Ramalin suppresses adipocyte differentiation though the MAPKs pathway in 3T3‐L1 preadipocyte (657.10)
Obesity is a main risk factor for several metabolic disorders such as type 2 diabetes, hypertension, and cardiovascular diseases. Obesity is also recognized as a low‐grade chronic inflammation. In the current study, the inhibitory effect of ramalin, isolated from Antarctic lichen, Ramalina terebrata...
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Online Access: | http://dx.doi.org/10.1096/fasebj.28.1_supplement.657.10 |
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crwiley:10.1096/fasebj.28.1_supplement.657.10 2024-06-02T07:57:23+00:00 Ramalin suppresses adipocyte differentiation though the MAPKs pathway in 3T3‐L1 preadipocyte (657.10) Lee, Heeweon Lee, Chunggi Jang, Yeonjung Pyo, Suhkneung 2014 http://dx.doi.org/10.1096/fasebj.28.1_supplement.657.10 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor The FASEB Journal volume 28, issue S1 ISSN 0892-6638 1530-6860 journal-article 2014 crwiley https://doi.org/10.1096/fasebj.28.1_supplement.657.10 2024-05-03T11:55:15Z Obesity is a main risk factor for several metabolic disorders such as type 2 diabetes, hypertension, and cardiovascular diseases. Obesity is also recognized as a low‐grade chronic inflammation. In the current study, the inhibitory effect of ramalin, isolated from Antarctic lichen, Ramalina terebrata , on adipocyte differentiation in 3T3‐L1 cells was examined. Ramalin attenuated the accumulation of lipid droplets stained with Oil red O in non‐toxic concentration. Western blot assay showed that ramalin remarkably inhibited the level of peroxisome proliferator‐activated receptor γ (PPAR γ) and CCAAT/enhancer‐binding protein β (c/EBP β) as well as CCAAT/enhancer‐binding protein α (c/EBP alpha) in a concentration dependent manner. Real‐time PCR assay revealed that the expression of MDI‐induced adipogenesis related genes (GLUT4, aP2, leptin) was significantly suppressed by ramalin. Our data also showed that ramalin significantly decreased phosphorylation of ERK1/2, p38 and JNK in a concentration‐dependent manner. Results from ELISA assay demonstrated that ramalin reduced the production of IL‐6 and TNF‐α. Taken together, ramalin can be a novel therapeutic candidate for controlling adipogenesis in preadipocytes. Article in Journal/Newspaper Antarc* Antarctic Wiley Online Library Antarctic The FASEB Journal 28 S1 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Obesity is a main risk factor for several metabolic disorders such as type 2 diabetes, hypertension, and cardiovascular diseases. Obesity is also recognized as a low‐grade chronic inflammation. In the current study, the inhibitory effect of ramalin, isolated from Antarctic lichen, Ramalina terebrata , on adipocyte differentiation in 3T3‐L1 cells was examined. Ramalin attenuated the accumulation of lipid droplets stained with Oil red O in non‐toxic concentration. Western blot assay showed that ramalin remarkably inhibited the level of peroxisome proliferator‐activated receptor γ (PPAR γ) and CCAAT/enhancer‐binding protein β (c/EBP β) as well as CCAAT/enhancer‐binding protein α (c/EBP alpha) in a concentration dependent manner. Real‐time PCR assay revealed that the expression of MDI‐induced adipogenesis related genes (GLUT4, aP2, leptin) was significantly suppressed by ramalin. Our data also showed that ramalin significantly decreased phosphorylation of ERK1/2, p38 and JNK in a concentration‐dependent manner. Results from ELISA assay demonstrated that ramalin reduced the production of IL‐6 and TNF‐α. Taken together, ramalin can be a novel therapeutic candidate for controlling adipogenesis in preadipocytes. |
format |
Article in Journal/Newspaper |
author |
Lee, Heeweon Lee, Chunggi Jang, Yeonjung Pyo, Suhkneung |
spellingShingle |
Lee, Heeweon Lee, Chunggi Jang, Yeonjung Pyo, Suhkneung Ramalin suppresses adipocyte differentiation though the MAPKs pathway in 3T3‐L1 preadipocyte (657.10) |
author_facet |
Lee, Heeweon Lee, Chunggi Jang, Yeonjung Pyo, Suhkneung |
author_sort |
Lee, Heeweon |
title |
Ramalin suppresses adipocyte differentiation though the MAPKs pathway in 3T3‐L1 preadipocyte (657.10) |
title_short |
Ramalin suppresses adipocyte differentiation though the MAPKs pathway in 3T3‐L1 preadipocyte (657.10) |
title_full |
Ramalin suppresses adipocyte differentiation though the MAPKs pathway in 3T3‐L1 preadipocyte (657.10) |
title_fullStr |
Ramalin suppresses adipocyte differentiation though the MAPKs pathway in 3T3‐L1 preadipocyte (657.10) |
title_full_unstemmed |
Ramalin suppresses adipocyte differentiation though the MAPKs pathway in 3T3‐L1 preadipocyte (657.10) |
title_sort |
ramalin suppresses adipocyte differentiation though the mapks pathway in 3t3‐l1 preadipocyte (657.10) |
publisher |
Wiley |
publishDate |
2014 |
url |
http://dx.doi.org/10.1096/fasebj.28.1_supplement.657.10 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
The FASEB Journal volume 28, issue S1 ISSN 0892-6638 1530-6860 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1096/fasebj.28.1_supplement.657.10 |
container_title |
The FASEB Journal |
container_volume |
28 |
container_issue |
S1 |
_version_ |
1800740539343044608 |