Single TRAMdomain RNA‐binding proteins in Archaea: functional insight from Ctr3 from the Antarctic methanogen Methanococcoides burtonii
Summary TRAM domain proteins present in A rchaea and Bacteria have a β‐barrel shape with anti‐parallel β‐sheets that form a nucleic acid binding surface; a structure also present in cold shock proteins ( C sps). Aside from protein structures, experimental data defining the function of TRAM domains i...
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Online Access: | http://dx.doi.org/10.1111/1462-2920.13229 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1462-2920.13229 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.13229 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.13229 https://sfamjournals.onlinelibrary.wiley.com/doi/am-pdf/10.1111/1462-2920.13229 |
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crwiley:10.1111/1462-2920.13229 2024-09-09T19:09:59+00:00 Single TRAMdomain RNA‐binding proteins in Archaea: functional insight from Ctr3 from the Antarctic methanogen Methanococcoides burtonii Taha Siddiqui, K. S. Campanaro, S. Najnin, T. Deshpande, N. Williams, T. J. Aldrich‐Wright, J. Wilkins, M. Curmi, P. M. G. Cavicchioli, R. Australian Research Council 2016 http://dx.doi.org/10.1111/1462-2920.13229 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1462-2920.13229 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.13229 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.13229 https://sfamjournals.onlinelibrary.wiley.com/doi/am-pdf/10.1111/1462-2920.13229 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#am http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Microbiology volume 18, issue 9, page 2810-2824 ISSN 1462-2912 1462-2920 journal-article 2016 crwiley https://doi.org/10.1111/1462-2920.13229 2024-06-18T04:13:01Z Summary TRAM domain proteins present in A rchaea and Bacteria have a β‐barrel shape with anti‐parallel β‐sheets that form a nucleic acid binding surface; a structure also present in cold shock proteins ( C sps). Aside from protein structures, experimental data defining the function of TRAM domains is lacking. Here, we explore the possible functional properties of a single TRAM domain protein, C tr3 ( c old‐responsive TR AM domain protein 3 ) from the Antarctic archaeon M ethanococcoides burtonii that has increased abundance during low temperature growth. Ribonucleic acid ( RNA ) bound by C tr3 in vitro was determined using RNA ‐seq. C tr3‐bound M . burtonii RNA with a preference for transfer (t) RNA and 5S ribosomal RNA , and a potential binding motif was identified. In t RNA , the motif represented the C loop; a region that is conserved in t RNA from all domains of life and appears to be solvent exposed, potentially providing access for C tr3 to bind. C tr3 and C sps are structurally similar and are both inferred to function in low temperature translation. The broad representation of single TRAM domain proteins within A rchaea compared with their apparent absence in B acteria , and scarcity of C sps in A rchaea but prevalence in B acteria , suggests they represent distinct evolutionary lineages of functionally equivalent RNA ‐binding proteins. Article in Journal/Newspaper Antarc* Antarctic Wiley Online Library Antarctic The Antarctic Environmental Microbiology 18 9 2810 2824 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Summary TRAM domain proteins present in A rchaea and Bacteria have a β‐barrel shape with anti‐parallel β‐sheets that form a nucleic acid binding surface; a structure also present in cold shock proteins ( C sps). Aside from protein structures, experimental data defining the function of TRAM domains is lacking. Here, we explore the possible functional properties of a single TRAM domain protein, C tr3 ( c old‐responsive TR AM domain protein 3 ) from the Antarctic archaeon M ethanococcoides burtonii that has increased abundance during low temperature growth. Ribonucleic acid ( RNA ) bound by C tr3 in vitro was determined using RNA ‐seq. C tr3‐bound M . burtonii RNA with a preference for transfer (t) RNA and 5S ribosomal RNA , and a potential binding motif was identified. In t RNA , the motif represented the C loop; a region that is conserved in t RNA from all domains of life and appears to be solvent exposed, potentially providing access for C tr3 to bind. C tr3 and C sps are structurally similar and are both inferred to function in low temperature translation. The broad representation of single TRAM domain proteins within A rchaea compared with their apparent absence in B acteria , and scarcity of C sps in A rchaea but prevalence in B acteria , suggests they represent distinct evolutionary lineages of functionally equivalent RNA ‐binding proteins. |
author2 |
Australian Research Council |
format |
Article in Journal/Newspaper |
author |
Taha Siddiqui, K. S. Campanaro, S. Najnin, T. Deshpande, N. Williams, T. J. Aldrich‐Wright, J. Wilkins, M. Curmi, P. M. G. Cavicchioli, R. |
spellingShingle |
Taha Siddiqui, K. S. Campanaro, S. Najnin, T. Deshpande, N. Williams, T. J. Aldrich‐Wright, J. Wilkins, M. Curmi, P. M. G. Cavicchioli, R. Single TRAMdomain RNA‐binding proteins in Archaea: functional insight from Ctr3 from the Antarctic methanogen Methanococcoides burtonii |
author_facet |
Taha Siddiqui, K. S. Campanaro, S. Najnin, T. Deshpande, N. Williams, T. J. Aldrich‐Wright, J. Wilkins, M. Curmi, P. M. G. Cavicchioli, R. |
author_sort |
Taha |
title |
Single TRAMdomain RNA‐binding proteins in Archaea: functional insight from Ctr3 from the Antarctic methanogen Methanococcoides burtonii |
title_short |
Single TRAMdomain RNA‐binding proteins in Archaea: functional insight from Ctr3 from the Antarctic methanogen Methanococcoides burtonii |
title_full |
Single TRAMdomain RNA‐binding proteins in Archaea: functional insight from Ctr3 from the Antarctic methanogen Methanococcoides burtonii |
title_fullStr |
Single TRAMdomain RNA‐binding proteins in Archaea: functional insight from Ctr3 from the Antarctic methanogen Methanococcoides burtonii |
title_full_unstemmed |
Single TRAMdomain RNA‐binding proteins in Archaea: functional insight from Ctr3 from the Antarctic methanogen Methanococcoides burtonii |
title_sort |
single tramdomain rna‐binding proteins in archaea: functional insight from ctr3 from the antarctic methanogen methanococcoides burtonii |
publisher |
Wiley |
publishDate |
2016 |
url |
http://dx.doi.org/10.1111/1462-2920.13229 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1462-2920.13229 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.13229 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.13229 https://sfamjournals.onlinelibrary.wiley.com/doi/am-pdf/10.1111/1462-2920.13229 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Environmental Microbiology volume 18, issue 9, page 2810-2824 ISSN 1462-2912 1462-2920 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#am http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/1462-2920.13229 |
container_title |
Environmental Microbiology |
container_volume |
18 |
container_issue |
9 |
container_start_page |
2810 |
op_container_end_page |
2824 |
_version_ |
1809824304743841792 |