The role of tissue‐specific microbiota in initial establishment success of Pacific oysters
Summary Microbiota can have positive and negative effects on hosts depending on the environmental conditions. Therefore, it is important to decipher host–microbiota–environment interactions, especially under natural conditions exerting (a)biotic stress. Here, we assess the relative importance of mic...
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crwiley:10.1111/1462-2920.13163 2024-10-13T14:10:08+00:00 The role of tissue‐specific microbiota in initial establishment success of Pacific oysters Lokmer, Ana Kuenzel, Sven Baines, John F. Wegner, Karl Mathias Deutsche Forschungsgemeinschaft International Max Planck Research School for Evolutionary Biology 2016 http://dx.doi.org/10.1111/1462-2920.13163 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1462-2920.13163 http://onlinelibrary.wiley.com/wol1/doi/10.1111/1462-2920.13163/fullpdf en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Microbiology volume 18, issue 3, page 970-987 ISSN 1462-2912 1462-2920 journal-article 2016 crwiley https://doi.org/10.1111/1462-2920.13163 2024-09-17T04:52:06Z Summary Microbiota can have positive and negative effects on hosts depending on the environmental conditions. Therefore, it is important to decipher host–microbiota–environment interactions, especially under natural conditions exerting (a)biotic stress. Here, we assess the relative importance of microbiota in different tissues of Pacific oyster for its successful establishment in a new environment. We transplanted oysters from the Southern to the N orthern W adden S ea and controlled for the effects of resident microbiota by administering antibiotics to half of the oysters. We then followed survival and composition of haemolymph, mantle, gill and gut microbiota in local and translocated oysters over 5 days. High mortality was recorded only in non‐antibiotic‐treated translocated oysters, where high titres of active V ibrio sp. in solid tissues indicated systemic infections. Network analyses revealed the highest connectivity and a link to seawater communities in the haemolymph microbiota. Since antibiotics decreased modularity and increased connectivity of the haemolymph‐based networks, we propose that community destabilization in non‐treated translocated oysters could be attributed to interactions between resident and external microbiota, which in turn facilitated passage of vibrios into solid tissues and invoked disease. These interactions of haemolymph microbiota with the external and internal environment may thus represent an important component of oyster fitness. Article in Journal/Newspaper Pacific oyster Wiley Online Library Pacific Environmental Microbiology 18 3 970 987 |
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Wiley Online Library |
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crwiley |
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English |
description |
Summary Microbiota can have positive and negative effects on hosts depending on the environmental conditions. Therefore, it is important to decipher host–microbiota–environment interactions, especially under natural conditions exerting (a)biotic stress. Here, we assess the relative importance of microbiota in different tissues of Pacific oyster for its successful establishment in a new environment. We transplanted oysters from the Southern to the N orthern W adden S ea and controlled for the effects of resident microbiota by administering antibiotics to half of the oysters. We then followed survival and composition of haemolymph, mantle, gill and gut microbiota in local and translocated oysters over 5 days. High mortality was recorded only in non‐antibiotic‐treated translocated oysters, where high titres of active V ibrio sp. in solid tissues indicated systemic infections. Network analyses revealed the highest connectivity and a link to seawater communities in the haemolymph microbiota. Since antibiotics decreased modularity and increased connectivity of the haemolymph‐based networks, we propose that community destabilization in non‐treated translocated oysters could be attributed to interactions between resident and external microbiota, which in turn facilitated passage of vibrios into solid tissues and invoked disease. These interactions of haemolymph microbiota with the external and internal environment may thus represent an important component of oyster fitness. |
author2 |
Deutsche Forschungsgemeinschaft International Max Planck Research School for Evolutionary Biology |
format |
Article in Journal/Newspaper |
author |
Lokmer, Ana Kuenzel, Sven Baines, John F. Wegner, Karl Mathias |
spellingShingle |
Lokmer, Ana Kuenzel, Sven Baines, John F. Wegner, Karl Mathias The role of tissue‐specific microbiota in initial establishment success of Pacific oysters |
author_facet |
Lokmer, Ana Kuenzel, Sven Baines, John F. Wegner, Karl Mathias |
author_sort |
Lokmer, Ana |
title |
The role of tissue‐specific microbiota in initial establishment success of Pacific oysters |
title_short |
The role of tissue‐specific microbiota in initial establishment success of Pacific oysters |
title_full |
The role of tissue‐specific microbiota in initial establishment success of Pacific oysters |
title_fullStr |
The role of tissue‐specific microbiota in initial establishment success of Pacific oysters |
title_full_unstemmed |
The role of tissue‐specific microbiota in initial establishment success of Pacific oysters |
title_sort |
role of tissue‐specific microbiota in initial establishment success of pacific oysters |
publisher |
Wiley |
publishDate |
2016 |
url |
http://dx.doi.org/10.1111/1462-2920.13163 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1462-2920.13163 http://onlinelibrary.wiley.com/wol1/doi/10.1111/1462-2920.13163/fullpdf |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Pacific oyster |
genre_facet |
Pacific oyster |
op_source |
Environmental Microbiology volume 18, issue 3, page 970-987 ISSN 1462-2912 1462-2920 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/1462-2920.13163 |
container_title |
Environmental Microbiology |
container_volume |
18 |
container_issue |
3 |
container_start_page |
970 |
op_container_end_page |
987 |
_version_ |
1812817286169362432 |