Prolonged food deprivation increases mRNA and protein expression of deiodinase 1 & 2, and thyroid hormone receptor β‐1 in a fasting‐adapted mammal
Food deprivation in mammals is typically associated with reduced [thyroid hormone] (TH), and deiodinase content and activity to suppress metabolism, but in hibernating, metabolically quiescent ground squirrels, [TH] increase in the presence of reduced TH receptor content and activity making them cry...
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Online Access: | http://dx.doi.org/10.1096/fasebj.27.1_supplement.937.24 |
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crwiley:10.1096/fasebj.27.1_supplement.937.24 2024-06-02T08:06:01+00:00 Prolonged food deprivation increases mRNA and protein expression of deiodinase 1 & 2, and thyroid hormone receptor β‐1 in a fasting‐adapted mammal Martinez, Bridget Sonanez, Jose Guadalupe Ortiz, Rudy M 2013 http://dx.doi.org/10.1096/fasebj.27.1_supplement.937.24 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor The FASEB Journal volume 27, issue S1 ISSN 0892-6638 1530-6860 journal-article 2013 crwiley https://doi.org/10.1096/fasebj.27.1_supplement.937.24 2024-05-03T10:51:27Z Food deprivation in mammals is typically associated with reduced [thyroid hormone] (TH), and deiodinase content and activity to suppress metabolism, but in hibernating, metabolically quiescent ground squirrels, [TH] increase in the presence of reduced TH receptor content and activity making them cryptically hyperthyroid. However, in prolong‐fasted, metabolically active elephant seal pups, TH levels are maintained, if not elevated. The functional relevance of this apparent paradox is unknown and clearly demonstrates variability in the regulation of [TH] and function in food‐deprived mammals. To address the hypothesis that prolonged fasting (7 weeks) increases the expression of deiodinases (DI1/2) and TH receptor (THrβ‐1), we measured the mRNA expression of these genes in adipose and muscle at 1, 3 and 7 wks fasting. Fasting did not decrease plasma [thyroid stimulating hormone] (TSH), total [tri‐iodothyronine] (tT3), free [T3] (fT3), total [thyroxine] ( tT4) and free [T4] (fT4) suggesting that the hypothalamic‐pituitary‐thyroid (HPT) axis is maintained during fasting. The mRNA and protein (except adipose DI1) expressions of adipose and muscle DI1 & 2 increased suggesting that the mechanisms mediating cellular TH activity are increased with prolonged fasting. Fasting also increased adipose and muscle THrβ‐1 mRNA expression. The data demonstrate a unique, atypical mechanism of TH activity and regulation in mammals adapted to prolonged food deprivation in which the potential responsiveness of peripheral tissues and cellular TH activity are increased, likely to meet the energetic demands of prolonged fasting. This unique cellular response may contribute to the seal's adaptation to prolonged fasting, providing insight to therapies for TH‐related pathologies in humans. Article in Journal/Newspaper Elephant Seal Wiley Online Library The FASEB Journal 27 S1 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Food deprivation in mammals is typically associated with reduced [thyroid hormone] (TH), and deiodinase content and activity to suppress metabolism, but in hibernating, metabolically quiescent ground squirrels, [TH] increase in the presence of reduced TH receptor content and activity making them cryptically hyperthyroid. However, in prolong‐fasted, metabolically active elephant seal pups, TH levels are maintained, if not elevated. The functional relevance of this apparent paradox is unknown and clearly demonstrates variability in the regulation of [TH] and function in food‐deprived mammals. To address the hypothesis that prolonged fasting (7 weeks) increases the expression of deiodinases (DI1/2) and TH receptor (THrβ‐1), we measured the mRNA expression of these genes in adipose and muscle at 1, 3 and 7 wks fasting. Fasting did not decrease plasma [thyroid stimulating hormone] (TSH), total [tri‐iodothyronine] (tT3), free [T3] (fT3), total [thyroxine] ( tT4) and free [T4] (fT4) suggesting that the hypothalamic‐pituitary‐thyroid (HPT) axis is maintained during fasting. The mRNA and protein (except adipose DI1) expressions of adipose and muscle DI1 & 2 increased suggesting that the mechanisms mediating cellular TH activity are increased with prolonged fasting. Fasting also increased adipose and muscle THrβ‐1 mRNA expression. The data demonstrate a unique, atypical mechanism of TH activity and regulation in mammals adapted to prolonged food deprivation in which the potential responsiveness of peripheral tissues and cellular TH activity are increased, likely to meet the energetic demands of prolonged fasting. This unique cellular response may contribute to the seal's adaptation to prolonged fasting, providing insight to therapies for TH‐related pathologies in humans. |
format |
Article in Journal/Newspaper |
author |
Martinez, Bridget Sonanez, Jose Guadalupe Ortiz, Rudy M |
spellingShingle |
Martinez, Bridget Sonanez, Jose Guadalupe Ortiz, Rudy M Prolonged food deprivation increases mRNA and protein expression of deiodinase 1 & 2, and thyroid hormone receptor β‐1 in a fasting‐adapted mammal |
author_facet |
Martinez, Bridget Sonanez, Jose Guadalupe Ortiz, Rudy M |
author_sort |
Martinez, Bridget |
title |
Prolonged food deprivation increases mRNA and protein expression of deiodinase 1 & 2, and thyroid hormone receptor β‐1 in a fasting‐adapted mammal |
title_short |
Prolonged food deprivation increases mRNA and protein expression of deiodinase 1 & 2, and thyroid hormone receptor β‐1 in a fasting‐adapted mammal |
title_full |
Prolonged food deprivation increases mRNA and protein expression of deiodinase 1 & 2, and thyroid hormone receptor β‐1 in a fasting‐adapted mammal |
title_fullStr |
Prolonged food deprivation increases mRNA and protein expression of deiodinase 1 & 2, and thyroid hormone receptor β‐1 in a fasting‐adapted mammal |
title_full_unstemmed |
Prolonged food deprivation increases mRNA and protein expression of deiodinase 1 & 2, and thyroid hormone receptor β‐1 in a fasting‐adapted mammal |
title_sort |
prolonged food deprivation increases mrna and protein expression of deiodinase 1 & 2, and thyroid hormone receptor β‐1 in a fasting‐adapted mammal |
publisher |
Wiley |
publishDate |
2013 |
url |
http://dx.doi.org/10.1096/fasebj.27.1_supplement.937.24 |
genre |
Elephant Seal |
genre_facet |
Elephant Seal |
op_source |
The FASEB Journal volume 27, issue S1 ISSN 0892-6638 1530-6860 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1096/fasebj.27.1_supplement.937.24 |
container_title |
The FASEB Journal |
container_volume |
27 |
container_issue |
S1 |
_version_ |
1800750899134464000 |