Molecular identification and phylogenetic analysis of Papaver based on ITS2 barcoding
Abstract In forensic cases suspected to involve Papaver somniferum , species identification is key to the investigation. To accurately detect and identify P. somniferum as well as common adulterants of the same genus, 19 internal transcribed spacer 2 (ITS2) sequences of P . somniferum (256 bp), Papa...
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crwiley:10.1111/1556-4029.14925 2024-10-13T14:10:08+00:00 Molecular identification and phylogenetic analysis of Papaver based on ITS2 barcoding Cheng, Liu Lin, Ziqing Xin, Cairui Sun, Haolin Li, Xinyu 2021 http://dx.doi.org/10.1111/1556-4029.14925 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1556-4029.14925 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1556-4029.14925 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Forensic Sciences volume 67, issue 2, page 712-719 ISSN 0022-1198 1556-4029 journal-article 2021 crwiley https://doi.org/10.1111/1556-4029.14925 2024-09-17T04:50:45Z Abstract In forensic cases suspected to involve Papaver somniferum , species identification is key to the investigation. To accurately detect and identify P. somniferum as well as common adulterants of the same genus, 19 internal transcribed spacer 2 (ITS2) sequences of P . somniferum (256 bp), Papaver canescens (254 bp), Papaver nudicaule (254 bp), Papaver pavoninum (250 bp), Papaver radicatum (254 bp), and Papaver rhoeas (256 bp) were obtained. Based on the ITS2 sequence, similarity analysis via BLAST, the nearest Kimura‐2‐parameter (K2P) genetic distances were calculated, and a phylogenetic tree was constructed using MEGA X software for the identification of six species of Papaver . Finally, differences in the ITS2 secondary structure between species were analyzed. The best matches of the P . somniferum ITS2 sequence were of other P . somniferum from different sources. The nearest K2P genetic distances between P . somniferum and its counterparts from other sources were zero, which was the smallest pairwise genetic distance among distances from the other five Papaver species. Various sources of P . somniferum clustered into an independent branch in the phylogenetic tree. The secondary structures of P . somniferum and P. rhoeas were significantly different from those of the other four species of Papaver. In summary, P . somniferum can be effectively distinguished from five closely related plants of the same genus by using ITS2 as a DNA barcode. Article in Journal/Newspaper Papaver radicatum Wiley Online Library Journal of Forensic Sciences 67 2 712 719 |
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English |
description |
Abstract In forensic cases suspected to involve Papaver somniferum , species identification is key to the investigation. To accurately detect and identify P. somniferum as well as common adulterants of the same genus, 19 internal transcribed spacer 2 (ITS2) sequences of P . somniferum (256 bp), Papaver canescens (254 bp), Papaver nudicaule (254 bp), Papaver pavoninum (250 bp), Papaver radicatum (254 bp), and Papaver rhoeas (256 bp) were obtained. Based on the ITS2 sequence, similarity analysis via BLAST, the nearest Kimura‐2‐parameter (K2P) genetic distances were calculated, and a phylogenetic tree was constructed using MEGA X software for the identification of six species of Papaver . Finally, differences in the ITS2 secondary structure between species were analyzed. The best matches of the P . somniferum ITS2 sequence were of other P . somniferum from different sources. The nearest K2P genetic distances between P . somniferum and its counterparts from other sources were zero, which was the smallest pairwise genetic distance among distances from the other five Papaver species. Various sources of P . somniferum clustered into an independent branch in the phylogenetic tree. The secondary structures of P . somniferum and P. rhoeas were significantly different from those of the other four species of Papaver. In summary, P . somniferum can be effectively distinguished from five closely related plants of the same genus by using ITS2 as a DNA barcode. |
format |
Article in Journal/Newspaper |
author |
Cheng, Liu Lin, Ziqing Xin, Cairui Sun, Haolin Li, Xinyu |
spellingShingle |
Cheng, Liu Lin, Ziqing Xin, Cairui Sun, Haolin Li, Xinyu Molecular identification and phylogenetic analysis of Papaver based on ITS2 barcoding |
author_facet |
Cheng, Liu Lin, Ziqing Xin, Cairui Sun, Haolin Li, Xinyu |
author_sort |
Cheng, Liu |
title |
Molecular identification and phylogenetic analysis of Papaver based on ITS2 barcoding |
title_short |
Molecular identification and phylogenetic analysis of Papaver based on ITS2 barcoding |
title_full |
Molecular identification and phylogenetic analysis of Papaver based on ITS2 barcoding |
title_fullStr |
Molecular identification and phylogenetic analysis of Papaver based on ITS2 barcoding |
title_full_unstemmed |
Molecular identification and phylogenetic analysis of Papaver based on ITS2 barcoding |
title_sort |
molecular identification and phylogenetic analysis of papaver based on its2 barcoding |
publisher |
Wiley |
publishDate |
2021 |
url |
http://dx.doi.org/10.1111/1556-4029.14925 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1556-4029.14925 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1556-4029.14925 |
genre |
Papaver radicatum |
genre_facet |
Papaver radicatum |
op_source |
Journal of Forensic Sciences volume 67, issue 2, page 712-719 ISSN 0022-1198 1556-4029 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/1556-4029.14925 |
container_title |
Journal of Forensic Sciences |
container_volume |
67 |
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2 |
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712 |
op_container_end_page |
719 |
_version_ |
1812817296060579840 |