Transcriptomic reprogramming of the oceanic diatom Skeletonema dohrnii under warming ocean and acidification
Summary Under ocean warming and acidification, diatoms use a unique acclimation and adaptation strategy by saving energy and utilizing it for other cellular processes. However, the molecular mechanisms that underlie this reprogramming of energy utilization are currently unknown. Here, we investigate...
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crwiley:10.1111/1462-2920.15248 2024-09-30T14:40:48+00:00 Transcriptomic reprogramming of the oceanic diatom Skeletonema dohrnii under warming ocean and acidification Thangaraj, Satheeswaran Sun, Jun Changjiang Scholar Program of Chinese Ministry of Education National Natural Science Foundation of China 2020 http://dx.doi.org/10.1111/1462-2920.15248 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.15248 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.15248 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Microbiology volume 23, issue 2, page 980-995 ISSN 1462-2912 1462-2920 journal-article 2020 crwiley https://doi.org/10.1111/1462-2920.15248 2024-09-11T04:17:53Z Summary Under ocean warming and acidification, diatoms use a unique acclimation and adaptation strategy by saving energy and utilizing it for other cellular processes. However, the molecular mechanisms that underlie this reprogramming of energy utilization are currently unknown. Here, we investigate the metabolic reprogramming of the ecologically important diatom Skeletonema dohrnii grown under two different temperature (21°C and 25°C) and p CO 2 (400 and 1000 ppm) levels, utilizing global transcriptomic analysis. We find that evolutionary changes in the baseline gene expression, which we termed transcriptional up‐ and downregulation, is the primary mechanism used by diatoms to acclimate to the combined conditions of ocean warming and acidification. This transcriptional regulation shows that under higher temperature and p CO 2 conditions, photosynthesis, electron transport and carboxylation were modified with increasing abundances of genes encoding ATP, NADPH and carbon gaining for the carbon‐dioxide‐concentrating mechanisms (CCMs). Our results also indicate that changes in the transcriptional regulation of CCMs led to a decrease in the metabolic cost to save energy by promoting amino acid synthesis and nitrogen assimilation for the active protein processing machinery to adapt to warming and ocean acidification. This study generated unique metabolic insights into diatoms and suggests that future climate change conditions will cause evolutionary changes in oceanic diatoms that will facilitate their acclimation strategy. Article in Journal/Newspaper Ocean acidification Wiley Online Library Environmental Microbiology 23 2 980 995 |
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Wiley Online Library |
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English |
description |
Summary Under ocean warming and acidification, diatoms use a unique acclimation and adaptation strategy by saving energy and utilizing it for other cellular processes. However, the molecular mechanisms that underlie this reprogramming of energy utilization are currently unknown. Here, we investigate the metabolic reprogramming of the ecologically important diatom Skeletonema dohrnii grown under two different temperature (21°C and 25°C) and p CO 2 (400 and 1000 ppm) levels, utilizing global transcriptomic analysis. We find that evolutionary changes in the baseline gene expression, which we termed transcriptional up‐ and downregulation, is the primary mechanism used by diatoms to acclimate to the combined conditions of ocean warming and acidification. This transcriptional regulation shows that under higher temperature and p CO 2 conditions, photosynthesis, electron transport and carboxylation were modified with increasing abundances of genes encoding ATP, NADPH and carbon gaining for the carbon‐dioxide‐concentrating mechanisms (CCMs). Our results also indicate that changes in the transcriptional regulation of CCMs led to a decrease in the metabolic cost to save energy by promoting amino acid synthesis and nitrogen assimilation for the active protein processing machinery to adapt to warming and ocean acidification. This study generated unique metabolic insights into diatoms and suggests that future climate change conditions will cause evolutionary changes in oceanic diatoms that will facilitate their acclimation strategy. |
author2 |
Changjiang Scholar Program of Chinese Ministry of Education National Natural Science Foundation of China |
format |
Article in Journal/Newspaper |
author |
Thangaraj, Satheeswaran Sun, Jun |
spellingShingle |
Thangaraj, Satheeswaran Sun, Jun Transcriptomic reprogramming of the oceanic diatom Skeletonema dohrnii under warming ocean and acidification |
author_facet |
Thangaraj, Satheeswaran Sun, Jun |
author_sort |
Thangaraj, Satheeswaran |
title |
Transcriptomic reprogramming of the oceanic diatom Skeletonema dohrnii under warming ocean and acidification |
title_short |
Transcriptomic reprogramming of the oceanic diatom Skeletonema dohrnii under warming ocean and acidification |
title_full |
Transcriptomic reprogramming of the oceanic diatom Skeletonema dohrnii under warming ocean and acidification |
title_fullStr |
Transcriptomic reprogramming of the oceanic diatom Skeletonema dohrnii under warming ocean and acidification |
title_full_unstemmed |
Transcriptomic reprogramming of the oceanic diatom Skeletonema dohrnii under warming ocean and acidification |
title_sort |
transcriptomic reprogramming of the oceanic diatom skeletonema dohrnii under warming ocean and acidification |
publisher |
Wiley |
publishDate |
2020 |
url |
http://dx.doi.org/10.1111/1462-2920.15248 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.15248 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.15248 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Environmental Microbiology volume 23, issue 2, page 980-995 ISSN 1462-2912 1462-2920 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/1462-2920.15248 |
container_title |
Environmental Microbiology |
container_volume |
23 |
container_issue |
2 |
container_start_page |
980 |
op_container_end_page |
995 |
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1811643280794845184 |