Revealing environmentally driven population dynamics of an Arctic diatom using a novel microsatellite PoolSeq barcoding approach
Summary Ecological stability under environmental change is determined by both interspecific and intraspecific processes. Particularly for planktonic microorganisms, it is challenging to follow intraspecific dynamics over space and time. We propose a new method, microsatellite PoolSeq barcoding (MPB)...
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Online Access: | http://dx.doi.org/10.1111/1462-2920.15424 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.15424 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.15424 |
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crwiley:10.1111/1462-2920.15424 2024-06-02T08:01:43+00:00 Revealing environmentally driven population dynamics of an Arctic diatom using a novel microsatellite PoolSeq barcoding approach Wolf, Klara K. E. Hoppe, Clara J. M. Leese, Florian Weiss, Martina Rost, Björn Neuhaus, Stefan Gross, Thilo Kühne, Nancy John, Uwe 2021 http://dx.doi.org/10.1111/1462-2920.15424 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.15424 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.15424 en eng Wiley http://creativecommons.org/licenses/by/4.0/ Environmental Microbiology volume 23, issue 7, page 3809-3824 ISSN 1462-2912 1462-2920 journal-article 2021 crwiley https://doi.org/10.1111/1462-2920.15424 2024-05-03T11:20:40Z Summary Ecological stability under environmental change is determined by both interspecific and intraspecific processes. Particularly for planktonic microorganisms, it is challenging to follow intraspecific dynamics over space and time. We propose a new method, microsatellite PoolSeq barcoding (MPB), for tracing allele frequency changes in protist populations. We successfully applied this method to experimental community incubations and field samples of the diatom Thalassiosira hyalina from the Arctic, a rapidly changing ecosystem. Validation of the method found compelling accuracy in comparison with established genotyping approaches within different diversity contexts. In experimental and environmental samples, we show that MPB can detect meaningful patterns of population dynamics, resolving allelic stability and shifts within a key diatom species in response to experimental treatments as well as different bloom phases and years. Through our novel MPB approach, we produced a large dataset of populations at different time‐points and locations with comparably little effort. Results like this can add insights into the roles of selection and plasticity in natural protist populations under stable experimental but also variable field conditions. Especially for organisms where genotype sampling remains challenging, MPB holds great potential to efficiently resolve eco‐evolutionary dynamics and to assess the mechanisms and limits of resilience to environmental stressors. Article in Journal/Newspaper Arctic Wiley Online Library Arctic Environmental Microbiology |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Summary Ecological stability under environmental change is determined by both interspecific and intraspecific processes. Particularly for planktonic microorganisms, it is challenging to follow intraspecific dynamics over space and time. We propose a new method, microsatellite PoolSeq barcoding (MPB), for tracing allele frequency changes in protist populations. We successfully applied this method to experimental community incubations and field samples of the diatom Thalassiosira hyalina from the Arctic, a rapidly changing ecosystem. Validation of the method found compelling accuracy in comparison with established genotyping approaches within different diversity contexts. In experimental and environmental samples, we show that MPB can detect meaningful patterns of population dynamics, resolving allelic stability and shifts within a key diatom species in response to experimental treatments as well as different bloom phases and years. Through our novel MPB approach, we produced a large dataset of populations at different time‐points and locations with comparably little effort. Results like this can add insights into the roles of selection and plasticity in natural protist populations under stable experimental but also variable field conditions. Especially for organisms where genotype sampling remains challenging, MPB holds great potential to efficiently resolve eco‐evolutionary dynamics and to assess the mechanisms and limits of resilience to environmental stressors. |
format |
Article in Journal/Newspaper |
author |
Wolf, Klara K. E. Hoppe, Clara J. M. Leese, Florian Weiss, Martina Rost, Björn Neuhaus, Stefan Gross, Thilo Kühne, Nancy John, Uwe |
spellingShingle |
Wolf, Klara K. E. Hoppe, Clara J. M. Leese, Florian Weiss, Martina Rost, Björn Neuhaus, Stefan Gross, Thilo Kühne, Nancy John, Uwe Revealing environmentally driven population dynamics of an Arctic diatom using a novel microsatellite PoolSeq barcoding approach |
author_facet |
Wolf, Klara K. E. Hoppe, Clara J. M. Leese, Florian Weiss, Martina Rost, Björn Neuhaus, Stefan Gross, Thilo Kühne, Nancy John, Uwe |
author_sort |
Wolf, Klara K. E. |
title |
Revealing environmentally driven population dynamics of an Arctic diatom using a novel microsatellite PoolSeq barcoding approach |
title_short |
Revealing environmentally driven population dynamics of an Arctic diatom using a novel microsatellite PoolSeq barcoding approach |
title_full |
Revealing environmentally driven population dynamics of an Arctic diatom using a novel microsatellite PoolSeq barcoding approach |
title_fullStr |
Revealing environmentally driven population dynamics of an Arctic diatom using a novel microsatellite PoolSeq barcoding approach |
title_full_unstemmed |
Revealing environmentally driven population dynamics of an Arctic diatom using a novel microsatellite PoolSeq barcoding approach |
title_sort |
revealing environmentally driven population dynamics of an arctic diatom using a novel microsatellite poolseq barcoding approach |
publisher |
Wiley |
publishDate |
2021 |
url |
http://dx.doi.org/10.1111/1462-2920.15424 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.15424 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.15424 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Environmental Microbiology volume 23, issue 7, page 3809-3824 ISSN 1462-2912 1462-2920 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1111/1462-2920.15424 |
container_title |
Environmental Microbiology |
_version_ |
1800746115978493952 |