Genomic diversity and CRISPR‐Cas systems in the cyanobacterium Nostoc in the High Arctic
Summary Nostoc (Nostocales, Cyanobacteria) has a global distribution in the Polar Regions. However, the genomic diversity of Nostoc is little known and there are no genomes available for polar Nostoc . Here we carried out the first genomic analysis of the Nostoc commune morphotype with a recent samp...
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crwiley:10.1111/1462-2920.15481 2024-06-02T08:00:33+00:00 Genomic diversity and CRISPR‐Cas systems in the cyanobacterium Nostoc in the High Arctic Jungblut, Anne D. Raymond, Frédéric Dion, Moïra B. Moineau, Sylvain Mohit, Vani Nguyen, Guillaume Quang Déraspe, Maxime Francovic‐Fontaine, Élina Lovejoy, Connie Culley, Alexander I. Corbeil, Jacques Vincent, Warwick F. ArcticNet Natural Sciences and Engineering Research Council of Canada 2021 http://dx.doi.org/10.1111/1462-2920.15481 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.15481 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.15481 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Microbiology volume 23, issue 6, page 2955-2968 ISSN 1462-2912 1462-2920 journal-article 2021 crwiley https://doi.org/10.1111/1462-2920.15481 2024-05-03T10:54:01Z Summary Nostoc (Nostocales, Cyanobacteria) has a global distribution in the Polar Regions. However, the genomic diversity of Nostoc is little known and there are no genomes available for polar Nostoc . Here we carried out the first genomic analysis of the Nostoc commune morphotype with a recent sample from the High Arctic and a herbarium specimen collected during the British Arctic Expedition (1875–76). Comparisons of the polar genomes with 26 present‐day non‐polar members of the Nostocales family highlighted that there are pronounced genetic variations among Nostoc strains and species. Osmoprotection and other stress genes were found in all Nostoc strains, but the two Arctic strains had markedly higher numbers of biosynthetic gene clusters for uncharacterised non‐ribosomal peptide synthetases, suggesting a high diversity of secondary metabolites. Since viral–host interactions contribute to microbial diversity, we analysed the CRISPR‐Cas systems in the Arctic and two temperate Nostoc species. There were a large number of unique repeat‐spacer arrays in each genome, indicating diverse histories of viral attack. All Nostoc strains had a subtype I‐D system, but the polar specimens also showed evidence of a subtype I‐B system that has not been previously reported in cyanobacteria, suggesting diverse cyanobacteria–virus interactions in the Arctic. Article in Journal/Newspaper Arctic Wiley Online Library Arctic Environmental Microbiology |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Summary Nostoc (Nostocales, Cyanobacteria) has a global distribution in the Polar Regions. However, the genomic diversity of Nostoc is little known and there are no genomes available for polar Nostoc . Here we carried out the first genomic analysis of the Nostoc commune morphotype with a recent sample from the High Arctic and a herbarium specimen collected during the British Arctic Expedition (1875–76). Comparisons of the polar genomes with 26 present‐day non‐polar members of the Nostocales family highlighted that there are pronounced genetic variations among Nostoc strains and species. Osmoprotection and other stress genes were found in all Nostoc strains, but the two Arctic strains had markedly higher numbers of biosynthetic gene clusters for uncharacterised non‐ribosomal peptide synthetases, suggesting a high diversity of secondary metabolites. Since viral–host interactions contribute to microbial diversity, we analysed the CRISPR‐Cas systems in the Arctic and two temperate Nostoc species. There were a large number of unique repeat‐spacer arrays in each genome, indicating diverse histories of viral attack. All Nostoc strains had a subtype I‐D system, but the polar specimens also showed evidence of a subtype I‐B system that has not been previously reported in cyanobacteria, suggesting diverse cyanobacteria–virus interactions in the Arctic. |
author2 |
ArcticNet Natural Sciences and Engineering Research Council of Canada |
format |
Article in Journal/Newspaper |
author |
Jungblut, Anne D. Raymond, Frédéric Dion, Moïra B. Moineau, Sylvain Mohit, Vani Nguyen, Guillaume Quang Déraspe, Maxime Francovic‐Fontaine, Élina Lovejoy, Connie Culley, Alexander I. Corbeil, Jacques Vincent, Warwick F. |
spellingShingle |
Jungblut, Anne D. Raymond, Frédéric Dion, Moïra B. Moineau, Sylvain Mohit, Vani Nguyen, Guillaume Quang Déraspe, Maxime Francovic‐Fontaine, Élina Lovejoy, Connie Culley, Alexander I. Corbeil, Jacques Vincent, Warwick F. Genomic diversity and CRISPR‐Cas systems in the cyanobacterium Nostoc in the High Arctic |
author_facet |
Jungblut, Anne D. Raymond, Frédéric Dion, Moïra B. Moineau, Sylvain Mohit, Vani Nguyen, Guillaume Quang Déraspe, Maxime Francovic‐Fontaine, Élina Lovejoy, Connie Culley, Alexander I. Corbeil, Jacques Vincent, Warwick F. |
author_sort |
Jungblut, Anne D. |
title |
Genomic diversity and CRISPR‐Cas systems in the cyanobacterium Nostoc in the High Arctic |
title_short |
Genomic diversity and CRISPR‐Cas systems in the cyanobacterium Nostoc in the High Arctic |
title_full |
Genomic diversity and CRISPR‐Cas systems in the cyanobacterium Nostoc in the High Arctic |
title_fullStr |
Genomic diversity and CRISPR‐Cas systems in the cyanobacterium Nostoc in the High Arctic |
title_full_unstemmed |
Genomic diversity and CRISPR‐Cas systems in the cyanobacterium Nostoc in the High Arctic |
title_sort |
genomic diversity and crispr‐cas systems in the cyanobacterium nostoc in the high arctic |
publisher |
Wiley |
publishDate |
2021 |
url |
http://dx.doi.org/10.1111/1462-2920.15481 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.15481 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.15481 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Environmental Microbiology volume 23, issue 6, page 2955-2968 ISSN 1462-2912 1462-2920 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/1462-2920.15481 |
container_title |
Environmental Microbiology |
_version_ |
1800744588073238528 |