Two Novel Bioactive Peptides from Antarctic Krill with Dual Angiotensin Converting Enzyme and Dipeptidyl Peptidase IV Inhibitory Activities

• Published in: Journal of Food Science
• Main Authors: Ji, Wei, Zhang, Chaohua, Ji, Hongwu
• Other Authors: Guangdong Ocean University Strong School Innovation Fund Project, Disciplinary Construction Project of Institutions of Higher Learning in Guangdong Province, Industry-University Research Cooperation Project in Guangdong Province, Modern Agro-industry Technology Research System of China
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2017
• Subjects: Antarctic; The Antarctic; Antarc*; Antarctic Krill
• Online Access: http://dx.doi.org/10.1111/1750-3841.13735; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1750-3841.13735; http://onlinelibrary.wiley.com/wol1/doi/10.1111/1750-3841.13735/fullpdf

Abstract Inhibition of dipeptidyl peptidase IV (DPP‐IV) and angiotensin converting enzyme (ACE) are considered useful in managing 2 often associated conditions: diabetes and hypertension. In this study, corolase PP was used to hydrolyze Antarctic krill protein. The hydrolysate (AKH) was isolated by ultrafiltration and purified by size‐exclusion chromatography, ion exchange chromatography and reversed‐phase high‐performance liquid chromatography (RP‐HPLC) sequentially. The in vitro inhibitory activities of all AKHs and several fractions obtained against ACE and DPP‐IV were assessed. Two peptides, purified with dual‐strength inhibitory activity against ACE and DPP‐IV, were identified by TOF‐MS/MS. Results indicated that not all fractions exhibited dual inhibitory activities of ACE and DPP‐IV. The purified peptide Lys‐Val‐Glu‐Pro‐Leu‐Pro had half‐maximal inhibitory concentrations (IC 50 ) of 0.93±0.05 and 0.73±0.04 mg/mL against ACE and DPP‐IV, respectively. The other peptide Pro‐Ala‐Leu had IC 50 values of 0.64±0.05 and 0.88±0.03 mg/mL against ACE and DPP‐IV, respectively. This study firstly reported the sequences of dual bioactive peptides from Antarctic krill proteins, further provided new insights into the bioactive peptides responsible for the ACE and DPP‐IV inhibitory activities from the Antarctic krill protein hydrolysate to manage hypertension and diabetes.