Oxidation of urea‐derived nitrogen by thaumarchaeota‐dominated marine nitrifying communities
Summary Urea nitrogen has been proposed to contribute significantly to nitrification by marine thaumarchaeotes. These inferences are based on distributions of thaumarchaeote urease genes rather than activity measurements. We found that ammonia oxidation rates were always higher than oxidation rates...
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crwiley:10.1111/1462-2920.13457 2024-09-15T17:44:50+00:00 Oxidation of urea‐derived nitrogen by thaumarchaeota‐dominated marine nitrifying communities Tolar, Bradley B. Wallsgrove, Natalie J. Popp, Brian N. Hollibaugh, James T. Division of Ocean Sciences 2016 http://dx.doi.org/10.1111/1462-2920.13457 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1462-2920.13457 http://api.wiley.com/onlinelibrary/chorus/v1/articles/10.1111%2F1462-2920.13457 https://sfamjournals.onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.13457 http://onlinelibrary.wiley.com/wol1/doi/10.1111/1462-2920.13457/fullpdf en eng Wiley http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ Environmental Microbiology volume 19, issue 12, page 4838-4850 ISSN 1462-2912 1462-2920 journal-article 2016 crwiley https://doi.org/10.1111/1462-2920.13457 2024-08-27T04:28:45Z Summary Urea nitrogen has been proposed to contribute significantly to nitrification by marine thaumarchaeotes. These inferences are based on distributions of thaumarchaeote urease genes rather than activity measurements. We found that ammonia oxidation rates were always higher than oxidation rates of urea‐derived N in samples from coastal Georgia, USA (means ± SEM: 382 ± 35 versus 73 ± 24 nmol L −1 d −1 , Mann‐Whitney U‐test p < 0.0001), and the South Atlantic Bight (20 ± 8.8 versus 2.2 ± 1.7 nmol L −1 d −1 , p = 0.026) but not the Gulf of Alaska (8.8 ± 4.0 versus 1.5 ± 0.6, p > 0.05). Urea‐derived N was relatively more important in samples from Antarctic continental shelf waters, though the difference was not statistically significant (19.4 ± 4.8 versus 12.0 ± 2.7 nmol L −1 d −1 , p > 0.05). We found only weak correlations between oxidation rates of urea‐derived N and the abundance or transcription of putative Thaumarchaeota ureC genes. Dependence on urea‐derived N does not appear to be directly related to pH or ammonium concentrations. Competition experiments and release of 15 NH 3 suggest that urea is hydrolyzed to ammonia intracellularly, then a portion is lost to the dissolved pool. The contribution of urea‐derived N to nitrification appears to be minor in temperate coastal waters, but may represent a significant portion of the nitrification flux in Antarctic coastal waters. Article in Journal/Newspaper Antarc* Antarctic Alaska Wiley Online Library Environmental Microbiology 19 12 4838 4850 |
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Wiley Online Library |
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English |
description |
Summary Urea nitrogen has been proposed to contribute significantly to nitrification by marine thaumarchaeotes. These inferences are based on distributions of thaumarchaeote urease genes rather than activity measurements. We found that ammonia oxidation rates were always higher than oxidation rates of urea‐derived N in samples from coastal Georgia, USA (means ± SEM: 382 ± 35 versus 73 ± 24 nmol L −1 d −1 , Mann‐Whitney U‐test p < 0.0001), and the South Atlantic Bight (20 ± 8.8 versus 2.2 ± 1.7 nmol L −1 d −1 , p = 0.026) but not the Gulf of Alaska (8.8 ± 4.0 versus 1.5 ± 0.6, p > 0.05). Urea‐derived N was relatively more important in samples from Antarctic continental shelf waters, though the difference was not statistically significant (19.4 ± 4.8 versus 12.0 ± 2.7 nmol L −1 d −1 , p > 0.05). We found only weak correlations between oxidation rates of urea‐derived N and the abundance or transcription of putative Thaumarchaeota ureC genes. Dependence on urea‐derived N does not appear to be directly related to pH or ammonium concentrations. Competition experiments and release of 15 NH 3 suggest that urea is hydrolyzed to ammonia intracellularly, then a portion is lost to the dissolved pool. The contribution of urea‐derived N to nitrification appears to be minor in temperate coastal waters, but may represent a significant portion of the nitrification flux in Antarctic coastal waters. |
author2 |
Division of Ocean Sciences |
format |
Article in Journal/Newspaper |
author |
Tolar, Bradley B. Wallsgrove, Natalie J. Popp, Brian N. Hollibaugh, James T. |
spellingShingle |
Tolar, Bradley B. Wallsgrove, Natalie J. Popp, Brian N. Hollibaugh, James T. Oxidation of urea‐derived nitrogen by thaumarchaeota‐dominated marine nitrifying communities |
author_facet |
Tolar, Bradley B. Wallsgrove, Natalie J. Popp, Brian N. Hollibaugh, James T. |
author_sort |
Tolar, Bradley B. |
title |
Oxidation of urea‐derived nitrogen by thaumarchaeota‐dominated marine nitrifying communities |
title_short |
Oxidation of urea‐derived nitrogen by thaumarchaeota‐dominated marine nitrifying communities |
title_full |
Oxidation of urea‐derived nitrogen by thaumarchaeota‐dominated marine nitrifying communities |
title_fullStr |
Oxidation of urea‐derived nitrogen by thaumarchaeota‐dominated marine nitrifying communities |
title_full_unstemmed |
Oxidation of urea‐derived nitrogen by thaumarchaeota‐dominated marine nitrifying communities |
title_sort |
oxidation of urea‐derived nitrogen by thaumarchaeota‐dominated marine nitrifying communities |
publisher |
Wiley |
publishDate |
2016 |
url |
http://dx.doi.org/10.1111/1462-2920.13457 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1462-2920.13457 http://api.wiley.com/onlinelibrary/chorus/v1/articles/10.1111%2F1462-2920.13457 https://sfamjournals.onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.13457 http://onlinelibrary.wiley.com/wol1/doi/10.1111/1462-2920.13457/fullpdf |
genre |
Antarc* Antarctic Alaska |
genre_facet |
Antarc* Antarctic Alaska |
op_source |
Environmental Microbiology volume 19, issue 12, page 4838-4850 ISSN 1462-2912 1462-2920 |
op_rights |
http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ |
op_doi |
https://doi.org/10.1111/1462-2920.13457 |
container_title |
Environmental Microbiology |
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19 |
container_issue |
12 |
container_start_page |
4838 |
op_container_end_page |
4850 |
_version_ |
1810492513425817600 |