Selection of Vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells
Summary Pacific oyster mortality syndrome affects juveniles of Crassostrea gigas oysters and threatens the sustainability of commercial and natural stocks of this species. Vibrio crassostreae ( V . crassostreae ) has been repeatedly isolated from diseased animals, and the majority of the strains hav...
Published in: | Environmental Microbiology |
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Online Access: | http://dx.doi.org/10.1111/1462-2920.14776 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.14776 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.14776 |
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crwiley:10.1111/1462-2920.14776 2024-09-30T14:33:58+00:00 Selection of Vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells Piel, Damien Bruto, Maxime James, Adèle Labreuche, Yannick Lambert, Christophe Janicot, Adrian Chenivesse, Sabine Petton, Bruno Wegner, K. Mathias Stoudmann, Candice Blokesch, Melanie Le Roux, Frédérique Agence Nationale de la Recherche European Research Council Howard Hughes Medical Institute Swiss National Science Foundation 2019 http://dx.doi.org/10.1111/1462-2920.14776 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.14776 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.14776 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Microbiology volume 22, issue 10, page 4198-4211 ISSN 1462-2912 1462-2920 journal-article 2019 crwiley https://doi.org/10.1111/1462-2920.14776 2024-09-05T05:10:46Z Summary Pacific oyster mortality syndrome affects juveniles of Crassostrea gigas oysters and threatens the sustainability of commercial and natural stocks of this species. Vibrio crassostreae ( V . crassostreae ) has been repeatedly isolated from diseased animals, and the majority of the strains have been demonstrated to be virulent for oysters. In this study, we showed that oyster farms exhibited a high prevalence of a virulence plasmid carried by V . crassostreae , while oysters, at an adult stage, were reservoirs of this virulent population. The pathogenicity of V . crassostreae depends on a novel transcriptional regulator, which activates the bidirectional promoter of a type 6 secretion system (T6SS) genes cluster. Both the T6SS and a second chromosomal virulence factor, r5 . 7 , are necessary for virulence but act independently to cause haemocyte (oyster immune cell) cytotoxicity. A phylogenetically closely related T6SS was identified in V . aestuarianus and V . tapetis , which infect adult oysters and clams respectively. We propose that haemocyte cytotoxicity is a lethality trait shared by a broad range of mollusc pathogens, and we speculate that T6SS was involved in parallel evolution of pathogen for molluscs. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Wiley Online Library Pacific Environmental Microbiology 22 10 4198 4211 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Summary Pacific oyster mortality syndrome affects juveniles of Crassostrea gigas oysters and threatens the sustainability of commercial and natural stocks of this species. Vibrio crassostreae ( V . crassostreae ) has been repeatedly isolated from diseased animals, and the majority of the strains have been demonstrated to be virulent for oysters. In this study, we showed that oyster farms exhibited a high prevalence of a virulence plasmid carried by V . crassostreae , while oysters, at an adult stage, were reservoirs of this virulent population. The pathogenicity of V . crassostreae depends on a novel transcriptional regulator, which activates the bidirectional promoter of a type 6 secretion system (T6SS) genes cluster. Both the T6SS and a second chromosomal virulence factor, r5 . 7 , are necessary for virulence but act independently to cause haemocyte (oyster immune cell) cytotoxicity. A phylogenetically closely related T6SS was identified in V . aestuarianus and V . tapetis , which infect adult oysters and clams respectively. We propose that haemocyte cytotoxicity is a lethality trait shared by a broad range of mollusc pathogens, and we speculate that T6SS was involved in parallel evolution of pathogen for molluscs. |
author2 |
Agence Nationale de la Recherche European Research Council Howard Hughes Medical Institute Swiss National Science Foundation |
format |
Article in Journal/Newspaper |
author |
Piel, Damien Bruto, Maxime James, Adèle Labreuche, Yannick Lambert, Christophe Janicot, Adrian Chenivesse, Sabine Petton, Bruno Wegner, K. Mathias Stoudmann, Candice Blokesch, Melanie Le Roux, Frédérique |
spellingShingle |
Piel, Damien Bruto, Maxime James, Adèle Labreuche, Yannick Lambert, Christophe Janicot, Adrian Chenivesse, Sabine Petton, Bruno Wegner, K. Mathias Stoudmann, Candice Blokesch, Melanie Le Roux, Frédérique Selection of Vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells |
author_facet |
Piel, Damien Bruto, Maxime James, Adèle Labreuche, Yannick Lambert, Christophe Janicot, Adrian Chenivesse, Sabine Petton, Bruno Wegner, K. Mathias Stoudmann, Candice Blokesch, Melanie Le Roux, Frédérique |
author_sort |
Piel, Damien |
title |
Selection of Vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells |
title_short |
Selection of Vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells |
title_full |
Selection of Vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells |
title_fullStr |
Selection of Vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells |
title_full_unstemmed |
Selection of Vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells |
title_sort |
selection of vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells |
publisher |
Wiley |
publishDate |
2019 |
url |
http://dx.doi.org/10.1111/1462-2920.14776 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.14776 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.14776 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Environmental Microbiology volume 22, issue 10, page 4198-4211 ISSN 1462-2912 1462-2920 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/1462-2920.14776 |
container_title |
Environmental Microbiology |
container_volume |
22 |
container_issue |
10 |
container_start_page |
4198 |
op_container_end_page |
4211 |
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1811637710796881920 |