Microbial metabolism and adaptations in Atribacteria‐dominated methane hydrate sediments
Summary Gas hydrates harbour gigatons of natural gas, yet their microbiomes remain understudied. We bioprospected 16S rRNA amplicons, metagenomes, and metaproteomes from methane hydrate‐bearing sediments under Hydrate Ridge (offshore Oregon, USA, ODP Site 1244, 2–69 mbsf) for novel microbial metabol...
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crwiley:10.1111/1462-2920.15656 2024-06-23T07:54:37+00:00 Microbial metabolism and adaptations in Atribacteria‐dominated methane hydrate sediments Glass, Jennifer B. Ranjan, Piyush Kretz, Cecilia B. Nunn, Brook L. Johnson, Abigail M. Xu, Manlin McManus, James Stewart, Frank J. Division of Ocean Sciences Center for Dark Energy Biosphere Investigations Center for Dark Energy Biosphere Investigations 2021 http://dx.doi.org/10.1111/1462-2920.15656 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.15656 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.15656 https://sfamjournals.onlinelibrary.wiley.com/doi/am-pdf/10.1111/1462-2920.15656 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#am http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Microbiology volume 23, issue 8, page 4646-4660 ISSN 1462-2912 1462-2920 journal-article 2021 crwiley https://doi.org/10.1111/1462-2920.15656 2024-06-11T04:50:10Z Summary Gas hydrates harbour gigatons of natural gas, yet their microbiomes remain understudied. We bioprospected 16S rRNA amplicons, metagenomes, and metaproteomes from methane hydrate‐bearing sediments under Hydrate Ridge (offshore Oregon, USA, ODP Site 1244, 2–69 mbsf) for novel microbial metabolic and biosynthetic potential. Atribacteria sequences generally increased in relative sequence abundance with increasing sediment depth. Most Atribacteria ASVs belonged to JS‐1‐Genus 1 and clustered with other sequences from gas hydrate‐bearing sediments. We recovered 21 metagenome‐assembled genomic bins spanning three geochemical zones in the sediment core: the sulfate–methane transition zone, the metal (iron/manganese) reduction zone, and the gas hydrate stability zone. We found evidence for bacterial fermentation as a source of acetate for aceticlastic methanogenesis and as a driver of iron reduction in the metal reduction zone. In multiple zones, we identified a Ni‐Fe hydrogenase‐Na + /H + antiporter supercomplex (Hun) in Atribacteria and Firmicutes bins and in other deep subsurface bacteria and cultured hyperthermophiles from the Thermotogae phylum. Atribacteria expressed tripartite ATP‐independent transporters downstream from a novel regulator (AtiR). Atribacteria also possessed adaptations to survive extreme conditions (e.g. high salt brines, high pressure and cold temperatures) including the ability to synthesize the osmolyte di‐myo‐inositol‐phosphate as well as expression of K + ‐stimulated pyrophosphatase and capsule proteins. Article in Journal/Newspaper Methane hydrate Wiley Online Library Environmental Microbiology 23 8 4646 4660 |
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Wiley Online Library |
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crwiley |
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English |
description |
Summary Gas hydrates harbour gigatons of natural gas, yet their microbiomes remain understudied. We bioprospected 16S rRNA amplicons, metagenomes, and metaproteomes from methane hydrate‐bearing sediments under Hydrate Ridge (offshore Oregon, USA, ODP Site 1244, 2–69 mbsf) for novel microbial metabolic and biosynthetic potential. Atribacteria sequences generally increased in relative sequence abundance with increasing sediment depth. Most Atribacteria ASVs belonged to JS‐1‐Genus 1 and clustered with other sequences from gas hydrate‐bearing sediments. We recovered 21 metagenome‐assembled genomic bins spanning three geochemical zones in the sediment core: the sulfate–methane transition zone, the metal (iron/manganese) reduction zone, and the gas hydrate stability zone. We found evidence for bacterial fermentation as a source of acetate for aceticlastic methanogenesis and as a driver of iron reduction in the metal reduction zone. In multiple zones, we identified a Ni‐Fe hydrogenase‐Na + /H + antiporter supercomplex (Hun) in Atribacteria and Firmicutes bins and in other deep subsurface bacteria and cultured hyperthermophiles from the Thermotogae phylum. Atribacteria expressed tripartite ATP‐independent transporters downstream from a novel regulator (AtiR). Atribacteria also possessed adaptations to survive extreme conditions (e.g. high salt brines, high pressure and cold temperatures) including the ability to synthesize the osmolyte di‐myo‐inositol‐phosphate as well as expression of K + ‐stimulated pyrophosphatase and capsule proteins. |
author2 |
Division of Ocean Sciences Center for Dark Energy Biosphere Investigations Center for Dark Energy Biosphere Investigations |
format |
Article in Journal/Newspaper |
author |
Glass, Jennifer B. Ranjan, Piyush Kretz, Cecilia B. Nunn, Brook L. Johnson, Abigail M. Xu, Manlin McManus, James Stewart, Frank J. |
spellingShingle |
Glass, Jennifer B. Ranjan, Piyush Kretz, Cecilia B. Nunn, Brook L. Johnson, Abigail M. Xu, Manlin McManus, James Stewart, Frank J. Microbial metabolism and adaptations in Atribacteria‐dominated methane hydrate sediments |
author_facet |
Glass, Jennifer B. Ranjan, Piyush Kretz, Cecilia B. Nunn, Brook L. Johnson, Abigail M. Xu, Manlin McManus, James Stewart, Frank J. |
author_sort |
Glass, Jennifer B. |
title |
Microbial metabolism and adaptations in Atribacteria‐dominated methane hydrate sediments |
title_short |
Microbial metabolism and adaptations in Atribacteria‐dominated methane hydrate sediments |
title_full |
Microbial metabolism and adaptations in Atribacteria‐dominated methane hydrate sediments |
title_fullStr |
Microbial metabolism and adaptations in Atribacteria‐dominated methane hydrate sediments |
title_full_unstemmed |
Microbial metabolism and adaptations in Atribacteria‐dominated methane hydrate sediments |
title_sort |
microbial metabolism and adaptations in atribacteria‐dominated methane hydrate sediments |
publisher |
Wiley |
publishDate |
2021 |
url |
http://dx.doi.org/10.1111/1462-2920.15656 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.15656 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.15656 https://sfamjournals.onlinelibrary.wiley.com/doi/am-pdf/10.1111/1462-2920.15656 |
genre |
Methane hydrate |
genre_facet |
Methane hydrate |
op_source |
Environmental Microbiology volume 23, issue 8, page 4646-4660 ISSN 1462-2912 1462-2920 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#am http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/1462-2920.15656 |
container_title |
Environmental Microbiology |
container_volume |
23 |
container_issue |
8 |
container_start_page |
4646 |
op_container_end_page |
4660 |
_version_ |
1802646820444176384 |