Isolation and physiological characterization of psychrophilic denitrifying bacteria from permanently cold Arctic fjord sediments ( Svalbard, Norway)
Summary A large proportion of reactive nitrogen loss from polar sediments is mediated by denitrification, but microorganisms mediating denitrification in polar environments remain poorly characterized. A combined approach of most‐probable‐number ( MPN ) enumeration, cultivation and physiological cha...
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crwiley:10.1111/1462-2920.12110 2024-06-23T07:50:35+00:00 Isolation and physiological characterization of psychrophilic denitrifying bacteria from permanently cold Arctic fjord sediments ( Svalbard, Norway) Canion, Andy Prakash, Om Green, Stefan J. Jahnke, Linda Kuypers, Marcel M. M. Kostka, Joel E. 2013 http://dx.doi.org/10.1111/1462-2920.12110 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1462-2920.12110 http://onlinelibrary.wiley.com/wol1/doi/10.1111/1462-2920.12110/fullpdf en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Microbiology volume 15, issue 5, page 1606-1618 ISSN 1462-2912 1462-2920 journal-article 2013 crwiley https://doi.org/10.1111/1462-2920.12110 2024-06-04T06:40:45Z Summary A large proportion of reactive nitrogen loss from polar sediments is mediated by denitrification, but microorganisms mediating denitrification in polar environments remain poorly characterized. A combined approach of most‐probable‐number ( MPN ) enumeration, cultivation and physiological characterization was used to describe psychrophilic denitrifying bacterial communities in sediments of three A rctic fjords in S valbard ( N orway). A MPN assay showed the presence of 10 3 −10 6 cells of psychrophilic nitrate‐respiring bacteria g −1 of sediment. Fifteen strains within the P roteobacteria were isolated using a systematic enrichment approach with organic acids as electron donors and nitrate as an electron acceptor. Isolates belonged to five genera, including S hewanella , P seudomonas , P sychromonas ( G ammaproteobacteria ), A rcobacter ( E psilonproteobacteria ) and H erminiimonas ( B etaproteobacteria ) . All isolates were denitrifiers, except S hewanella , which exhibited the capacity for dissimilatory nitrate reduction to ammonium ( DNRA ). Growth from 0 to 40° C demonstrated that all genera except S hewanella were psychrophiles with optimal growth below 15° C , and adaptation to low temperature was demonstrated as a shift from primarily C 16:0 saturated fatty acids to C 16:1 monounsaturated fatty acids at lower temperatures. This study provides the first targeted enrichment and characterization of psychrophilic denitrifying bacteria from polar sediments, and two genera, A rcobacter and H erminiimonas , are isolated for the first time from permanently cold marine sediments. Article in Journal/Newspaper Arctic Svalbard Wiley Online Library Arctic Norway Svalbard Environmental Microbiology 15 5 1606 1618 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Summary A large proportion of reactive nitrogen loss from polar sediments is mediated by denitrification, but microorganisms mediating denitrification in polar environments remain poorly characterized. A combined approach of most‐probable‐number ( MPN ) enumeration, cultivation and physiological characterization was used to describe psychrophilic denitrifying bacterial communities in sediments of three A rctic fjords in S valbard ( N orway). A MPN assay showed the presence of 10 3 −10 6 cells of psychrophilic nitrate‐respiring bacteria g −1 of sediment. Fifteen strains within the P roteobacteria were isolated using a systematic enrichment approach with organic acids as electron donors and nitrate as an electron acceptor. Isolates belonged to five genera, including S hewanella , P seudomonas , P sychromonas ( G ammaproteobacteria ), A rcobacter ( E psilonproteobacteria ) and H erminiimonas ( B etaproteobacteria ) . All isolates were denitrifiers, except S hewanella , which exhibited the capacity for dissimilatory nitrate reduction to ammonium ( DNRA ). Growth from 0 to 40° C demonstrated that all genera except S hewanella were psychrophiles with optimal growth below 15° C , and adaptation to low temperature was demonstrated as a shift from primarily C 16:0 saturated fatty acids to C 16:1 monounsaturated fatty acids at lower temperatures. This study provides the first targeted enrichment and characterization of psychrophilic denitrifying bacteria from polar sediments, and two genera, A rcobacter and H erminiimonas , are isolated for the first time from permanently cold marine sediments. |
format |
Article in Journal/Newspaper |
author |
Canion, Andy Prakash, Om Green, Stefan J. Jahnke, Linda Kuypers, Marcel M. M. Kostka, Joel E. |
spellingShingle |
Canion, Andy Prakash, Om Green, Stefan J. Jahnke, Linda Kuypers, Marcel M. M. Kostka, Joel E. Isolation and physiological characterization of psychrophilic denitrifying bacteria from permanently cold Arctic fjord sediments ( Svalbard, Norway) |
author_facet |
Canion, Andy Prakash, Om Green, Stefan J. Jahnke, Linda Kuypers, Marcel M. M. Kostka, Joel E. |
author_sort |
Canion, Andy |
title |
Isolation and physiological characterization of psychrophilic denitrifying bacteria from permanently cold Arctic fjord sediments ( Svalbard, Norway) |
title_short |
Isolation and physiological characterization of psychrophilic denitrifying bacteria from permanently cold Arctic fjord sediments ( Svalbard, Norway) |
title_full |
Isolation and physiological characterization of psychrophilic denitrifying bacteria from permanently cold Arctic fjord sediments ( Svalbard, Norway) |
title_fullStr |
Isolation and physiological characterization of psychrophilic denitrifying bacteria from permanently cold Arctic fjord sediments ( Svalbard, Norway) |
title_full_unstemmed |
Isolation and physiological characterization of psychrophilic denitrifying bacteria from permanently cold Arctic fjord sediments ( Svalbard, Norway) |
title_sort |
isolation and physiological characterization of psychrophilic denitrifying bacteria from permanently cold arctic fjord sediments ( svalbard, norway) |
publisher |
Wiley |
publishDate |
2013 |
url |
http://dx.doi.org/10.1111/1462-2920.12110 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1462-2920.12110 http://onlinelibrary.wiley.com/wol1/doi/10.1111/1462-2920.12110/fullpdf |
geographic |
Arctic Norway Svalbard |
geographic_facet |
Arctic Norway Svalbard |
genre |
Arctic Svalbard |
genre_facet |
Arctic Svalbard |
op_source |
Environmental Microbiology volume 15, issue 5, page 1606-1618 ISSN 1462-2912 1462-2920 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/1462-2920.12110 |
container_title |
Environmental Microbiology |
container_volume |
15 |
container_issue |
5 |
container_start_page |
1606 |
op_container_end_page |
1618 |
_version_ |
1802641492988133376 |