Biochemical diversity of glycosphingolipid biosynthesis as a driver of Coccolithovirus competitive ecology
Summary Coccolithoviruses (EhVs) are large, double‐stranded DNA‐containing viruses that infect the single‐celled, marine coccolithophore Emiliania huxleyi . Given the cosmopolitan nature and global importance of E. huxleyi as a bloom‐forming, calcifying, photoautotroph, E. huxleyi –EhV interactions...
Published in: | Environmental Microbiology |
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Online Access: | http://dx.doi.org/10.1111/1462-2920.14633 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1462-2920.14633 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.14633 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.14633 https://sfamjournals.onlinelibrary.wiley.com/doi/am-pdf/10.1111/1462-2920.14633 |
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crwiley:10.1111/1462-2920.14633 2024-06-02T08:11:14+00:00 Biochemical diversity of glycosphingolipid biosynthesis as a driver of Coccolithovirus competitive ecology Nissimov, Jozef I. Talmy, David Haramaty, Liti Fredricks, Helen F. Zelzion, Ehud Knowles, Ben Eren, A. Murat Vandzura, Rebecca Laber, Christien P. Schieler, Brittany M. Johns, Christopher T. More, Kuldeep D. Coolen, Marco J. L. Follows, Michael J. Bhattacharya, Debashish Van Mooy, Benjamin A. S. Bidle, Kay D. National Science Foundation Gordon and Betty Moore Foundation Gordon and Betty Moore Foundation 2019 http://dx.doi.org/10.1111/1462-2920.14633 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1462-2920.14633 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.14633 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.14633 https://sfamjournals.onlinelibrary.wiley.com/doi/am-pdf/10.1111/1462-2920.14633 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#am http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Microbiology volume 21, issue 6, page 2182-2197 ISSN 1462-2912 1462-2920 journal-article 2019 crwiley https://doi.org/10.1111/1462-2920.14633 2024-05-03T11:05:12Z Summary Coccolithoviruses (EhVs) are large, double‐stranded DNA‐containing viruses that infect the single‐celled, marine coccolithophore Emiliania huxleyi . Given the cosmopolitan nature and global importance of E. huxleyi as a bloom‐forming, calcifying, photoautotroph, E. huxleyi –EhV interactions play a key role in oceanic carbon biogeochemistry. Virally‐encoded glycosphingolipids (vGSLs) are virulence factors that are produced by the activity of virus‐encoded serine palmitoyltransferase (SPT). Here, we characterize the dynamics, diversity and catalytic production of vGSLs in an array of EhV strains in relation to their SPT sequence composition and explore the hypothesis that they are a determinant of infectivity and host demise. vGSL production and diversity was positively correlated with increased virulence, virus replication rate and lytic infection dynamics in laboratory experiments, but they do not explain the success of less‐virulent EhVs in natural EhV communities. The majority of EhV‐derived SPT amplicon sequences associated with infected cells in the North Atlantic derived from slower infecting, less virulent EhVs. Our lab‐, field‐ and mathematical model‐based data and simulations support ecological scenarios whereby slow‐infecting, less‐virulent EhVs successfully compete in North Atlantic populations of E. huxleyi , through either the preferential removal of fast‐infecting, virulent EhVs during active infection or by having access to a broader host range. Article in Journal/Newspaper North Atlantic Wiley Online Library Environmental Microbiology 21 6 2182 2197 |
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Wiley Online Library |
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English |
description |
Summary Coccolithoviruses (EhVs) are large, double‐stranded DNA‐containing viruses that infect the single‐celled, marine coccolithophore Emiliania huxleyi . Given the cosmopolitan nature and global importance of E. huxleyi as a bloom‐forming, calcifying, photoautotroph, E. huxleyi –EhV interactions play a key role in oceanic carbon biogeochemistry. Virally‐encoded glycosphingolipids (vGSLs) are virulence factors that are produced by the activity of virus‐encoded serine palmitoyltransferase (SPT). Here, we characterize the dynamics, diversity and catalytic production of vGSLs in an array of EhV strains in relation to their SPT sequence composition and explore the hypothesis that they are a determinant of infectivity and host demise. vGSL production and diversity was positively correlated with increased virulence, virus replication rate and lytic infection dynamics in laboratory experiments, but they do not explain the success of less‐virulent EhVs in natural EhV communities. The majority of EhV‐derived SPT amplicon sequences associated with infected cells in the North Atlantic derived from slower infecting, less virulent EhVs. Our lab‐, field‐ and mathematical model‐based data and simulations support ecological scenarios whereby slow‐infecting, less‐virulent EhVs successfully compete in North Atlantic populations of E. huxleyi , through either the preferential removal of fast‐infecting, virulent EhVs during active infection or by having access to a broader host range. |
author2 |
National Science Foundation Gordon and Betty Moore Foundation Gordon and Betty Moore Foundation |
format |
Article in Journal/Newspaper |
author |
Nissimov, Jozef I. Talmy, David Haramaty, Liti Fredricks, Helen F. Zelzion, Ehud Knowles, Ben Eren, A. Murat Vandzura, Rebecca Laber, Christien P. Schieler, Brittany M. Johns, Christopher T. More, Kuldeep D. Coolen, Marco J. L. Follows, Michael J. Bhattacharya, Debashish Van Mooy, Benjamin A. S. Bidle, Kay D. |
spellingShingle |
Nissimov, Jozef I. Talmy, David Haramaty, Liti Fredricks, Helen F. Zelzion, Ehud Knowles, Ben Eren, A. Murat Vandzura, Rebecca Laber, Christien P. Schieler, Brittany M. Johns, Christopher T. More, Kuldeep D. Coolen, Marco J. L. Follows, Michael J. Bhattacharya, Debashish Van Mooy, Benjamin A. S. Bidle, Kay D. Biochemical diversity of glycosphingolipid biosynthesis as a driver of Coccolithovirus competitive ecology |
author_facet |
Nissimov, Jozef I. Talmy, David Haramaty, Liti Fredricks, Helen F. Zelzion, Ehud Knowles, Ben Eren, A. Murat Vandzura, Rebecca Laber, Christien P. Schieler, Brittany M. Johns, Christopher T. More, Kuldeep D. Coolen, Marco J. L. Follows, Michael J. Bhattacharya, Debashish Van Mooy, Benjamin A. S. Bidle, Kay D. |
author_sort |
Nissimov, Jozef I. |
title |
Biochemical diversity of glycosphingolipid biosynthesis as a driver of Coccolithovirus competitive ecology |
title_short |
Biochemical diversity of glycosphingolipid biosynthesis as a driver of Coccolithovirus competitive ecology |
title_full |
Biochemical diversity of glycosphingolipid biosynthesis as a driver of Coccolithovirus competitive ecology |
title_fullStr |
Biochemical diversity of glycosphingolipid biosynthesis as a driver of Coccolithovirus competitive ecology |
title_full_unstemmed |
Biochemical diversity of glycosphingolipid biosynthesis as a driver of Coccolithovirus competitive ecology |
title_sort |
biochemical diversity of glycosphingolipid biosynthesis as a driver of coccolithovirus competitive ecology |
publisher |
Wiley |
publishDate |
2019 |
url |
http://dx.doi.org/10.1111/1462-2920.14633 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1462-2920.14633 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.14633 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.14633 https://sfamjournals.onlinelibrary.wiley.com/doi/am-pdf/10.1111/1462-2920.14633 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Environmental Microbiology volume 21, issue 6, page 2182-2197 ISSN 1462-2912 1462-2920 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#am http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/1462-2920.14633 |
container_title |
Environmental Microbiology |
container_volume |
21 |
container_issue |
6 |
container_start_page |
2182 |
op_container_end_page |
2197 |
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1800757301693382656 |