerol concentrations and methanol concentrations on initial reaction rate were studied. Finally, the key kinetic parameters of methanolysis were determined subsequently. The results showed that under optimal conditions, the n − 3 PUFA content in acylglycerols increased from 39.88% to 71.41%, and the n − 3 PUFA yield was 73.67%. The reaction followed a Ping‐Pong Bi Bi mechanism with inhibition by methanol. The kinetic analysis indicated the lipase could selectively remove saturated fatty acids (SFA) and monounsaturated fatty acids (MUFA) in acylglycerols. The inhibition constant of methanol to the n − 3 PUFA (K iM , 0.30 mmol/L) was lower than that to the SFA and MUFA (219.64 and 79.71 mmol/L). The combined effects of the fatty acid selectivity of Candida antarctica lipase A and methanol inhibition resulted in an enrichment of n − 3 PUFA in acylglycerols. Overall, the methanolysis reaction catalyzed by the lipase A is a prospective enrichment method. Practical Application This study demonstrated that enzymatic selective methanolysis is a prospective enrichment method to produce acylglycerols rich in n − 3 PUFA. This method is highly efficient, environment‐friendly, and simple. n − 3 PUFA concentrates have been widely applied in the food, health‐care food, and pharmaceutical industries. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Journal of Food Science 88 7 2858 2869 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Abstract n − 3 Polyunsaturated fatty acids ( n − 3 PUFA) have special physiological effect, but their contents in natural oils may not meet the growing demand. Lipase‐catalyzed selective methanolysis could be used to produce acylglycerols rich in n − 3 PUFA. To explore the kinetics of enzymatic methanolysis, factors affecting the reaction, including reaction system, water content, substrate molar ratio, temperature, lipase loading, and reaction time, were first investigated in the view of optimizing the reaction. Then the effects of triacylglycerol concentrations and methanol concentrations on initial reaction rate were studied. Finally, the key kinetic parameters of methanolysis were determined subsequently. The results showed that under optimal conditions, the n − 3 PUFA content in acylglycerols increased from 39.88% to 71.41%, and the n − 3 PUFA yield was 73.67%. The reaction followed a Ping‐Pong Bi Bi mechanism with inhibition by methanol. The kinetic analysis indicated the lipase could selectively remove saturated fatty acids (SFA) and monounsaturated fatty acids (MUFA) in acylglycerols. The inhibition constant of methanol to the n − 3 PUFA (K iM , 0.30 mmol/L) was lower than that to the SFA and MUFA (219.64 and 79.71 mmol/L). The combined effects of the fatty acid selectivity of Candida antarctica lipase A and methanol inhibition resulted in an enrichment of n − 3 PUFA in acylglycerols. Overall, the methanolysis reaction catalyzed by the lipase A is a prospective enrichment method. Practical Application This study demonstrated that enzymatic selective methanolysis is a prospective enrichment method to produce acylglycerols rich in n − 3 PUFA. This method is highly efficient, environment‐friendly, and simple. n − 3 PUFA concentrates have been widely applied in the food, health‐care food, and pharmaceutical industries. |
author2 |
National Natural Science Foundation of China |
format |
Article in Journal/Newspaper |
author |
Jiang, Cong Wang, Zixin Huang, Ye Wang, Xiaosan Chang, Ming Wang, Xingguo |
spellingShingle |
Jiang, Cong Wang, Zixin Huang, Ye Wang, Xiaosan Chang, Ming Wang, Xingguo Enzymatic enrichment of acylglycerols rich in n − 3 polyunsaturated fatty acids by selective methanolysis: Optimization and kinetic studies |
author_facet |
Jiang, Cong Wang, Zixin Huang, Ye Wang, Xiaosan Chang, Ming Wang, Xingguo |
author_sort |
Jiang, Cong |
title |
Enzymatic enrichment of acylglycerols rich in n − 3 polyunsaturated fatty acids by selective methanolysis: Optimization and kinetic studies |
title_short |
Enzymatic enrichment of acylglycerols rich in n − 3 polyunsaturated fatty acids by selective methanolysis: Optimization and kinetic studies |
title_full |
Enzymatic enrichment of acylglycerols rich in n − 3 polyunsaturated fatty acids by selective methanolysis: Optimization and kinetic studies |
title_fullStr |
Enzymatic enrichment of acylglycerols rich in n − 3 polyunsaturated fatty acids by selective methanolysis: Optimization and kinetic studies |
title_full_unstemmed |
Enzymatic enrichment of acylglycerols rich in n − 3 polyunsaturated fatty acids by selective methanolysis: Optimization and kinetic studies |
title_sort |
enzymatic enrichment of acylglycerols rich in n − 3 polyunsaturated fatty acids by selective methanolysis: optimization and kinetic studies |
publisher |
Wiley |
publishDate |
2023 |
url |
http://dx.doi.org/10.1111/1750-3841.16618 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Journal of Food Science volume 88, issue 7, page 2858-2869 ISSN 0022-1147 1750-3841 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/1750-3841.16618 |
container_title |
Journal of Food Science |
container_volume |
88 |
container_issue |
7 |
container_start_page |
2858 |
op_container_end_page |
2869 |
_version_ |
1810488040505737216 |