1H‐NMR metabolomic biomarkers of poor outcome after hemorrhagic shock are absent in hibernators (590.1)
Hemorrhagic shock (HS) following trauma is a leading cause of death among persons under the age of 40. During HS, systemic ischemia is followed by reperfusion during medical intervention (I/R) resulting in a disruption of cellular metabolic processes and ultimate tissue and organ dysfunction. Resist...
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crwiley:10.1096/fasebj.28.1_supplement.590.1 2024-06-02T08:02:30+00:00 1H‐NMR metabolomic biomarkers of poor outcome after hemorrhagic shock are absent in hibernators (590.1) Bogren, Lori Murphy, Carl Johnston, Erin Serkova, Natalie Drew, Kelly Medical Research and Materiel Command 2014 http://dx.doi.org/10.1096/fasebj.28.1_supplement.590.1 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor The FASEB Journal volume 28, issue S1 ISSN 0892-6638 1530-6860 journal-article 2014 crwiley https://doi.org/10.1096/fasebj.28.1_supplement.590.1 2024-05-03T11:49:59Z Hemorrhagic shock (HS) following trauma is a leading cause of death among persons under the age of 40. During HS, systemic ischemia is followed by reperfusion during medical intervention (I/R) resulting in a disruption of cellular metabolic processes and ultimate tissue and organ dysfunction. Resistance to I/R injury is a characteristic of hibernating mammals. The present study examined if arctic ground squirrels (AGS) are protected from warm HS‐induced global I/R injury due to the ability to maintain a stable metabolic homeostasis during HS. Rats and AGS were subject to HS by withdrawing blood to maintain a MAP of 35 mmHg for 20 min before reperfusion. Body temperature was maintained at 37±0.5°C. Plasma samples were taken immediately before hemorrhage and three hours after reperfusion and were then analyzed via 1H‐NMR spectroscopy for hydrophilic and lipophilic metabolites. Rats had a qualitative shift in their hydrophilic metabolic fingerprint and had alterations in several metabolites (acetate, alanine, glutamine, histidine, β‐hydroxybutyrate, lactate, lysine, and tyrosine ) during I/R indicative of metabolic shut‐down and organ damage. In contrast, AGS, regardless of season, were able to maintain a qualitative 1H‐NMR metabolic homeostasis with little (winter) or no (summer) changes in quantified metabolites during HS‐induced global I/R. In conclusion, AGS are resistant to disruptions in their metabolic processes and organ damage during warm HS‐ I/R. Grant Funding Source : Supported by USAMRMC W81XWH009‐2‐0134 Article in Journal/Newspaper Arctic Wiley Online Library Arctic The FASEB Journal 28 S1 |
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Open Polar |
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Wiley Online Library |
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crwiley |
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English |
description |
Hemorrhagic shock (HS) following trauma is a leading cause of death among persons under the age of 40. During HS, systemic ischemia is followed by reperfusion during medical intervention (I/R) resulting in a disruption of cellular metabolic processes and ultimate tissue and organ dysfunction. Resistance to I/R injury is a characteristic of hibernating mammals. The present study examined if arctic ground squirrels (AGS) are protected from warm HS‐induced global I/R injury due to the ability to maintain a stable metabolic homeostasis during HS. Rats and AGS were subject to HS by withdrawing blood to maintain a MAP of 35 mmHg for 20 min before reperfusion. Body temperature was maintained at 37±0.5°C. Plasma samples were taken immediately before hemorrhage and three hours after reperfusion and were then analyzed via 1H‐NMR spectroscopy for hydrophilic and lipophilic metabolites. Rats had a qualitative shift in their hydrophilic metabolic fingerprint and had alterations in several metabolites (acetate, alanine, glutamine, histidine, β‐hydroxybutyrate, lactate, lysine, and tyrosine ) during I/R indicative of metabolic shut‐down and organ damage. In contrast, AGS, regardless of season, were able to maintain a qualitative 1H‐NMR metabolic homeostasis with little (winter) or no (summer) changes in quantified metabolites during HS‐induced global I/R. In conclusion, AGS are resistant to disruptions in their metabolic processes and organ damage during warm HS‐ I/R. Grant Funding Source : Supported by USAMRMC W81XWH009‐2‐0134 |
author2 |
Medical Research and Materiel Command |
format |
Article in Journal/Newspaper |
author |
Bogren, Lori Murphy, Carl Johnston, Erin Serkova, Natalie Drew, Kelly |
spellingShingle |
Bogren, Lori Murphy, Carl Johnston, Erin Serkova, Natalie Drew, Kelly 1H‐NMR metabolomic biomarkers of poor outcome after hemorrhagic shock are absent in hibernators (590.1) |
author_facet |
Bogren, Lori Murphy, Carl Johnston, Erin Serkova, Natalie Drew, Kelly |
author_sort |
Bogren, Lori |
title |
1H‐NMR metabolomic biomarkers of poor outcome after hemorrhagic shock are absent in hibernators (590.1) |
title_short |
1H‐NMR metabolomic biomarkers of poor outcome after hemorrhagic shock are absent in hibernators (590.1) |
title_full |
1H‐NMR metabolomic biomarkers of poor outcome after hemorrhagic shock are absent in hibernators (590.1) |
title_fullStr |
1H‐NMR metabolomic biomarkers of poor outcome after hemorrhagic shock are absent in hibernators (590.1) |
title_full_unstemmed |
1H‐NMR metabolomic biomarkers of poor outcome after hemorrhagic shock are absent in hibernators (590.1) |
title_sort |
1h‐nmr metabolomic biomarkers of poor outcome after hemorrhagic shock are absent in hibernators (590.1) |
publisher |
Wiley |
publishDate |
2014 |
url |
http://dx.doi.org/10.1096/fasebj.28.1_supplement.590.1 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
The FASEB Journal volume 28, issue S1 ISSN 0892-6638 1530-6860 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1096/fasebj.28.1_supplement.590.1 |
container_title |
The FASEB Journal |
container_volume |
28 |
container_issue |
S1 |
_version_ |
1800746985486024704 |