The exometabolome of microbial communities inhabiting bare ice surfaces on the southern Greenland Ice Sheet
Abstract Microbial blooms colonize the Greenland Ice Sheet bare ice surface during the ablation season and significantly reduce its albedo. On the ice surface, microbes are exposed to high levels of irradiance, freeze–thaw cycles, and low nutrient concentrations. It is well known that microorganisms...
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Online Access: | http://dx.doi.org/10.1111/1462-2920.16574 |
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crwiley:10.1111/1462-2920.16574 2024-09-09T19:41:24+00:00 The exometabolome of microbial communities inhabiting bare ice surfaces on the southern Greenland Ice Sheet Doting, Eva L. Jensen, Marie B. Peter, Elisa K. Ellegaard‐Jensen, Lea Tranter, Martyn Benning, Liane G. Hansen, Martin Anesio, Alexandre M. Aarhus Universitets Forskningsfond H2020 European Research Council 2024 http://dx.doi.org/10.1111/1462-2920.16574 en eng Wiley http://creativecommons.org/licenses/by/4.0/ Environmental Microbiology volume 26, issue 2 ISSN 1462-2912 1462-2920 journal-article 2024 crwiley https://doi.org/10.1111/1462-2920.16574 2024-08-13T04:16:46Z Abstract Microbial blooms colonize the Greenland Ice Sheet bare ice surface during the ablation season and significantly reduce its albedo. On the ice surface, microbes are exposed to high levels of irradiance, freeze–thaw cycles, and low nutrient concentrations. It is well known that microorganisms secrete metabolites to maintain homeostasis, communicate with other microorganisms, and defend themselves. Yet, the exometabolome of supraglacial microbial blooms, dominated by the pigmented glacier ice algae Ancylonema alaskanum and Ancylonema nordenskiöldii , remains thus far unstudied. Here, we use a high‐resolution mass spectrometry‐based untargeted metabolomics workflow to identify metabolites in the exometabolome of microbial blooms on the surface of the southern tip of the Greenland Ice Sheet. Samples were collected every 6 h across two diurnal cycles at 5 replicate sampling sites with high similarity in community composition, in terms of orders and phyla present. Time of sampling explained 46% (permutational multivariate analysis of variance [PERMANOVA], pseudo‐ F = 3.7771, p = 0.001) and 27% (PERMANOVA, pseudo‐ F = 1.8705, p = 0.001) of variance in the exometabolome across the two diurnal cycles. Annotated metabolites included riboflavin, lumichrome, tryptophan, and azelaic acid, all of which have demonstrated roles in microbe–microbe interactions in other ecosystems and should be tested for potential roles in the development of microbial blooms on bare ice surfaces. Article in Journal/Newspaper glacier Greenland ice algae Ice Sheet Wiley Online Library Greenland Environmental Microbiology 26 2 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Microbial blooms colonize the Greenland Ice Sheet bare ice surface during the ablation season and significantly reduce its albedo. On the ice surface, microbes are exposed to high levels of irradiance, freeze–thaw cycles, and low nutrient concentrations. It is well known that microorganisms secrete metabolites to maintain homeostasis, communicate with other microorganisms, and defend themselves. Yet, the exometabolome of supraglacial microbial blooms, dominated by the pigmented glacier ice algae Ancylonema alaskanum and Ancylonema nordenskiöldii , remains thus far unstudied. Here, we use a high‐resolution mass spectrometry‐based untargeted metabolomics workflow to identify metabolites in the exometabolome of microbial blooms on the surface of the southern tip of the Greenland Ice Sheet. Samples were collected every 6 h across two diurnal cycles at 5 replicate sampling sites with high similarity in community composition, in terms of orders and phyla present. Time of sampling explained 46% (permutational multivariate analysis of variance [PERMANOVA], pseudo‐ F = 3.7771, p = 0.001) and 27% (PERMANOVA, pseudo‐ F = 1.8705, p = 0.001) of variance in the exometabolome across the two diurnal cycles. Annotated metabolites included riboflavin, lumichrome, tryptophan, and azelaic acid, all of which have demonstrated roles in microbe–microbe interactions in other ecosystems and should be tested for potential roles in the development of microbial blooms on bare ice surfaces. |
author2 |
Aarhus Universitets Forskningsfond H2020 European Research Council |
format |
Article in Journal/Newspaper |
author |
Doting, Eva L. Jensen, Marie B. Peter, Elisa K. Ellegaard‐Jensen, Lea Tranter, Martyn Benning, Liane G. Hansen, Martin Anesio, Alexandre M. |
spellingShingle |
Doting, Eva L. Jensen, Marie B. Peter, Elisa K. Ellegaard‐Jensen, Lea Tranter, Martyn Benning, Liane G. Hansen, Martin Anesio, Alexandre M. The exometabolome of microbial communities inhabiting bare ice surfaces on the southern Greenland Ice Sheet |
author_facet |
Doting, Eva L. Jensen, Marie B. Peter, Elisa K. Ellegaard‐Jensen, Lea Tranter, Martyn Benning, Liane G. Hansen, Martin Anesio, Alexandre M. |
author_sort |
Doting, Eva L. |
title |
The exometabolome of microbial communities inhabiting bare ice surfaces on the southern Greenland Ice Sheet |
title_short |
The exometabolome of microbial communities inhabiting bare ice surfaces on the southern Greenland Ice Sheet |
title_full |
The exometabolome of microbial communities inhabiting bare ice surfaces on the southern Greenland Ice Sheet |
title_fullStr |
The exometabolome of microbial communities inhabiting bare ice surfaces on the southern Greenland Ice Sheet |
title_full_unstemmed |
The exometabolome of microbial communities inhabiting bare ice surfaces on the southern Greenland Ice Sheet |
title_sort |
exometabolome of microbial communities inhabiting bare ice surfaces on the southern greenland ice sheet |
publisher |
Wiley |
publishDate |
2024 |
url |
http://dx.doi.org/10.1111/1462-2920.16574 |
geographic |
Greenland |
geographic_facet |
Greenland |
genre |
glacier Greenland ice algae Ice Sheet |
genre_facet |
glacier Greenland ice algae Ice Sheet |
op_source |
Environmental Microbiology volume 26, issue 2 ISSN 1462-2912 1462-2920 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1111/1462-2920.16574 |
container_title |
Environmental Microbiology |
container_volume |
26 |
container_issue |
2 |
_version_ |
1809910801824219136 |