Polysaccharide utilisation loci of Bacteroidetes from two contrasting open ocean sites in the North Atlantic
Summary Marine Bacteroidetes have pronounced capabilities of degrading high molecular weight organic matter such as proteins and polysaccharides. Previously we reported on 76 Bacteroidetes ‐affiliated fosmids from the North Atlantic Ocean's boreal polar and oligotrophic subtropical provinces. H...
Published in: | Environmental Microbiology |
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crwiley:10.1111/1462-2920.13429 2024-09-15T18:04:20+00:00 Polysaccharide utilisation loci of Bacteroidetes from two contrasting open ocean sites in the North Atlantic Bennke, Christin M. Krüger, Karen Kappelmann, Lennart Huang, Sixing Gobet, Angélique Schüler, Margarete Barbe, Valérie Fuchs, Bernhard M. Michel, Gurvan Teeling, Hanno Amann, Rudolf I. National Research Agency of the French Government by the ‘Blue Enzymes’ ANR project Max-Planck-Society German Science Foundation (DFG) FP6 EU program Network of Excellence Marine Genomics Europe 2016 http://dx.doi.org/10.1111/1462-2920.13429 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1462-2920.13429 http://onlinelibrary.wiley.com/wol1/doi/10.1111/1462-2920.13429/fullpdf en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Microbiology volume 18, issue 12, page 4456-4470 ISSN 1462-2912 1462-2920 journal-article 2016 crwiley https://doi.org/10.1111/1462-2920.13429 2024-08-15T04:19:46Z Summary Marine Bacteroidetes have pronounced capabilities of degrading high molecular weight organic matter such as proteins and polysaccharides. Previously we reported on 76 Bacteroidetes ‐affiliated fosmids from the North Atlantic Ocean's boreal polar and oligotrophic subtropical provinces. Here, we report on the analysis of further 174 fosmids from the same libraries. The combined, re‐assembled dataset (226 contigs; 8.8 Mbp) suggests that planktonic Bacteroidetes at the oligotrophic southern station use more peptides and bacterial and animal polysaccharides, whereas Bacteroidetes at the polar station (East‐Greenland Current) use more algal and plant polysaccharides. The latter agrees with higher abundances of algae and terrigenous organic matter, including plant material, at the polar station. Results were corroborated by in‐depth bioinformatic analysis of 14 polysaccharide utilisation loci from both stations, suggesting laminarin‐specificity for four and specificity for sulfated xylans for two loci. In addition, one locus from the polar station supported use of non‐sulfated xylans and mannans, possibly of plant origin. While peptides likely represent a prime source of carbon for Bacteroidetes in open oceans, our data suggest that as yet unstudied clades of these Bacteroidetes have a surprisingly broad capacity for polysaccharide degradation. In particular, laminarin‐specific PULs seem widespread and thus must be regarded as globally important. Article in Journal/Newspaper East Greenland east greenland current Greenland North Atlantic Wiley Online Library Environmental Microbiology 18 12 4456 4470 |
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Open Polar |
collection |
Wiley Online Library |
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crwiley |
language |
English |
description |
Summary Marine Bacteroidetes have pronounced capabilities of degrading high molecular weight organic matter such as proteins and polysaccharides. Previously we reported on 76 Bacteroidetes ‐affiliated fosmids from the North Atlantic Ocean's boreal polar and oligotrophic subtropical provinces. Here, we report on the analysis of further 174 fosmids from the same libraries. The combined, re‐assembled dataset (226 contigs; 8.8 Mbp) suggests that planktonic Bacteroidetes at the oligotrophic southern station use more peptides and bacterial and animal polysaccharides, whereas Bacteroidetes at the polar station (East‐Greenland Current) use more algal and plant polysaccharides. The latter agrees with higher abundances of algae and terrigenous organic matter, including plant material, at the polar station. Results were corroborated by in‐depth bioinformatic analysis of 14 polysaccharide utilisation loci from both stations, suggesting laminarin‐specificity for four and specificity for sulfated xylans for two loci. In addition, one locus from the polar station supported use of non‐sulfated xylans and mannans, possibly of plant origin. While peptides likely represent a prime source of carbon for Bacteroidetes in open oceans, our data suggest that as yet unstudied clades of these Bacteroidetes have a surprisingly broad capacity for polysaccharide degradation. In particular, laminarin‐specific PULs seem widespread and thus must be regarded as globally important. |
author2 |
National Research Agency of the French Government by the ‘Blue Enzymes’ ANR project Max-Planck-Society German Science Foundation (DFG) FP6 EU program Network of Excellence Marine Genomics Europe |
format |
Article in Journal/Newspaper |
author |
Bennke, Christin M. Krüger, Karen Kappelmann, Lennart Huang, Sixing Gobet, Angélique Schüler, Margarete Barbe, Valérie Fuchs, Bernhard M. Michel, Gurvan Teeling, Hanno Amann, Rudolf I. |
spellingShingle |
Bennke, Christin M. Krüger, Karen Kappelmann, Lennart Huang, Sixing Gobet, Angélique Schüler, Margarete Barbe, Valérie Fuchs, Bernhard M. Michel, Gurvan Teeling, Hanno Amann, Rudolf I. Polysaccharide utilisation loci of Bacteroidetes from two contrasting open ocean sites in the North Atlantic |
author_facet |
Bennke, Christin M. Krüger, Karen Kappelmann, Lennart Huang, Sixing Gobet, Angélique Schüler, Margarete Barbe, Valérie Fuchs, Bernhard M. Michel, Gurvan Teeling, Hanno Amann, Rudolf I. |
author_sort |
Bennke, Christin M. |
title |
Polysaccharide utilisation loci of Bacteroidetes from two contrasting open ocean sites in the North Atlantic |
title_short |
Polysaccharide utilisation loci of Bacteroidetes from two contrasting open ocean sites in the North Atlantic |
title_full |
Polysaccharide utilisation loci of Bacteroidetes from two contrasting open ocean sites in the North Atlantic |
title_fullStr |
Polysaccharide utilisation loci of Bacteroidetes from two contrasting open ocean sites in the North Atlantic |
title_full_unstemmed |
Polysaccharide utilisation loci of Bacteroidetes from two contrasting open ocean sites in the North Atlantic |
title_sort |
polysaccharide utilisation loci of bacteroidetes from two contrasting open ocean sites in the north atlantic |
publisher |
Wiley |
publishDate |
2016 |
url |
http://dx.doi.org/10.1111/1462-2920.13429 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1462-2920.13429 http://onlinelibrary.wiley.com/wol1/doi/10.1111/1462-2920.13429/fullpdf |
genre |
East Greenland east greenland current Greenland North Atlantic |
genre_facet |
East Greenland east greenland current Greenland North Atlantic |
op_source |
Environmental Microbiology volume 18, issue 12, page 4456-4470 ISSN 1462-2912 1462-2920 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/1462-2920.13429 |
container_title |
Environmental Microbiology |
container_volume |
18 |
container_issue |
12 |
container_start_page |
4456 |
op_container_end_page |
4470 |
_version_ |
1810441823647170560 |