Quantifying capital versus income breeding: New promise with stable isotope measurements of individual amino acids

• Published in: Journal of Animal Ecology
• Main Authors: Whiteman, John P., Newsome, Seth D., Bustamante, Paco, Cherel, Yves, Hobson, Keith A.
• Other Authors: Institut Universitaire de France, Natural Sciences and Engineering Research Council of Canada, University of New Mexico, Institut Polaire Français Paul Emile Victor
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2020
• Subjects: Aptenodytes forsteri; Emperor penguins
• Online Access: http://dx.doi.org/10.1111/1365-2656.13402; https://onlinelibrary.wiley.com/doi/pdf/10.1111/1365-2656.13402; https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1365-2656.13402; https://besjournals.onlinelibrary.wiley.com/doi/pdf/10.1111/1365-2656.13402

Abstract Capital breeders accumulate nutrients prior to egg development, then use these stores to support offspring development. In contrast, income breeders rely on local nutrients consumed contemporaneously with offspring development. Understanding such nutrient allocations is critical to assessing life‐history strategies and habitat use. Despite the contrast between these strategies, it remains challenging to trace nutrients from endogenous stores or exogenous food intake into offspring. Here, we tested a new solution to this problem. Using tissue samples collected opportunistically from wild emperor penguins Aptenodytes forsteri , which exemplify capital breeding, we hypothesized that the stable carbon (δ 13 C) and nitrogen (δ 15 N) isotope values of individual amino acids (AAs) in endogenous stores (e.g. muscle) and in egg yolk and albumen reflect the nutrient sourcing that distinguishes capital versus income breeding. Unlike other methods, this approach does not require untested assumptions or diet sampling. We found that over half of essential AAs had δ 13 C values that did not differ between muscle and yolk or albumen, suggesting that most of these AAs were directly routed from muscle into eggs. In contrast, almost all non‐essential AAs differed in δ 13 C values between muscle and yolk or between muscle and albumen, suggesting de novo synthesis. Over half of AAs that have labile nitrogen atoms (i.e. ‘trophic’ AA) had higher δ 15 N values in yolk and albumen than in muscle, suggesting that they were transaminated during their routing into egg tissue. This effect was smaller for AAs with less labile nitrogen atoms (i.e. ‘source’ AA). Our results indicate that the δ 15 N offset between trophic‐source AAs (Δ 15 N trophic‐source ) may provide an index of the extent of capital breeding. The value of emperor penguin Δ 15 N Pro‐Phe was higher in yolk and albumen than in muscle, reflecting the mobilization of endogenous stores; in comparison, the value of Δ 15 N Pro‐Phe was similar across muscle and egg tissue in ...