Methane‐derived carbon flow through microbial communities in arctic lake sediments
Summary Aerobic methane ( CH 4 ) oxidation mitigates CH 4 release and is a significant pathway for carbon and energy flow into aquatic food webs. Arctic lakes are responsible for an increasing proportion of global CH 4 emissions, but CH 4 assimilation into the aquatic food web in arctic lakes is poo...
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Online Access: | http://dx.doi.org/10.1111/1462-2920.12773 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1462-2920.12773 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.12773 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.12773 https://sfamjournals.onlinelibrary.wiley.com/doi/am-pdf/10.1111/1462-2920.12773 |
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crwiley:10.1111/1462-2920.12773 2024-04-14T08:06:03+00:00 Methane‐derived carbon flow through microbial communities in arctic lake sediments He, Ruo Wooller, Matthew J. Pohlman, John W. Tiedje, James M. Leigh, Mary Beth Office of Fossil Energy 2015 http://dx.doi.org/10.1111/1462-2920.12773 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1462-2920.12773 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.12773 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.12773 https://sfamjournals.onlinelibrary.wiley.com/doi/am-pdf/10.1111/1462-2920.12773 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#am http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Microbiology volume 17, issue 9, page 3233-3250 ISSN 1462-2912 1462-2920 Ecology, Evolution, Behavior and Systematics Microbiology journal-article 2015 crwiley https://doi.org/10.1111/1462-2920.12773 2024-03-26T09:19:33Z Summary Aerobic methane ( CH 4 ) oxidation mitigates CH 4 release and is a significant pathway for carbon and energy flow into aquatic food webs. Arctic lakes are responsible for an increasing proportion of global CH 4 emissions, but CH 4 assimilation into the aquatic food web in arctic lakes is poorly understood. Using stable isotope probing ( SIP ) based on phospholipid fatty acids ( PLFA‐SIP ) and DNA ( DNA‐SIP ), we tracked carbon flow quantitatively from CH 4 into sediment microorganisms from an arctic lake with an active CH 4 seepage. When 0.025 mmol CH 4 g −1 wet sediment was oxidized, approximately 15.8–32.8% of the CH 4 ‐derived carbon had been incorporated into microorganisms. This CH 4 ‐derived carbon equated to up to 5.7% of total primary production estimates for Alaskan arctic lakes. Type I methanotrophs, including Methylomonas , Methylobacter and unclassified Methylococcaceae , were most active at CH 4 oxidation in this arctic lake. With increasing distance from the active CH 4 seepage, a greater diversity of bacteria incorporated CH 4 ‐derived carbon. Actinomycetes were the most quantitatively important microorganisms involved in secondary feeding on CH 4 ‐derived carbon. These results showed that CH 4 flows through methanotrophs into the broader microbial community and that type I methanotrophs, methylotrophs and actinomycetes are important organisms involved in using CH 4 ‐derived carbon in arctic freshwater ecosystems. Article in Journal/Newspaper Arctic Wiley Online Library Arctic Arctic Lake ENVELOPE(-130.826,-130.826,57.231,57.231) Environmental Microbiology 17 9 3233 3250 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
topic |
Ecology, Evolution, Behavior and Systematics Microbiology |
spellingShingle |
Ecology, Evolution, Behavior and Systematics Microbiology He, Ruo Wooller, Matthew J. Pohlman, John W. Tiedje, James M. Leigh, Mary Beth Methane‐derived carbon flow through microbial communities in arctic lake sediments |
topic_facet |
Ecology, Evolution, Behavior and Systematics Microbiology |
description |
Summary Aerobic methane ( CH 4 ) oxidation mitigates CH 4 release and is a significant pathway for carbon and energy flow into aquatic food webs. Arctic lakes are responsible for an increasing proportion of global CH 4 emissions, but CH 4 assimilation into the aquatic food web in arctic lakes is poorly understood. Using stable isotope probing ( SIP ) based on phospholipid fatty acids ( PLFA‐SIP ) and DNA ( DNA‐SIP ), we tracked carbon flow quantitatively from CH 4 into sediment microorganisms from an arctic lake with an active CH 4 seepage. When 0.025 mmol CH 4 g −1 wet sediment was oxidized, approximately 15.8–32.8% of the CH 4 ‐derived carbon had been incorporated into microorganisms. This CH 4 ‐derived carbon equated to up to 5.7% of total primary production estimates for Alaskan arctic lakes. Type I methanotrophs, including Methylomonas , Methylobacter and unclassified Methylococcaceae , were most active at CH 4 oxidation in this arctic lake. With increasing distance from the active CH 4 seepage, a greater diversity of bacteria incorporated CH 4 ‐derived carbon. Actinomycetes were the most quantitatively important microorganisms involved in secondary feeding on CH 4 ‐derived carbon. These results showed that CH 4 flows through methanotrophs into the broader microbial community and that type I methanotrophs, methylotrophs and actinomycetes are important organisms involved in using CH 4 ‐derived carbon in arctic freshwater ecosystems. |
author2 |
Office of Fossil Energy |
format |
Article in Journal/Newspaper |
author |
He, Ruo Wooller, Matthew J. Pohlman, John W. Tiedje, James M. Leigh, Mary Beth |
author_facet |
He, Ruo Wooller, Matthew J. Pohlman, John W. Tiedje, James M. Leigh, Mary Beth |
author_sort |
He, Ruo |
title |
Methane‐derived carbon flow through microbial communities in arctic lake sediments |
title_short |
Methane‐derived carbon flow through microbial communities in arctic lake sediments |
title_full |
Methane‐derived carbon flow through microbial communities in arctic lake sediments |
title_fullStr |
Methane‐derived carbon flow through microbial communities in arctic lake sediments |
title_full_unstemmed |
Methane‐derived carbon flow through microbial communities in arctic lake sediments |
title_sort |
methane‐derived carbon flow through microbial communities in arctic lake sediments |
publisher |
Wiley |
publishDate |
2015 |
url |
http://dx.doi.org/10.1111/1462-2920.12773 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1462-2920.12773 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.12773 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.12773 https://sfamjournals.onlinelibrary.wiley.com/doi/am-pdf/10.1111/1462-2920.12773 |
long_lat |
ENVELOPE(-130.826,-130.826,57.231,57.231) |
geographic |
Arctic Arctic Lake |
geographic_facet |
Arctic Arctic Lake |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Environmental Microbiology volume 17, issue 9, page 3233-3250 ISSN 1462-2912 1462-2920 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#am http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/1462-2920.12773 |
container_title |
Environmental Microbiology |
container_volume |
17 |
container_issue |
9 |
container_start_page |
3233 |
op_container_end_page |
3250 |
_version_ |
1796302672537583616 |