Altering environmental conditions induce shifts in simulated deep terrestrial subsurface bacterial communities—Secretion of primary and secondary metabolites
Abstract The deep terrestrial subsurface (DTS) harbours a striking diversity of microorganisms. However, systematic research on microbial metabolism, and how varying groundwater composition affects the bacterial communities and metabolites in these environments is lacking. In this study, DTS groundw...
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crwiley:10.1111/1462-2920.16552 2024-06-02T08:06:32+00:00 Altering environmental conditions induce shifts in simulated deep terrestrial subsurface bacterial communities—Secretion of primary and secondary metabolites Herzig, Merja Hyötyläinen, Tuulia Vettese, Gianni F. Law, Gareth T. W. Vierinen, Taavi Bomberg, Malin 2023 http://dx.doi.org/10.1111/1462-2920.16552 en eng Wiley http://creativecommons.org/licenses/by-nc-nd/4.0/ Environmental Microbiology volume 26, issue 1 ISSN 1462-2912 1462-2920 journal-article 2023 crwiley https://doi.org/10.1111/1462-2920.16552 2024-05-03T11:00:48Z Abstract The deep terrestrial subsurface (DTS) harbours a striking diversity of microorganisms. However, systematic research on microbial metabolism, and how varying groundwater composition affects the bacterial communities and metabolites in these environments is lacking. In this study, DTS groundwater bacterial consortia from two Fennoscandian Shield sites were enriched and studied. We found that the enriched communities from the two sites consisted of distinct bacterial taxa, and alterations in the growth medium composition induced changes in cell counts. The lack of an exogenous organic carbon source (ECS) caused a notable increase in lipid metabolism in one community, while in the other, carbon starvation resulted in low overall metabolism, suggesting a dormant state. ECS supplementation increased CO 2 production and SO 4 2− utilisation, suggesting activation of a dissimilatory sulphate reduction pathway and sulphate‐reducer‐dominated total metabolism. However, both communities shared common universal metabolic features, most probably involving pathways needed for the maintenance of cell homeostasis (e.g., mevalonic acid pathway). Collectively, our findings indicate that the most important metabolites related to microbial reactions under varying growth conditions in enriched DTS communities include, but are not limited to, those linked to cell homeostasis, osmoregulation, lipid biosynthesis and degradation, dissimilatory sulphate reduction and isoprenoid production. Article in Journal/Newspaper Fennoscandian Wiley Online Library Environmental Microbiology 26 1 |
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description |
Abstract The deep terrestrial subsurface (DTS) harbours a striking diversity of microorganisms. However, systematic research on microbial metabolism, and how varying groundwater composition affects the bacterial communities and metabolites in these environments is lacking. In this study, DTS groundwater bacterial consortia from two Fennoscandian Shield sites were enriched and studied. We found that the enriched communities from the two sites consisted of distinct bacterial taxa, and alterations in the growth medium composition induced changes in cell counts. The lack of an exogenous organic carbon source (ECS) caused a notable increase in lipid metabolism in one community, while in the other, carbon starvation resulted in low overall metabolism, suggesting a dormant state. ECS supplementation increased CO 2 production and SO 4 2− utilisation, suggesting activation of a dissimilatory sulphate reduction pathway and sulphate‐reducer‐dominated total metabolism. However, both communities shared common universal metabolic features, most probably involving pathways needed for the maintenance of cell homeostasis (e.g., mevalonic acid pathway). Collectively, our findings indicate that the most important metabolites related to microbial reactions under varying growth conditions in enriched DTS communities include, but are not limited to, those linked to cell homeostasis, osmoregulation, lipid biosynthesis and degradation, dissimilatory sulphate reduction and isoprenoid production. |
format |
Article in Journal/Newspaper |
author |
Herzig, Merja Hyötyläinen, Tuulia Vettese, Gianni F. Law, Gareth T. W. Vierinen, Taavi Bomberg, Malin |
spellingShingle |
Herzig, Merja Hyötyläinen, Tuulia Vettese, Gianni F. Law, Gareth T. W. Vierinen, Taavi Bomberg, Malin Altering environmental conditions induce shifts in simulated deep terrestrial subsurface bacterial communities—Secretion of primary and secondary metabolites |
author_facet |
Herzig, Merja Hyötyläinen, Tuulia Vettese, Gianni F. Law, Gareth T. W. Vierinen, Taavi Bomberg, Malin |
author_sort |
Herzig, Merja |
title |
Altering environmental conditions induce shifts in simulated deep terrestrial subsurface bacterial communities—Secretion of primary and secondary metabolites |
title_short |
Altering environmental conditions induce shifts in simulated deep terrestrial subsurface bacterial communities—Secretion of primary and secondary metabolites |
title_full |
Altering environmental conditions induce shifts in simulated deep terrestrial subsurface bacterial communities—Secretion of primary and secondary metabolites |
title_fullStr |
Altering environmental conditions induce shifts in simulated deep terrestrial subsurface bacterial communities—Secretion of primary and secondary metabolites |
title_full_unstemmed |
Altering environmental conditions induce shifts in simulated deep terrestrial subsurface bacterial communities—Secretion of primary and secondary metabolites |
title_sort |
altering environmental conditions induce shifts in simulated deep terrestrial subsurface bacterial communities—secretion of primary and secondary metabolites |
publisher |
Wiley |
publishDate |
2023 |
url |
http://dx.doi.org/10.1111/1462-2920.16552 |
genre |
Fennoscandian |
genre_facet |
Fennoscandian |
op_source |
Environmental Microbiology volume 26, issue 1 ISSN 1462-2912 1462-2920 |
op_rights |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
op_doi |
https://doi.org/10.1111/1462-2920.16552 |
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Environmental Microbiology |
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26 |
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1 |
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1800751488650182656 |