Microbial iron metabolism as revealed by gene expression profiles in contrasted Southern Ocean regimes
Summary Iron (Fe) is a limiting nutrient in large regions of the ocean, but the strategies of prokaryotes to cope with this micronutrient are poorly known. Using a gene‐specific approach from metatranscriptomics data, we investigated seven Fe‐related metabolic pathways in microbial communities from...
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crwiley:10.1111/1462-2920.14621 2024-06-02T08:14:43+00:00 Microbial iron metabolism as revealed by gene expression profiles in contrasted Southern Ocean regimes Debeljak, Pavla Toulza, Eve Beier, Sara Blain, Stephane Obernosterer, Ingrid Agence Nationale de la Recherche Austrian Science Fund 2019 http://dx.doi.org/10.1111/1462-2920.14621 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.14621 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.14621 en eng Wiley http://creativecommons.org/licenses/by/4.0/ Environmental Microbiology volume 21, issue 7, page 2360-2374 ISSN 1462-2912 1462-2920 journal-article 2019 crwiley https://doi.org/10.1111/1462-2920.14621 2024-05-06T07:00:46Z Summary Iron (Fe) is a limiting nutrient in large regions of the ocean, but the strategies of prokaryotes to cope with this micronutrient are poorly known. Using a gene‐specific approach from metatranscriptomics data, we investigated seven Fe‐related metabolic pathways in microbial communities from high nutrient low chlorophyll and naturally Fe‐fertilized waters in the Southern Ocean. We observed major differences in the contribution of prokaryotic groups at different taxonomic levels to transcripts encoding Fe‐uptake mechanisms, intracellular Fe storage and replacement and Fe‐related pathways in the tricarboxylic acid (TCA) cycle. The composition of the prokaryotic communities contributing to the transcripts of a given Fe‐related pathway was overall independent of the in situ Fe supply, indicating that microbial taxa utilize distinct Fe‐related metabolic processes. Only a few prokaryotic groups contributed to the transcripts of more than one Fe‐uptake mechanism, suggesting limited metabolic versatility. Taxa‐specific expression of individual genes varied among prokaryotic groups and was substantially higher for all inspected genes in Fe‐limited as compared to naturally fertilized waters, indicating the link between transcriptional state and Fe regime. Different metabolic strategies regarding low Fe concentrations in the Southern Ocean are discussed for two abundant prokaryotic groups, Pelagibacteraceae and Flavobacteriaceae . Article in Journal/Newspaper Southern Ocean Wiley Online Library Southern Ocean Environmental Microbiology 21 7 2360 2374 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Summary Iron (Fe) is a limiting nutrient in large regions of the ocean, but the strategies of prokaryotes to cope with this micronutrient are poorly known. Using a gene‐specific approach from metatranscriptomics data, we investigated seven Fe‐related metabolic pathways in microbial communities from high nutrient low chlorophyll and naturally Fe‐fertilized waters in the Southern Ocean. We observed major differences in the contribution of prokaryotic groups at different taxonomic levels to transcripts encoding Fe‐uptake mechanisms, intracellular Fe storage and replacement and Fe‐related pathways in the tricarboxylic acid (TCA) cycle. The composition of the prokaryotic communities contributing to the transcripts of a given Fe‐related pathway was overall independent of the in situ Fe supply, indicating that microbial taxa utilize distinct Fe‐related metabolic processes. Only a few prokaryotic groups contributed to the transcripts of more than one Fe‐uptake mechanism, suggesting limited metabolic versatility. Taxa‐specific expression of individual genes varied among prokaryotic groups and was substantially higher for all inspected genes in Fe‐limited as compared to naturally fertilized waters, indicating the link between transcriptional state and Fe regime. Different metabolic strategies regarding low Fe concentrations in the Southern Ocean are discussed for two abundant prokaryotic groups, Pelagibacteraceae and Flavobacteriaceae . |
author2 |
Agence Nationale de la Recherche Austrian Science Fund |
format |
Article in Journal/Newspaper |
author |
Debeljak, Pavla Toulza, Eve Beier, Sara Blain, Stephane Obernosterer, Ingrid |
spellingShingle |
Debeljak, Pavla Toulza, Eve Beier, Sara Blain, Stephane Obernosterer, Ingrid Microbial iron metabolism as revealed by gene expression profiles in contrasted Southern Ocean regimes |
author_facet |
Debeljak, Pavla Toulza, Eve Beier, Sara Blain, Stephane Obernosterer, Ingrid |
author_sort |
Debeljak, Pavla |
title |
Microbial iron metabolism as revealed by gene expression profiles in contrasted Southern Ocean regimes |
title_short |
Microbial iron metabolism as revealed by gene expression profiles in contrasted Southern Ocean regimes |
title_full |
Microbial iron metabolism as revealed by gene expression profiles in contrasted Southern Ocean regimes |
title_fullStr |
Microbial iron metabolism as revealed by gene expression profiles in contrasted Southern Ocean regimes |
title_full_unstemmed |
Microbial iron metabolism as revealed by gene expression profiles in contrasted Southern Ocean regimes |
title_sort |
microbial iron metabolism as revealed by gene expression profiles in contrasted southern ocean regimes |
publisher |
Wiley |
publishDate |
2019 |
url |
http://dx.doi.org/10.1111/1462-2920.14621 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.14621 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.14621 |
geographic |
Southern Ocean |
geographic_facet |
Southern Ocean |
genre |
Southern Ocean |
genre_facet |
Southern Ocean |
op_source |
Environmental Microbiology volume 21, issue 7, page 2360-2374 ISSN 1462-2912 1462-2920 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1111/1462-2920.14621 |
container_title |
Environmental Microbiology |
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21 |
container_issue |
7 |
container_start_page |
2360 |
op_container_end_page |
2374 |
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1800738685493182464 |