RNA viruses as major contributors to Antarctic virioplankton
Summary Early work on marine algal viruses focused exclusively on those having DNA genomes, but recent studies suggest that RNA viruses, especially those with positive‐sense, single‐stranded RNA (+ssRNA) genomes, are abundant in tropical and temperate coastal seawater. To test whether this was also...
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crwiley:10.1111/1462-2920.13291 2024-04-28T08:01:37+00:00 RNA viruses as major contributors to Antarctic virioplankton Miranda, Jaclyn A. Culley, Alexander I. Schvarcz, Christopher R. Steward, Grieg F. 2016 http://dx.doi.org/10.1111/1462-2920.13291 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1462-2920.13291 http://onlinelibrary.wiley.com/wol1/doi/10.1111/1462-2920.13291/fullpdf en eng Wiley http://creativecommons.org/licenses/by-nc-nd/4.0/ Environmental Microbiology volume 18, issue 11, page 3714-3727 ISSN 1462-2912 1462-2920 Ecology, Evolution, Behavior and Systematics Microbiology journal-article 2016 crwiley https://doi.org/10.1111/1462-2920.13291 2024-04-08T06:56:28Z Summary Early work on marine algal viruses focused exclusively on those having DNA genomes, but recent studies suggest that RNA viruses, especially those with positive‐sense, single‐stranded RNA (+ssRNA) genomes, are abundant in tropical and temperate coastal seawater. To test whether this was also true of polar waters, we estimated the relative abundances of RNA and DNA viruses using a mass ratio approach and conducted shotgun metagenomics on purified viral samples collected from a coastal site near Palmer Station, Antarctica on six occasions throughout a summer phytoplankton bloom (November–March). Our data suggest that RNA viruses contributed up to 65% of the total virioplankton (8–65%), and that, as observed previously in warmer waters, the majority of RNA viruses in these Antarctic RNA virus metagenomes had +ssRNA genomes most closely related to viruses in the order Picornavirales . Assembly of the metagenomic reads resulted in five novel, nearly complete genomes, three of which had features similar to diatom‐infecting viruses. Our data are consistent with the hypothesis that RNA viruses influence diatom bloom dynamics in Antarctic waters. Article in Journal/Newspaper Antarc* Antarctic Antarctica Wiley Online Library Environmental Microbiology 18 11 3714 3727 |
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Wiley Online Library |
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crwiley |
language |
English |
topic |
Ecology, Evolution, Behavior and Systematics Microbiology |
spellingShingle |
Ecology, Evolution, Behavior and Systematics Microbiology Miranda, Jaclyn A. Culley, Alexander I. Schvarcz, Christopher R. Steward, Grieg F. RNA viruses as major contributors to Antarctic virioplankton |
topic_facet |
Ecology, Evolution, Behavior and Systematics Microbiology |
description |
Summary Early work on marine algal viruses focused exclusively on those having DNA genomes, but recent studies suggest that RNA viruses, especially those with positive‐sense, single‐stranded RNA (+ssRNA) genomes, are abundant in tropical and temperate coastal seawater. To test whether this was also true of polar waters, we estimated the relative abundances of RNA and DNA viruses using a mass ratio approach and conducted shotgun metagenomics on purified viral samples collected from a coastal site near Palmer Station, Antarctica on six occasions throughout a summer phytoplankton bloom (November–March). Our data suggest that RNA viruses contributed up to 65% of the total virioplankton (8–65%), and that, as observed previously in warmer waters, the majority of RNA viruses in these Antarctic RNA virus metagenomes had +ssRNA genomes most closely related to viruses in the order Picornavirales . Assembly of the metagenomic reads resulted in five novel, nearly complete genomes, three of which had features similar to diatom‐infecting viruses. Our data are consistent with the hypothesis that RNA viruses influence diatom bloom dynamics in Antarctic waters. |
format |
Article in Journal/Newspaper |
author |
Miranda, Jaclyn A. Culley, Alexander I. Schvarcz, Christopher R. Steward, Grieg F. |
author_facet |
Miranda, Jaclyn A. Culley, Alexander I. Schvarcz, Christopher R. Steward, Grieg F. |
author_sort |
Miranda, Jaclyn A. |
title |
RNA viruses as major contributors to Antarctic virioplankton |
title_short |
RNA viruses as major contributors to Antarctic virioplankton |
title_full |
RNA viruses as major contributors to Antarctic virioplankton |
title_fullStr |
RNA viruses as major contributors to Antarctic virioplankton |
title_full_unstemmed |
RNA viruses as major contributors to Antarctic virioplankton |
title_sort |
rna viruses as major contributors to antarctic virioplankton |
publisher |
Wiley |
publishDate |
2016 |
url |
http://dx.doi.org/10.1111/1462-2920.13291 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1462-2920.13291 http://onlinelibrary.wiley.com/wol1/doi/10.1111/1462-2920.13291/fullpdf |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Environmental Microbiology volume 18, issue 11, page 3714-3727 ISSN 1462-2912 1462-2920 |
op_rights |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
op_doi |
https://doi.org/10.1111/1462-2920.13291 |
container_title |
Environmental Microbiology |
container_volume |
18 |
container_issue |
11 |
container_start_page |
3714 |
op_container_end_page |
3727 |
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1797573281966981120 |