The apoptotic effect of ramalin on human breast cancer cells (657.11)
Ramalin is the compound isolated from Antarctic lichen, Ramalina terebrata . Previous study showed that ramalin has antioxidant and anti‐inflammatory activity. However, the effect of ramalin on MCF‐7 human cancer cells has not been addressed. We investigated the effect of ramalin on cell proliferati...
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Online Access: | http://dx.doi.org/10.1096/fasebj.28.1_supplement.657.11 |
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crwiley:10.1096/fasebj.28.1_supplement.657.11 2024-06-02T07:58:05+00:00 The apoptotic effect of ramalin on human breast cancer cells (657.11) Park, Bongkyun Lee, Chunggi Kim, Jiyun Pyo, Suhkneung 2014 http://dx.doi.org/10.1096/fasebj.28.1_supplement.657.11 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor The FASEB Journal volume 28, issue S1 ISSN 0892-6638 1530-6860 journal-article 2014 crwiley https://doi.org/10.1096/fasebj.28.1_supplement.657.11 2024-05-03T11:38:19Z Ramalin is the compound isolated from Antarctic lichen, Ramalina terebrata . Previous study showed that ramalin has antioxidant and anti‐inflammatory activity. However, the effect of ramalin on MCF‐7 human cancer cells has not been addressed. We investigated the effect of ramalin on cell proliferation, apoptosis, and its mechanism in MCF‐7 cells. MTT assay and western blotting showed that ramalin decreased concentration‐dependently cell proliferation, induced apoptosis related proteins (Bax, Bid, AIF), and activated caspase‐3 and 8, respectively. In addition, Annexin V/PI analysis revealed that apoptotic cells were remarkably increased in ramalin‐treated MCF‐7. Our data also showed that ramalin increased phosphorylation of ERK1/2, p38 and JNK in a concentration‐ and time‐dependent manner. Taken together, ramalin induces apoptosis through Mitogen‐activated protein kinases (MAPKs) and caspase dependent signaling pathways in MCF‐7 cells and may be a potential anticancer agent for the treatment of human breast cancer. Article in Journal/Newspaper Antarc* Antarctic Wiley Online Library Antarctic The FASEB Journal 28 S1 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Ramalin is the compound isolated from Antarctic lichen, Ramalina terebrata . Previous study showed that ramalin has antioxidant and anti‐inflammatory activity. However, the effect of ramalin on MCF‐7 human cancer cells has not been addressed. We investigated the effect of ramalin on cell proliferation, apoptosis, and its mechanism in MCF‐7 cells. MTT assay and western blotting showed that ramalin decreased concentration‐dependently cell proliferation, induced apoptosis related proteins (Bax, Bid, AIF), and activated caspase‐3 and 8, respectively. In addition, Annexin V/PI analysis revealed that apoptotic cells were remarkably increased in ramalin‐treated MCF‐7. Our data also showed that ramalin increased phosphorylation of ERK1/2, p38 and JNK in a concentration‐ and time‐dependent manner. Taken together, ramalin induces apoptosis through Mitogen‐activated protein kinases (MAPKs) and caspase dependent signaling pathways in MCF‐7 cells and may be a potential anticancer agent for the treatment of human breast cancer. |
format |
Article in Journal/Newspaper |
author |
Park, Bongkyun Lee, Chunggi Kim, Jiyun Pyo, Suhkneung |
spellingShingle |
Park, Bongkyun Lee, Chunggi Kim, Jiyun Pyo, Suhkneung The apoptotic effect of ramalin on human breast cancer cells (657.11) |
author_facet |
Park, Bongkyun Lee, Chunggi Kim, Jiyun Pyo, Suhkneung |
author_sort |
Park, Bongkyun |
title |
The apoptotic effect of ramalin on human breast cancer cells (657.11) |
title_short |
The apoptotic effect of ramalin on human breast cancer cells (657.11) |
title_full |
The apoptotic effect of ramalin on human breast cancer cells (657.11) |
title_fullStr |
The apoptotic effect of ramalin on human breast cancer cells (657.11) |
title_full_unstemmed |
The apoptotic effect of ramalin on human breast cancer cells (657.11) |
title_sort |
apoptotic effect of ramalin on human breast cancer cells (657.11) |
publisher |
Wiley |
publishDate |
2014 |
url |
http://dx.doi.org/10.1096/fasebj.28.1_supplement.657.11 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
The FASEB Journal volume 28, issue S1 ISSN 0892-6638 1530-6860 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1096/fasebj.28.1_supplement.657.11 |
container_title |
The FASEB Journal |
container_volume |
28 |
container_issue |
S1 |
_version_ |
1800741337938526208 |