Archaea in boreal Swedish lakes are diverse, dominated by Woesearchaeota and follow deterministic community assembly
Summary Despite their key role in biogeochemical processes, particularly the methane cycle, archaea are widely underrepresented in molecular surveys because of their lower abundance compared with bacteria and eukaryotes. Here, we use parallel high‐resolution small subunit rRNA gene sequencing to exp...
Published in: | Environmental Microbiology |
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Online Access: | http://dx.doi.org/10.1111/1462-2920.15058 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.15058 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.15058 |
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crwiley:10.1111/1462-2920.15058 2024-09-09T19:26:16+00:00 Archaea in boreal Swedish lakes are diverse, dominated by Woesearchaeota and follow deterministic community assembly Juottonen, Heli Fontaine, Laurent Wurzbacher, Christian Drakare, Stina Peura, Sari Eiler, Alexander Sveriges Lantbruksuniversitet Universitetet i Oslo Academy of Finland 2020 http://dx.doi.org/10.1111/1462-2920.15058 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.15058 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.15058 en eng Wiley http://creativecommons.org/licenses/by/4.0/ Environmental Microbiology volume 22, issue 8, page 3158-3171 ISSN 1462-2912 1462-2920 journal-article 2020 crwiley https://doi.org/10.1111/1462-2920.15058 2024-08-13T04:14:35Z Summary Despite their key role in biogeochemical processes, particularly the methane cycle, archaea are widely underrepresented in molecular surveys because of their lower abundance compared with bacteria and eukaryotes. Here, we use parallel high‐resolution small subunit rRNA gene sequencing to explore archaeal diversity in 109 Swedish lakes and correlate archaeal community assembly mechanisms to large‐scale latitudinal, climatic (nemoral to arctic) and nutrient (oligotrophic to eutrophic) gradients. Sequencing with universal primers showed the contribution of archaea was on average 0.8% but increased up to 1.5% of the three domains in forest lakes. Archaea‐specific sequencing revealed that freshwater archaeal diversity could be partly explained by lake variables associated with nutrient status. Combined with deterministic co‐occurrence patterns this finding suggests that ecological drift is overridden by environmental sorting, as well as other deterministic processes such as biogeographic and evolutionary history, leading to lake‐specific archaeal biodiversity. Acetoclastic, hydrogenotrophic and methylotrophic methanogens as well as ammonia‐oxidizing archaea were frequently detected across the lakes. Archaea‐specific sequencing also revealed representatives of Woesearchaeota and other phyla of the DPANN superphylum. This study adds to our understanding of the ecological range of key archaea in freshwaters and links these taxa to hypotheses about processes governing biogeochemical cycles in lakes. Article in Journal/Newspaper Arctic Wiley Online Library Arctic Environmental Microbiology 22 8 3158 3171 |
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Wiley Online Library |
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crwiley |
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English |
description |
Summary Despite their key role in biogeochemical processes, particularly the methane cycle, archaea are widely underrepresented in molecular surveys because of their lower abundance compared with bacteria and eukaryotes. Here, we use parallel high‐resolution small subunit rRNA gene sequencing to explore archaeal diversity in 109 Swedish lakes and correlate archaeal community assembly mechanisms to large‐scale latitudinal, climatic (nemoral to arctic) and nutrient (oligotrophic to eutrophic) gradients. Sequencing with universal primers showed the contribution of archaea was on average 0.8% but increased up to 1.5% of the three domains in forest lakes. Archaea‐specific sequencing revealed that freshwater archaeal diversity could be partly explained by lake variables associated with nutrient status. Combined with deterministic co‐occurrence patterns this finding suggests that ecological drift is overridden by environmental sorting, as well as other deterministic processes such as biogeographic and evolutionary history, leading to lake‐specific archaeal biodiversity. Acetoclastic, hydrogenotrophic and methylotrophic methanogens as well as ammonia‐oxidizing archaea were frequently detected across the lakes. Archaea‐specific sequencing also revealed representatives of Woesearchaeota and other phyla of the DPANN superphylum. This study adds to our understanding of the ecological range of key archaea in freshwaters and links these taxa to hypotheses about processes governing biogeochemical cycles in lakes. |
author2 |
Sveriges Lantbruksuniversitet Universitetet i Oslo Academy of Finland |
format |
Article in Journal/Newspaper |
author |
Juottonen, Heli Fontaine, Laurent Wurzbacher, Christian Drakare, Stina Peura, Sari Eiler, Alexander |
spellingShingle |
Juottonen, Heli Fontaine, Laurent Wurzbacher, Christian Drakare, Stina Peura, Sari Eiler, Alexander Archaea in boreal Swedish lakes are diverse, dominated by Woesearchaeota and follow deterministic community assembly |
author_facet |
Juottonen, Heli Fontaine, Laurent Wurzbacher, Christian Drakare, Stina Peura, Sari Eiler, Alexander |
author_sort |
Juottonen, Heli |
title |
Archaea in boreal Swedish lakes are diverse, dominated by Woesearchaeota and follow deterministic community assembly |
title_short |
Archaea in boreal Swedish lakes are diverse, dominated by Woesearchaeota and follow deterministic community assembly |
title_full |
Archaea in boreal Swedish lakes are diverse, dominated by Woesearchaeota and follow deterministic community assembly |
title_fullStr |
Archaea in boreal Swedish lakes are diverse, dominated by Woesearchaeota and follow deterministic community assembly |
title_full_unstemmed |
Archaea in boreal Swedish lakes are diverse, dominated by Woesearchaeota and follow deterministic community assembly |
title_sort |
archaea in boreal swedish lakes are diverse, dominated by woesearchaeota and follow deterministic community assembly |
publisher |
Wiley |
publishDate |
2020 |
url |
http://dx.doi.org/10.1111/1462-2920.15058 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.15058 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.15058 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Environmental Microbiology volume 22, issue 8, page 3158-3171 ISSN 1462-2912 1462-2920 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1111/1462-2920.15058 |
container_title |
Environmental Microbiology |
container_volume |
22 |
container_issue |
8 |
container_start_page |
3158 |
op_container_end_page |
3171 |
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1809895923910705152 |