Culture‐independent characterization of novel psychrophilic magnetotactic cocci from Antarctic marine sediments
Summary Magnetotactic bacteria (MTB) are a heterogeneous group of ubiquitous aquatic microorganisms capable of biomineralizing nano‐sized, membrane‐bound, magnetic iron‐rich mineral particles called magnetosomes. MTB are found in chemically‐stratified aquatic sediments and/or water columns with a wi...
Published in: | Environmental Microbiology |
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Online Access: | http://dx.doi.org/10.1111/1462-2920.13388 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1462-2920.13388 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.13388 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.13388 https://sfamjournals.onlinelibrary.wiley.com/doi/am-pdf/10.1111/1462-2920.13388 |
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crwiley:10.1111/1462-2920.13388 2024-06-02T07:57:31+00:00 Culture‐independent characterization of novel psychrophilic magnetotactic cocci from Antarctic marine sediments Abreu, Fernanda Carolina, Ana Araujo, V. Leão, Pedro Silva, Karen Tavares Carvalho, Fabíola Marques de Cunha, Oberdan de Lima Almeida, Luiz Gonzaga Geurink, Corey Farina, Marcos Rodelli, Daniel Jovane, Luigi Pellizari, Vivian H. Vasconcelos, Ana Tereza de Bazylinski, Dennis A. Lins, Ulysses Conselho Nacional de Desenvolvimento Científico e Tecnológico Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro Fundação de Amparo à Pesquisa do Estado de São Paulo National Science Foundation 2016 http://dx.doi.org/10.1111/1462-2920.13388 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1462-2920.13388 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.13388 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.13388 https://sfamjournals.onlinelibrary.wiley.com/doi/am-pdf/10.1111/1462-2920.13388 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#am http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Microbiology volume 18, issue 12, page 4426-4441 ISSN 1462-2912 1462-2920 journal-article 2016 crwiley https://doi.org/10.1111/1462-2920.13388 2024-05-03T11:26:18Z Summary Magnetotactic bacteria (MTB) are a heterogeneous group of ubiquitous aquatic microorganisms capable of biomineralizing nano‐sized, membrane‐bound, magnetic iron‐rich mineral particles called magnetosomes. MTB are found in chemically‐stratified aquatic sediments and/or water columns with a wide range of salinities, moderate to high temperatures, and pH varying from neutral to strongly alkaline. MTB from very cold environments have not been investigated to any great degree and here we characterize MTB from the low temperature Antarctic maritime region. Sediment samples were collected at nine sampling sites within Admiralty Bay, King George Island (62°23′S 58°27′W) from 2009 to 2013. Samples from five sites contained MTB and those from two of these sites contained large number of magnetotactic cocci that were studied using electron microscopy and molecular techniques. The magnetotactic cocci contained magnetosomes either arranged as two or four chains or as a disorganized cluster. The crystalline habit and composition of all magnetosomes analyzed with high‐resolution transmission electron microscopy and energy dispersive X‐ray microanalysis were consistent with elongated prismatic crystals of magnetite (Fe 3 O 4 ). The retrieved 16S rRNA gene sequences from magnetically‐enriched magnetotactic cocci clustered into three distinct groups affiliated with the Alphaproteobacteria class of the Proteobacteria . Novel sequences of each phylogenetic cluster were confirmed using fluorescent in situ hybridization. Metagenomic data analysis of magnetically‐enriched magnetotactic cocci revealed the presence of mam genes and MTB‐specific hypothetical protein coding genes. Sequence homology and phylogenetic analysis indicated that predicted proteins are related to those of cultivated alphaproteobacterial MTB. The consistent and continuous low temperature of the sediment where the magnetotactic cocci are present (always below 1°C) suggests that these MTB from maritime Antarctica are psychrophiles. Moreover, similar ... Article in Journal/Newspaper Antarc* Antarctic Antarctica King George Island Wiley Online Library Admiralty Bay Antarctic King George Island Environmental Microbiology 18 12 4426 4441 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Summary Magnetotactic bacteria (MTB) are a heterogeneous group of ubiquitous aquatic microorganisms capable of biomineralizing nano‐sized, membrane‐bound, magnetic iron‐rich mineral particles called magnetosomes. MTB are found in chemically‐stratified aquatic sediments and/or water columns with a wide range of salinities, moderate to high temperatures, and pH varying from neutral to strongly alkaline. MTB from very cold environments have not been investigated to any great degree and here we characterize MTB from the low temperature Antarctic maritime region. Sediment samples were collected at nine sampling sites within Admiralty Bay, King George Island (62°23′S 58°27′W) from 2009 to 2013. Samples from five sites contained MTB and those from two of these sites contained large number of magnetotactic cocci that were studied using electron microscopy and molecular techniques. The magnetotactic cocci contained magnetosomes either arranged as two or four chains or as a disorganized cluster. The crystalline habit and composition of all magnetosomes analyzed with high‐resolution transmission electron microscopy and energy dispersive X‐ray microanalysis were consistent with elongated prismatic crystals of magnetite (Fe 3 O 4 ). The retrieved 16S rRNA gene sequences from magnetically‐enriched magnetotactic cocci clustered into three distinct groups affiliated with the Alphaproteobacteria class of the Proteobacteria . Novel sequences of each phylogenetic cluster were confirmed using fluorescent in situ hybridization. Metagenomic data analysis of magnetically‐enriched magnetotactic cocci revealed the presence of mam genes and MTB‐specific hypothetical protein coding genes. Sequence homology and phylogenetic analysis indicated that predicted proteins are related to those of cultivated alphaproteobacterial MTB. The consistent and continuous low temperature of the sediment where the magnetotactic cocci are present (always below 1°C) suggests that these MTB from maritime Antarctica are psychrophiles. Moreover, similar ... |
author2 |
Conselho Nacional de Desenvolvimento Científico e Tecnológico Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro Fundação de Amparo à Pesquisa do Estado de São Paulo National Science Foundation |
format |
Article in Journal/Newspaper |
author |
Abreu, Fernanda Carolina, Ana Araujo, V. Leão, Pedro Silva, Karen Tavares Carvalho, Fabíola Marques de Cunha, Oberdan de Lima Almeida, Luiz Gonzaga Geurink, Corey Farina, Marcos Rodelli, Daniel Jovane, Luigi Pellizari, Vivian H. Vasconcelos, Ana Tereza de Bazylinski, Dennis A. Lins, Ulysses |
spellingShingle |
Abreu, Fernanda Carolina, Ana Araujo, V. Leão, Pedro Silva, Karen Tavares Carvalho, Fabíola Marques de Cunha, Oberdan de Lima Almeida, Luiz Gonzaga Geurink, Corey Farina, Marcos Rodelli, Daniel Jovane, Luigi Pellizari, Vivian H. Vasconcelos, Ana Tereza de Bazylinski, Dennis A. Lins, Ulysses Culture‐independent characterization of novel psychrophilic magnetotactic cocci from Antarctic marine sediments |
author_facet |
Abreu, Fernanda Carolina, Ana Araujo, V. Leão, Pedro Silva, Karen Tavares Carvalho, Fabíola Marques de Cunha, Oberdan de Lima Almeida, Luiz Gonzaga Geurink, Corey Farina, Marcos Rodelli, Daniel Jovane, Luigi Pellizari, Vivian H. Vasconcelos, Ana Tereza de Bazylinski, Dennis A. Lins, Ulysses |
author_sort |
Abreu, Fernanda |
title |
Culture‐independent characterization of novel psychrophilic magnetotactic cocci from Antarctic marine sediments |
title_short |
Culture‐independent characterization of novel psychrophilic magnetotactic cocci from Antarctic marine sediments |
title_full |
Culture‐independent characterization of novel psychrophilic magnetotactic cocci from Antarctic marine sediments |
title_fullStr |
Culture‐independent characterization of novel psychrophilic magnetotactic cocci from Antarctic marine sediments |
title_full_unstemmed |
Culture‐independent characterization of novel psychrophilic magnetotactic cocci from Antarctic marine sediments |
title_sort |
culture‐independent characterization of novel psychrophilic magnetotactic cocci from antarctic marine sediments |
publisher |
Wiley |
publishDate |
2016 |
url |
http://dx.doi.org/10.1111/1462-2920.13388 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1462-2920.13388 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.13388 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.13388 https://sfamjournals.onlinelibrary.wiley.com/doi/am-pdf/10.1111/1462-2920.13388 |
geographic |
Admiralty Bay Antarctic King George Island |
geographic_facet |
Admiralty Bay Antarctic King George Island |
genre |
Antarc* Antarctic Antarctica King George Island |
genre_facet |
Antarc* Antarctic Antarctica King George Island |
op_source |
Environmental Microbiology volume 18, issue 12, page 4426-4441 ISSN 1462-2912 1462-2920 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#am http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/1462-2920.13388 |
container_title |
Environmental Microbiology |
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18 |
container_issue |
12 |
container_start_page |
4426 |
op_container_end_page |
4441 |
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1800740672031948800 |