Protein expression in the obligate hydrocarbon‐degrading psychrophile Oleispira antarctica RB‐8 during alkane degradation and cold tolerance
Summary In cold marine environments, the obligate hydrocarbon‐degrading psychrophile Oleispira antarctica RB‐8, which utilizes aliphatic alkanes almost exclusively as substrates, dominates microbial communities following oil spills. In this study, LC–MS/MS shotgun proteomics was used to identify cha...
Published in: | Environmental Microbiology |
---|---|
Main Authors: | , , , , |
Other Authors: | , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2020
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1111/1462-2920.14956 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1462-2920.14956 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.14956 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.14956 |
id |
crwiley:10.1111/1462-2920.14956 |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1111/1462-2920.14956 2024-06-23T07:46:06+00:00 Protein expression in the obligate hydrocarbon‐degrading psychrophile Oleispira antarctica RB‐8 during alkane degradation and cold tolerance Gregson, Benjamin H. Metodieva, Gergana Metodiev, Metodi V. Golyshin, Peter N. McKew, Boyd A. Horizon 2020 Framework Programme Natural Environment Research Council 2020 http://dx.doi.org/10.1111/1462-2920.14956 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1462-2920.14956 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.14956 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.14956 en eng Wiley http://creativecommons.org/licenses/by/4.0/ Environmental Microbiology volume 22, issue 5, page 1870-1883 ISSN 1462-2912 1462-2920 journal-article 2020 crwiley https://doi.org/10.1111/1462-2920.14956 2024-06-06T04:24:18Z Summary In cold marine environments, the obligate hydrocarbon‐degrading psychrophile Oleispira antarctica RB‐8, which utilizes aliphatic alkanes almost exclusively as substrates, dominates microbial communities following oil spills. In this study, LC–MS/MS shotgun proteomics was used to identify changes in the proteome induced during growth on n ‐alkanes and in cold temperatures. Specifically, proteins with significantly higher relative abundance during growth on tetradecane ( n ‐C 14 ) at 16°C and 4°C have been quantified. During growth on n ‐C 14 , O . antarctica expressed a complete pathway for the terminal oxidation of n ‐alkanes including two alkane monooxygenases, two alcohol dehydrogenases, two aldehyde dehydrogenases, a fatty‐acid‐CoA ligase, a fatty acid desaturase and associated oxidoreductases. Increased biosynthesis of these proteins ranged from 3‐ to 21‐fold compared with growth on a non‐hydrocarbon control. This study also highlights mechanisms O . antarctica may utilize to provide it with ecological competitiveness at low temperatures. This was evidenced by an increase in spectral counts for proteins involved in flagella structure/output to overcome higher viscosity, flagella rotation to accumulate cells and proline metabolism to counteract oxidative stress, during growth at 4°C compared with 16°C. Such species‐specific understanding of the physiology during hydrocarbon degradation can be important for parameterizing models that predict the fate of marine oil spills. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Environmental Microbiology 22 5 1870 1883 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Summary In cold marine environments, the obligate hydrocarbon‐degrading psychrophile Oleispira antarctica RB‐8, which utilizes aliphatic alkanes almost exclusively as substrates, dominates microbial communities following oil spills. In this study, LC–MS/MS shotgun proteomics was used to identify changes in the proteome induced during growth on n ‐alkanes and in cold temperatures. Specifically, proteins with significantly higher relative abundance during growth on tetradecane ( n ‐C 14 ) at 16°C and 4°C have been quantified. During growth on n ‐C 14 , O . antarctica expressed a complete pathway for the terminal oxidation of n ‐alkanes including two alkane monooxygenases, two alcohol dehydrogenases, two aldehyde dehydrogenases, a fatty‐acid‐CoA ligase, a fatty acid desaturase and associated oxidoreductases. Increased biosynthesis of these proteins ranged from 3‐ to 21‐fold compared with growth on a non‐hydrocarbon control. This study also highlights mechanisms O . antarctica may utilize to provide it with ecological competitiveness at low temperatures. This was evidenced by an increase in spectral counts for proteins involved in flagella structure/output to overcome higher viscosity, flagella rotation to accumulate cells and proline metabolism to counteract oxidative stress, during growth at 4°C compared with 16°C. Such species‐specific understanding of the physiology during hydrocarbon degradation can be important for parameterizing models that predict the fate of marine oil spills. |
author2 |
Horizon 2020 Framework Programme Natural Environment Research Council |
format |
Article in Journal/Newspaper |
author |
Gregson, Benjamin H. Metodieva, Gergana Metodiev, Metodi V. Golyshin, Peter N. McKew, Boyd A. |
spellingShingle |
Gregson, Benjamin H. Metodieva, Gergana Metodiev, Metodi V. Golyshin, Peter N. McKew, Boyd A. Protein expression in the obligate hydrocarbon‐degrading psychrophile Oleispira antarctica RB‐8 during alkane degradation and cold tolerance |
author_facet |
Gregson, Benjamin H. Metodieva, Gergana Metodiev, Metodi V. Golyshin, Peter N. McKew, Boyd A. |
author_sort |
Gregson, Benjamin H. |
title |
Protein expression in the obligate hydrocarbon‐degrading psychrophile Oleispira antarctica RB‐8 during alkane degradation and cold tolerance |
title_short |
Protein expression in the obligate hydrocarbon‐degrading psychrophile Oleispira antarctica RB‐8 during alkane degradation and cold tolerance |
title_full |
Protein expression in the obligate hydrocarbon‐degrading psychrophile Oleispira antarctica RB‐8 during alkane degradation and cold tolerance |
title_fullStr |
Protein expression in the obligate hydrocarbon‐degrading psychrophile Oleispira antarctica RB‐8 during alkane degradation and cold tolerance |
title_full_unstemmed |
Protein expression in the obligate hydrocarbon‐degrading psychrophile Oleispira antarctica RB‐8 during alkane degradation and cold tolerance |
title_sort |
protein expression in the obligate hydrocarbon‐degrading psychrophile oleispira antarctica rb‐8 during alkane degradation and cold tolerance |
publisher |
Wiley |
publishDate |
2020 |
url |
http://dx.doi.org/10.1111/1462-2920.14956 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1462-2920.14956 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.14956 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.14956 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Environmental Microbiology volume 22, issue 5, page 1870-1883 ISSN 1462-2912 1462-2920 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1111/1462-2920.14956 |
container_title |
Environmental Microbiology |
container_volume |
22 |
container_issue |
5 |
container_start_page |
1870 |
op_container_end_page |
1883 |
_version_ |
1802643931855323136 |