Two Novel Bioactive Peptides from Antarctic Krill with Dual Angiotensin Converting Enzyme and Dipeptidyl Peptidase IV Inhibitory Activities
Abstract Inhibition of dipeptidyl peptidase IV (DPP‐IV) and angiotensin converting enzyme (ACE) are considered useful in managing 2 often associated conditions: diabetes and hypertension. In this study, corolase PP was used to hydrolyze Antarctic krill protein. The hydrolysate (AKH) was isolated by...
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crwiley:10.1111/1750-3841.13735 2024-06-02T07:57:29+00:00 Two Novel Bioactive Peptides from Antarctic Krill with Dual Angiotensin Converting Enzyme and Dipeptidyl Peptidase IV Inhibitory Activities Ji, Wei Zhang, Chaohua Ji, Hongwu Guangdong Ocean University Strong School Innovation Fund Project Disciplinary Construction Project of Institutions of Higher Learning in Guangdong Province Industry-University Research Cooperation Project in Guangdong Province Modern Agro-industry Technology Research System of China 2017 http://dx.doi.org/10.1111/1750-3841.13735 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1750-3841.13735 http://onlinelibrary.wiley.com/wol1/doi/10.1111/1750-3841.13735/fullpdf en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Food Science volume 82, issue 7, page 1742-1749 ISSN 0022-1147 1750-3841 journal-article 2017 crwiley https://doi.org/10.1111/1750-3841.13735 2024-05-03T10:41:28Z Abstract Inhibition of dipeptidyl peptidase IV (DPP‐IV) and angiotensin converting enzyme (ACE) are considered useful in managing 2 often associated conditions: diabetes and hypertension. In this study, corolase PP was used to hydrolyze Antarctic krill protein. The hydrolysate (AKH) was isolated by ultrafiltration and purified by size‐exclusion chromatography, ion exchange chromatography and reversed‐phase high‐performance liquid chromatography (RP‐HPLC) sequentially. The in vitro inhibitory activities of all AKHs and several fractions obtained against ACE and DPP‐IV were assessed. Two peptides, purified with dual‐strength inhibitory activity against ACE and DPP‐IV, were identified by TOF‐MS/MS. Results indicated that not all fractions exhibited dual inhibitory activities of ACE and DPP‐IV. The purified peptide Lys‐Val‐Glu‐Pro‐Leu‐Pro had half‐maximal inhibitory concentrations (IC 50 ) of 0.93±0.05 and 0.73±0.04 mg/mL against ACE and DPP‐IV, respectively. The other peptide Pro‐Ala‐Leu had IC 50 values of 0.64±0.05 and 0.88±0.03 mg/mL against ACE and DPP‐IV, respectively. This study firstly reported the sequences of dual bioactive peptides from Antarctic krill proteins, further provided new insights into the bioactive peptides responsible for the ACE and DPP‐IV inhibitory activities from the Antarctic krill protein hydrolysate to manage hypertension and diabetes. Article in Journal/Newspaper Antarc* Antarctic Antarctic Krill Wiley Online Library Antarctic The Antarctic Journal of Food Science 82 7 1742 1749 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Inhibition of dipeptidyl peptidase IV (DPP‐IV) and angiotensin converting enzyme (ACE) are considered useful in managing 2 often associated conditions: diabetes and hypertension. In this study, corolase PP was used to hydrolyze Antarctic krill protein. The hydrolysate (AKH) was isolated by ultrafiltration and purified by size‐exclusion chromatography, ion exchange chromatography and reversed‐phase high‐performance liquid chromatography (RP‐HPLC) sequentially. The in vitro inhibitory activities of all AKHs and several fractions obtained against ACE and DPP‐IV were assessed. Two peptides, purified with dual‐strength inhibitory activity against ACE and DPP‐IV, were identified by TOF‐MS/MS. Results indicated that not all fractions exhibited dual inhibitory activities of ACE and DPP‐IV. The purified peptide Lys‐Val‐Glu‐Pro‐Leu‐Pro had half‐maximal inhibitory concentrations (IC 50 ) of 0.93±0.05 and 0.73±0.04 mg/mL against ACE and DPP‐IV, respectively. The other peptide Pro‐Ala‐Leu had IC 50 values of 0.64±0.05 and 0.88±0.03 mg/mL against ACE and DPP‐IV, respectively. This study firstly reported the sequences of dual bioactive peptides from Antarctic krill proteins, further provided new insights into the bioactive peptides responsible for the ACE and DPP‐IV inhibitory activities from the Antarctic krill protein hydrolysate to manage hypertension and diabetes. |
author2 |
Guangdong Ocean University Strong School Innovation Fund Project Disciplinary Construction Project of Institutions of Higher Learning in Guangdong Province Industry-University Research Cooperation Project in Guangdong Province Modern Agro-industry Technology Research System of China |
format |
Article in Journal/Newspaper |
author |
Ji, Wei Zhang, Chaohua Ji, Hongwu |
spellingShingle |
Ji, Wei Zhang, Chaohua Ji, Hongwu Two Novel Bioactive Peptides from Antarctic Krill with Dual Angiotensin Converting Enzyme and Dipeptidyl Peptidase IV Inhibitory Activities |
author_facet |
Ji, Wei Zhang, Chaohua Ji, Hongwu |
author_sort |
Ji, Wei |
title |
Two Novel Bioactive Peptides from Antarctic Krill with Dual Angiotensin Converting Enzyme and Dipeptidyl Peptidase IV Inhibitory Activities |
title_short |
Two Novel Bioactive Peptides from Antarctic Krill with Dual Angiotensin Converting Enzyme and Dipeptidyl Peptidase IV Inhibitory Activities |
title_full |
Two Novel Bioactive Peptides from Antarctic Krill with Dual Angiotensin Converting Enzyme and Dipeptidyl Peptidase IV Inhibitory Activities |
title_fullStr |
Two Novel Bioactive Peptides from Antarctic Krill with Dual Angiotensin Converting Enzyme and Dipeptidyl Peptidase IV Inhibitory Activities |
title_full_unstemmed |
Two Novel Bioactive Peptides from Antarctic Krill with Dual Angiotensin Converting Enzyme and Dipeptidyl Peptidase IV Inhibitory Activities |
title_sort |
two novel bioactive peptides from antarctic krill with dual angiotensin converting enzyme and dipeptidyl peptidase iv inhibitory activities |
publisher |
Wiley |
publishDate |
2017 |
url |
http://dx.doi.org/10.1111/1750-3841.13735 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1750-3841.13735 http://onlinelibrary.wiley.com/wol1/doi/10.1111/1750-3841.13735/fullpdf |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctic Krill |
genre_facet |
Antarc* Antarctic Antarctic Krill |
op_source |
Journal of Food Science volume 82, issue 7, page 1742-1749 ISSN 0022-1147 1750-3841 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/1750-3841.13735 |
container_title |
Journal of Food Science |
container_volume |
82 |
container_issue |
7 |
container_start_page |
1742 |
op_container_end_page |
1749 |
_version_ |
1800740642758852608 |