Environmental predictors of electroactive bacterioplankton in small boreal lakes
Abstract Extracellular electron transfer (EET) by electroactive bacteria in anoxic soils and sediments is an intensively researched subject, but EET's function in planktonic ecology has been less considered. Following the discovery of an unexpectedly high prevalence of EET genes in a bog lake...
Published in: | Environmental Microbiology |
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Online Access: | http://dx.doi.org/10.1111/1462-2920.16314 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.16314 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.16314 https://sfamjournals.onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.16314 |
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crwiley:10.1111/1462-2920.16314 2024-10-13T14:10:15+00:00 Environmental predictors of electroactive bacterioplankton in small boreal lakes Olmsted, Charles N. Ort, Roger Tran, Patricia Q. McDaniel, Elizabeth A. Roden, Eric E. Bond, Daniel R. He, Shaomei McMahon, Katherine D. National Science Foundation 2022 http://dx.doi.org/10.1111/1462-2920.16314 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.16314 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.16314 https://sfamjournals.onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.16314 en eng Wiley http://creativecommons.org/licenses/by-nc/4.0/ http://creativecommons.org/licenses/by-nc/4.0/ Environmental Microbiology volume 25, issue 3, page 705-720 ISSN 1462-2912 1462-2920 journal-article 2022 crwiley https://doi.org/10.1111/1462-2920.16314 2024-09-17T04:51:33Z Abstract Extracellular electron transfer (EET) by electroactive bacteria in anoxic soils and sediments is an intensively researched subject, but EET's function in planktonic ecology has been less considered. Following the discovery of an unexpectedly high prevalence of EET genes in a bog lake's bacterioplankton, we hypothesized that the redox capacities of dissolved organic matter (DOM) enrich for electroactive bacteria by mediating redox chemistry. We developed the bioinformatics pipeline FEET (Find EET) to identify and summarize predicted EET protein‐encoding genes from metagenomics data. We then applied FEET to 36 bog and thermokarst lakes and correlated gene occurrence with environmental data to test our predictions. Our results provide indirect evidence that DOM may participate in bacterioplankton EET. We found a similarly high prevalence of genes encoding putative EET proteins in most of these lakes, where oxidative EET strongly correlated with DOM. Numerous novel clusters of multiheme cytochromes that may enable EET were identified. Taxa previously not considered EET‐capable were found to carry EET genes. We propose that EET and DOM interactions are of ecologically important to bacterioplankton in small boreal lakes, and that EET, particularly by methylotrophs and anoxygenic phototrophs, should be further studied and incorporated into methane emission models of melting permafrost. Article in Journal/Newspaper permafrost Thermokarst Wiley Online Library Environmental Microbiology 25 3 705 720 |
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Wiley Online Library |
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crwiley |
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English |
description |
Abstract Extracellular electron transfer (EET) by electroactive bacteria in anoxic soils and sediments is an intensively researched subject, but EET's function in planktonic ecology has been less considered. Following the discovery of an unexpectedly high prevalence of EET genes in a bog lake's bacterioplankton, we hypothesized that the redox capacities of dissolved organic matter (DOM) enrich for electroactive bacteria by mediating redox chemistry. We developed the bioinformatics pipeline FEET (Find EET) to identify and summarize predicted EET protein‐encoding genes from metagenomics data. We then applied FEET to 36 bog and thermokarst lakes and correlated gene occurrence with environmental data to test our predictions. Our results provide indirect evidence that DOM may participate in bacterioplankton EET. We found a similarly high prevalence of genes encoding putative EET proteins in most of these lakes, where oxidative EET strongly correlated with DOM. Numerous novel clusters of multiheme cytochromes that may enable EET were identified. Taxa previously not considered EET‐capable were found to carry EET genes. We propose that EET and DOM interactions are of ecologically important to bacterioplankton in small boreal lakes, and that EET, particularly by methylotrophs and anoxygenic phototrophs, should be further studied and incorporated into methane emission models of melting permafrost. |
author2 |
National Science Foundation |
format |
Article in Journal/Newspaper |
author |
Olmsted, Charles N. Ort, Roger Tran, Patricia Q. McDaniel, Elizabeth A. Roden, Eric E. Bond, Daniel R. He, Shaomei McMahon, Katherine D. |
spellingShingle |
Olmsted, Charles N. Ort, Roger Tran, Patricia Q. McDaniel, Elizabeth A. Roden, Eric E. Bond, Daniel R. He, Shaomei McMahon, Katherine D. Environmental predictors of electroactive bacterioplankton in small boreal lakes |
author_facet |
Olmsted, Charles N. Ort, Roger Tran, Patricia Q. McDaniel, Elizabeth A. Roden, Eric E. Bond, Daniel R. He, Shaomei McMahon, Katherine D. |
author_sort |
Olmsted, Charles N. |
title |
Environmental predictors of electroactive bacterioplankton in small boreal lakes |
title_short |
Environmental predictors of electroactive bacterioplankton in small boreal lakes |
title_full |
Environmental predictors of electroactive bacterioplankton in small boreal lakes |
title_fullStr |
Environmental predictors of electroactive bacterioplankton in small boreal lakes |
title_full_unstemmed |
Environmental predictors of electroactive bacterioplankton in small boreal lakes |
title_sort |
environmental predictors of electroactive bacterioplankton in small boreal lakes |
publisher |
Wiley |
publishDate |
2022 |
url |
http://dx.doi.org/10.1111/1462-2920.16314 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.16314 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.16314 https://sfamjournals.onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.16314 |
genre |
permafrost Thermokarst |
genre_facet |
permafrost Thermokarst |
op_source |
Environmental Microbiology volume 25, issue 3, page 705-720 ISSN 1462-2912 1462-2920 |
op_rights |
http://creativecommons.org/licenses/by-nc/4.0/ http://creativecommons.org/licenses/by-nc/4.0/ |
op_doi |
https://doi.org/10.1111/1462-2920.16314 |
container_title |
Environmental Microbiology |
container_volume |
25 |
container_issue |
3 |
container_start_page |
705 |
op_container_end_page |
720 |
_version_ |
1812817434941325312 |