Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment next‐generation sequencing strategy
Summary Infectious agents such as the bacteria Vibrio aestuarianus or Ostreid herpesvirus 1 have been repeatedly associated with dramatic disease outbreaks of Crassostrea gigas beds in Europe. Beside roles played by these pathogens, microbial infections in C . gigas may derive from the contribution...
Published in: | Environmental Microbiology |
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Online Access: | http://dx.doi.org/10.1111/1462-2920.14750 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1462-2920.14750 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.14750 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.14750 |
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crwiley:10.1111/1462-2920.14750 2024-10-06T13:48:07+00:00 Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment next‐generation sequencing strategy Lasa, Aide di Cesare, Andrea Tassistro, Giovanni Borello, Alessio Gualdi, Stefano Furones, Dolors Carrasco, Noelia Cheslett, Deborah Brechon, Amanda Paillard, Christine Bidault, Adeline Pernet, Fabrice Canesi, Laura Edomi, Paolo Pallavicini, Alberto Pruzzo, Carla Vezzulli, Luigi H2020 Food 2019 http://dx.doi.org/10.1111/1462-2920.14750 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1462-2920.14750 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.14750 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.14750 en eng Wiley http://creativecommons.org/licenses/by/4.0/ Environmental Microbiology volume 21, issue 12, page 4548-4562 ISSN 1462-2912 1462-2920 journal-article 2019 crwiley https://doi.org/10.1111/1462-2920.14750 2024-09-11T04:11:58Z Summary Infectious agents such as the bacteria Vibrio aestuarianus or Ostreid herpesvirus 1 have been repeatedly associated with dramatic disease outbreaks of Crassostrea gigas beds in Europe. Beside roles played by these pathogens, microbial infections in C . gigas may derive from the contribution of a larger number of microorganisms than previously thought, according to an emerging view supporting the polymicrobial nature of bivalve diseases. In this study, the microbial communities associated with a large number of C . gigas samples collected during recurrent mortality episodes at different European sites were investigated by real‐time PCR and 16SrRNA gene‐based microbial profiling. A new target enrichment next‐generation sequencing protocol for selective capturing of 884 phylogenetic and virulence markers of the potential microbial pathogenic community in oyster tissue was developed allowing high taxonomic resolution analysis of the bivalve pathobiota. Comparative analysis of contrasting C . gigas samples conducted using these methods revealed that oyster experiencing mortality outbreaks displayed signs of microbiota disruption associated with the presence of previously undetected potential pathogenic microbial species mostly belonging to genus Vibrio and Arcobacter . The role of these species and their consortia should be targeted by future studies aiming to shed light on mechanisms underlying polymicrobial infections in C . gigas . Article in Journal/Newspaper Crassostrea gigas Pacific oyster Wiley Online Library Pacific Environmental Microbiology 21 12 4548 4562 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Summary Infectious agents such as the bacteria Vibrio aestuarianus or Ostreid herpesvirus 1 have been repeatedly associated with dramatic disease outbreaks of Crassostrea gigas beds in Europe. Beside roles played by these pathogens, microbial infections in C . gigas may derive from the contribution of a larger number of microorganisms than previously thought, according to an emerging view supporting the polymicrobial nature of bivalve diseases. In this study, the microbial communities associated with a large number of C . gigas samples collected during recurrent mortality episodes at different European sites were investigated by real‐time PCR and 16SrRNA gene‐based microbial profiling. A new target enrichment next‐generation sequencing protocol for selective capturing of 884 phylogenetic and virulence markers of the potential microbial pathogenic community in oyster tissue was developed allowing high taxonomic resolution analysis of the bivalve pathobiota. Comparative analysis of contrasting C . gigas samples conducted using these methods revealed that oyster experiencing mortality outbreaks displayed signs of microbiota disruption associated with the presence of previously undetected potential pathogenic microbial species mostly belonging to genus Vibrio and Arcobacter . The role of these species and their consortia should be targeted by future studies aiming to shed light on mechanisms underlying polymicrobial infections in C . gigas . |
author2 |
H2020 Food |
format |
Article in Journal/Newspaper |
author |
Lasa, Aide di Cesare, Andrea Tassistro, Giovanni Borello, Alessio Gualdi, Stefano Furones, Dolors Carrasco, Noelia Cheslett, Deborah Brechon, Amanda Paillard, Christine Bidault, Adeline Pernet, Fabrice Canesi, Laura Edomi, Paolo Pallavicini, Alberto Pruzzo, Carla Vezzulli, Luigi |
spellingShingle |
Lasa, Aide di Cesare, Andrea Tassistro, Giovanni Borello, Alessio Gualdi, Stefano Furones, Dolors Carrasco, Noelia Cheslett, Deborah Brechon, Amanda Paillard, Christine Bidault, Adeline Pernet, Fabrice Canesi, Laura Edomi, Paolo Pallavicini, Alberto Pruzzo, Carla Vezzulli, Luigi Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment next‐generation sequencing strategy |
author_facet |
Lasa, Aide di Cesare, Andrea Tassistro, Giovanni Borello, Alessio Gualdi, Stefano Furones, Dolors Carrasco, Noelia Cheslett, Deborah Brechon, Amanda Paillard, Christine Bidault, Adeline Pernet, Fabrice Canesi, Laura Edomi, Paolo Pallavicini, Alberto Pruzzo, Carla Vezzulli, Luigi |
author_sort |
Lasa, Aide |
title |
Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment next‐generation sequencing strategy |
title_short |
Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment next‐generation sequencing strategy |
title_full |
Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment next‐generation sequencing strategy |
title_fullStr |
Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment next‐generation sequencing strategy |
title_full_unstemmed |
Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment next‐generation sequencing strategy |
title_sort |
dynamics of the pacific oyster pathobiota during mortality episodes in europe assessed by 16s rrna gene profiling and a new target enrichment next‐generation sequencing strategy |
publisher |
Wiley |
publishDate |
2019 |
url |
http://dx.doi.org/10.1111/1462-2920.14750 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1462-2920.14750 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.14750 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.14750 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Environmental Microbiology volume 21, issue 12, page 4548-4562 ISSN 1462-2912 1462-2920 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1111/1462-2920.14750 |
container_title |
Environmental Microbiology |
container_volume |
21 |
container_issue |
12 |
container_start_page |
4548 |
op_container_end_page |
4562 |
_version_ |
1812176278160146432 |